Reproductive and Developmental Biology

  • 제37권2호
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  • Pages.57-64
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  • 2013
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  • 1738-2432(pISSN)
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  • 2288-0151(eISSN)
한국동물번식학회 (The Korean Society of Animal Reproduction)
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Effects of Trichostatin A on In Vitro Development of Porcine Parthenogenetic and Nuclear Transfer Embryos

  • Diao, Yun-Fei (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Kenji, Naruse (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Han, Rong-Xun (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Lin, Tao (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Oqani, Reza-K. (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Kang, Jung-Won (Research Center for Transgenic and Cloned Pigs, Chungnam National University) ;
  • Jin, Dong-Il (Research Center for Transgenic and Cloned Pigs, Chungnam National University)
  • 투고 : 2013.05.10
  • 심사 : 2013.06.08
  • 발행 : 2013.06.30
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초록

Developmental potential of cloned embryos is related closely to epigenetic modification of somatic cell genome. The present study was to investigate the effects of applying histone deacetylation inhibitor, trichostatin A (TSA) to activated porcine embryos on subsequent development of porcine parthenogenetic and nuclear transfer embryos. Electrically activated oocytes were treated with 5 nM TSA for different exposure times (0, 1, 2 and 4 hr) and then the activated embryos were cultured for 7 days. The reconstructed embryos were treated with different concentrations of 0, 5, 10 and 25 nM TSA for 1 hr. Also 5 nM TSA was tested with different exposure times of 0, 0.5, 1, 2 and 4 hr. And fetal fibroblast cells were treated with 50 nM TSA for 1, 2 or 4 hr and with 5 nM TSA for 1 hr. Cumulus-free oocytes were enucleated and reconstructed by TSA-treated donor cells and electrically fused and cultured for 6 days. In parthenogenetic activation experiments, 5 nM TSA treatment for 1 hr significantly improved the percentage of blastocyst developmental rates than the other groups. Total cell number of blastocysts in 1 hr group was significantly higher than other groups or control. Similarly, blastocyst developmental rates of porcine NT embryos following 5 nM TSA treatment for 1 hr were highest. And the reconstructed embryos from donor cells treated by 50 nM TSA for 1 hr improved the percentage of blastocyst developmental rates than the control group. In conclusion, TSA treatment could improve the subsequent blastocyst development of porcine parthenogenetic and nuclear transfer embryos.

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