Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Medicinal Plant Water Extracts on Expression of Anti-diabetic Enzymes mRNA

약용식물 물 추출물이 항당뇨 효소의 유전자 발현에 미치는 영향

  • Kim, Hyun Sook (Well-being Bioproducts RIC Center, Kangwon National University) ;
  • Kim, Tae Woo (Well-being Bioproducts RIC Center, Kangwon National University) ;
  • Kim, Dae Jung (Well-being Bioproducts RIC Center, Kangwon National University) ;
  • Kim, Kyoung Kon (Dept. of Bio-Health Technology, Kangwon National University) ;
  • Choe, Myeon (Well-being Bioproducts RIC Center, Kangwon National University)
  • 김현숙 (강원대학교 웰빙특산물산업화지역혁신센터) ;
  • 김태우 (강원대학교 웰빙특산물산업화지역혁신센터) ;
  • 김대중 (강원대학교 웰빙특산물산업화지역혁신센터) ;
  • 김경곤 (강원대학교 생명건강공학과) ;
  • 최면 (강원대학교 웰빙특산물산업화지역혁신센터)
  • Received : 2013.03.08
  • Accepted : 2013.04.02
  • Published : 2013.07.31
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Abstract

This study evaluated the anti-diabetic effects of medicinal plant water extracts on expression of hepatic glucokinase (GCK), pyruvate dehydrogenase (PDH) and acetyl-CoA carboxylase (ACC) mRNA. GCK, PDH and ACC mRNA expression levels were measured by RT-PCR. The medicinal plants used in our study were Cordyceps militaris (CM), Perilla sikokiana (PS), Salvia miltiorrhiza Bunge (SMB), Panax notoginseng (PN) and Angelica utilis Makino (AUM). We found that GCK mRNA expression was increased to about 181% at the 250 ppm of CM water extract. Furthermore, we also found that CM and AUM water extracts stimulated PDH mRNA expression level related to glucose metabolism, however, PS, SMB and PN did not stimulate PDH mRNA expression as expected. Expression of ACC mRNA was also significantly higher in both CM and AUM water extracts. Overall, the results of our study suggest that CM and AUM water extracts stimulate expression of hepatic GCK, PDH and ACC mRNA.

본 연구에서는 동충하초, 자소엽, 단삼, 전칠, 명월초 물 추출물이 당대사 관련 효소인 GCK(glucokinase), PDH(pyruvate dehydrogenase) 및 ACC(acetyl CoA carboxylase) mRNA 발현 정도에 미치는 영향을 검토하였다. GCK mRNA 발현은 동충하초 물 추출물을 250 ppm로 처리했을때 181%로 가장 높게 증가되었다. PDH mRNA 발현은 100 ppm에서 명월초(215%)> 단삼(193%)> 전칠(125%)> 동충하초(111%)의 순으로 증가되었으며, 250 및 500 ppm에서는 각각 명월초(251%)와 동충하초(210%) 물 추출물에서 높게 나타났다. ACC mRNA 발현은 자소엽, 단삼, 전칠의 경우 대조구보다 낮거나 유사한 수준을 나타내었지만, 동충하초 물 추출물은 대조구와 비교하여 100 ppm에서 141%, 250 ppm에서 284%로 크게 증가되었다. 명월초 물 추출물은 187~199% 범위에서 ACC mRNA 발현을 크게 증가시켰다. 결과적으로 동충하초와 명월초 물 추출물이 다른 소재들에 비해 간의 GCK, PDH, ACC mRNA 발현을 증가시킴으로써 혈당강하 작용을 나타낼 것으로 판단하였다.

Keywords

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