The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
권호 표지
DOI QR코드

DOI QR Code

Apoptosis Induction of HCT-15 Cells by Extracts of Undaria pinnatifida with Fermented Micro-organism

미역 발효추출물의 HCT-15 대장암 세포 사멸 유도 효과

  • Kim, Tae-Yun (Department of Herbology, College of Oriental Medicine, Gachon University) ;
  • Han, Hyo-Sang (Department of Health Administration, College of Social Sciences, Joongbu University) ;
  • Lee, Young-Jong (Department of Herbology, College of Oriental Medicine, Gachon University)
  • 김태윤 (가천대학교 한의과대학 본초학교실) ;
  • 한효상 (중부대학교 보건행정학과) ;
  • 이영종 (가천대학교 한의과대학 본초학교실)
  • Received : 2013.06.19
  • Accepted : 2013.07.18
  • Published : 2013.07.30
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives : To study the apoptosis effects of fermented Undaria pinnatifida extracts(FUP) against HCT-15 colon cancer cells. Method : By measuring cell proliferation, DNA fragmentation, cell cycle, morphology, and western blot from FUP, the study investigated the effects of the extractions had upon the HCT-15 colon cancer cells, and concluded that the inhibiting effects upon cells were induced by apoptosis. Result : FUP effectively inhibited the growth of HCT-15 colon cancer cells. After analyzing the DNA fragmentation, the study observed a DNA ladder, while examining the cells, and found an increase of sub-G1 hypodiploid cells. On the changes regarding the nucleus of the cells, a condensation of cells and chromatin, as well as an apoptotic body was clearly observed. By observing through western blot from FUP, the study found a decreased level of Bcl-2 from HCT-15 colon cancer cells, but the increased level of Bax and cleaved caspase-3, which as a result induced apoptosis, inhibiting the growth of HCT-15 colon cancer cells. FUP increased the natural death of HCT-15 colon cancer cells by the induction of apoptosis. FUP seemed to have no suppressing effect upon HL-60/MX2 cells. However, compared to the fucoidan, the study was able to clearly observe morphological changes of HCT-15 cells apoptosis, in a 1/2 concentration. Conclusion : FUP had antiproliferative effects on different kinds of cancer cells, while proving especially efficacious against colon cancer cells.

Keywords

References

  1. Lee KS, An DK, Shin MK, Kim CM. Wanyeok-Jungyakdaesajeon. Seoul : Jungdam publisher. 1997 : 393-7, 4650-2, 6076-82.
  2. Kang JW. Haesansikmul-Hak. Seoul : Daehangyogwaseo Co Ltd. 1984 : 214-7.
  3. State Administration of Traditional chiense medicine of the People's Republic of China. Zhonghuabencao. Vol. 10. Shanghai : Shanghai Scientific and Technical Publishers. 1999 : 453-8.
  4. Lee YJ. A Study on Mineral and Alginic acid Contents by Different Parts of Sea Mustards(Undaria pinnatifida). Kor J Food Culture. 2004 ; 19(6) : 691-700.
  5. Koo JG, Jo KS, Do JR, Woo SJ. Isolation and purification of fucoidans from Laminaria religiosa and Undaria pinnatifida in Korea. J Kor Fish Soc. 1995 ; 28(2) : 227-36.
  6. Pettitt TR, Jones AL, Harwood JL. Lipids of the marine red algae, chondrus crispus and polysiphonia lanosa. Phytochemistry. 1989 ; 28 : 399-408. https://doi.org/10.1016/0031-9422(89)80020-2
  7. Hong JS, Kwon YJ, Kim YH, Kim MK, Park IW, Kang KH. Fatty acid composition of Miyeok(Undaria pinnatifida) and Pare(Enteromorpha compressa). J Kor Soc Food Nutr. 1991 ; 20(4) : 376-80.
  8. Kim JS, Ryo BH, Park DW, Ryo GW. For the prevention and treatment of tumors diet. J Kor Oriental Oncology. 1995 ; 1(1) : 231-48.
  9. Lee JJ, Song YK, Lim HH. Fucoidan extract from Laminaria religiosa suppresses ischemia-induced apoptosis and cell proliferation in the hippocampus of gerbils. Kor J Oriental Medicine. 2006 ; 27(4) : 105-15.
  10. Seoul National University College of Medicine. Jongyang-Hak. Seoul: Seoul National University publisher. 1989 : 1-7.
  11. Nieves-Neira W, Pommier Y. Apoptotic response to camptothecin and 7-hydroxystaurosporine (UCN-01) in the 8 human breast cancer cell lines of the NCI Anticancer Drug Screen: multifactorial relationships with topo isomerase I, protein kinase C, Bcl-2, p53, MDM-2 and caspase pathways. Int J Cancer. 1999 ; 82(3) : 396-404. https://doi.org/10.1002/(SICI)1097-0215(19990730)82:3<396::AID-IJC13>3.0.CO;2-Z
  12. Piazza GA, Rahm AL, Krutzsch M, Sperl G, Paranka NS, Gross PH, Brendel K, Burt RW, Alberts DS, Pamukcu R et al. Antineoplastic drugs sulindac sulfide and sulfone inhibit cell growth by inducing apoptosis. Cancer Res. 1995 ; 55(14) : 3110-16.
  13. Mervyn J, Mary D, Gerald MC. Antitumor activity of 1-naphthol against L1210 leukemia in vivo and enrlich ascites tumor cells in vivo and in vitro. Cancer letters. 1986 ; 33(3) : 347-54. https://doi.org/10.1016/0304-3835(86)90075-3
  14. Lee CH. Studies on the Inhibitory Effect of Berberidis Ramulus to the Liver and Colorectal Ramulus. Graduate School of Kyungwoon University. 2007.
  15. Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. Evaluation of a tetrazolium-based semiautomated colormetric assay. Cancer Res. 1987 ; 47(4) : 943-6.
  16. Nicoletti I, Migliorati C, Pagliacci MC, Grignani F, Riccardi C. A rapid and simple method for measuring thymocyte apoptosis by propidium iodide staining and flow cytometry. J Immunol Methods. 1991 ; 139(2) : 271-9. https://doi.org/10.1016/0022-1759(91)90198-O
  17. Luo Y, Kessel D. Initiation of apoptosis versus necrosis by photodynamic therapy with chloroaluminum phthalocyanine. Photochem Photobiol. 1997 ; 66(4) : 479-83. https://doi.org/10.1111/j.1751-1097.1997.tb03176.x
  18. Tao HJ. Mingyibielu. Beijing : Renminweisheng publisher. 1986 : 157.
  19. Lee SS. Bonchokangmok. Seoul : Daeseongmoonhwasa. 1995 : 396-8.
  20. Cho JK. Hanbanglimsangjongyanghak. Daejeon : Joomin publisher. 2005 : 753.
  21. Kim HS, Bae TJ. Studies on the Hydrolysis of Seaweed Using Microorganisms and Its Application -I. Screening of Microfloras Involved in Hydrolysis of Sea Tangle (Laminaria japonica) and Sea Mustard (Undaria pinnatifida). J Kor Fish Soc. 2002 ; 35(4) : 438-44. https://doi.org/10.5657/kfas.2002.35.4.438
  22. Duarte MER, Cardoso MA, Noseda MD, Cerezo AS. Structural studies on fucoidans from the brown seaweed Sargassum stenophyllum. Carbohydr Res. 2001 ; 333(4) : 281-93. https://doi.org/10.1016/S0008-6215(01)00149-5
  23. Evan G, and Littlewood T. A matter of life and cell death. Science. 1998 ; 281(5381) : 1317-22. https://doi.org/10.1126/science.281.5381.1317
  24. Piao W, Yoo J, Lee DK, Hwang HJ, Kim JH. Induction of G(2)/Mphase arrest and apoptosis by a new synthetic anti-cancer agent, DW2282, in promyelocytic leukemia (HL-60) cells. Biochem Pharmacol. 2001 ; 62(11) : 1439-47. https://doi.org/10.1016/S0006-2952(01)00796-1
  25. Antonsson B, Martinou JC. The Bcl-2 protein family. Exp Cell Res. 2000 ; 256(1) : 50-7. https://doi.org/10.1006/excr.2000.4839
  26. Riedl SJ, Shi Y. Molecular mechanisms of caspase regulation during apoptosis. Nat Rev Mol Cell Biol. 2004 ; 5(11) : 897-907. https://doi.org/10.1038/nrm1496