Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Selective or Class-wide Mass Fingerprinting of Phosphatidylcholines and Cerebrosides from Lipid Mixtures by MALDI Mass Spectrometry

  • Lee, Gwangbin (Department of Chemistry, Hankuk University of Foreign Studies) ;
  • Son, Jeongjin (Department of Chemistry, Hankuk University of Foreign Studies) ;
  • Cha, Sangwon (Department of Chemistry, Hankuk University of Foreign Studies)
  • Received : 2013.04.08
  • Accepted : 2013.04.23
  • Published : 2013.07.20
Download PDF
⟨ Previous Next ⟩

Abstract

Matrix assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is a very effective method for lipid mass fingerprinting. However, MALDI MS suffered from spectral complexities, differential ionization efficiencies, and poor reproducibility when analyzing complex lipid mixtures without prior separation steps. Here, we aimed to find optimal MALDI sample preparation methods which enable selective or class-wide mass fingerprinting of two totally different lipid classes. In order to achieve this, various matrices with additives were tested against the mixture of phosphatidylcholine (PC) and cerebrosides (Cers) which are abundant in animal brain tissues and also of great interests in disease biology. Our results showed that, from complex lipid mixtures, 2,4,6-trihydroxyacetophenone (THAP) with $NaNO_3$ was a useful MALDI matrix for the class-wide fingerprinting of PC and Cers. In contrast, THAP efficiently generated PC-focused profiles and graphene oxide (GO) with $NaNO_3$ provided Cer-only profiles with reduced spectral complexity.

Keywords

References

  1. Fuchs, B.; Suss, R.; Schiller, J. Prog. Lipid Res. 2010, 49, 450. https://doi.org/10.1016/j.plipres.2010.07.001
  2. Cha, S. W.; Yeung, E. S. Anal. Chem. 2007, 79, 2373. https://doi.org/10.1021/ac062251h
  3. Estrada, R.; Yappert, M. C. J. Mass Spectrom. 2004, 39, 412. https://doi.org/10.1002/jms.603
  4. Stubiger, G.; Pittenauer, E.; Belgacem, O.; Rehulka, P.; Widhalm, K.; Allmaier, G. Rapid Commun. Mass Spectrom. 2009, 23, 2711. https://doi.org/10.1002/rcm.4173
  5. Cerruti, C. D.; Touboul, D.; Guerineau, V.; Petit, V. W.; Laprevote, O.; Brunelle, A. Anal. Bioanal. Chem. 2011, 401, 75. https://doi.org/10.1007/s00216-011-4814-9
  6. Sugiura, Y.; Setou, M. Rapid Commun. Mass Spectrom. 2009, 23, 3269. https://doi.org/10.1002/rcm.4242
  7. Griffiths, R. L.; Bunch, J. Rapid Commun. Mass Spectrom. 2012, 26, 1557. https://doi.org/10.1002/rcm.6258
  8. Weng, M. F.; Chen, Y. C. Rapid Commun. Mass Spectrom. 2004, 18, 1421. https://doi.org/10.1002/rcm.1501
  9. Sun, G.; Yang, K.; Zhao, Z.; Guan, S.; Han, X.; Gross, R. W. Anal. Chem. 2008, 80, 7576. https://doi.org/10.1021/ac801200w
  10. Jackson, S. N.; Ugarov, M.; Egan, T.; Post, J. D.; Langlais, D.; Schultz, J. A.; Woods, A. S. J. Mass Spectrom. 2007, 42, 1093. https://doi.org/10.1002/jms.1245
  11. Niedermeyer, T. H. J.; Strohalm, M. Plos One 2012, 7.
  12. Stubiger, G.; Belgacem, O. Anal. Chem. 2007, 79, 3206. https://doi.org/10.1021/ac062236c
  13. Kim, Y. K.; Na, H. K.; Kwack, S. J.; Ryoo, S. R.; Lee, Y.; Hong, S.; Hong, S.; Jeong, Y.; Min, D. H. Acs Nano 2011, 5, 4550. https://doi.org/10.1021/nn200245v
  14. Zhou, X. Z.; Wei, Y. Y.; He, Q. Y.; Boey, F.; Zhang, Q. C.; Zhang, H. Chem. Commun. 2010, 46, 6974. https://doi.org/10.1039/c0cc01681k
  15. Howard, K. L.; Boyer, G. L. Rapid Commun. Mass Spectrom. 2007, 21, 699. https://doi.org/10.1002/rcm.2887
  16. Liu, H.; Du, Z.; Wang, J.; Yang, R. Appl. Environ. Microbiol. 2007, 73, 1899. https://doi.org/10.1128/AEM.02391-06
  17. Han, X. L.; Cheng, H. J. Lipid Res. 2005, 46, 163.

Cited by

  1. Graphene nanoflakes as an efficient ionizing matrix for MALDI-MS based lipidomics of cancer cells and cancer stem cells vol.2, pp.42, 2014, https://doi.org/10.1039/C4TB00970C
  2. Direct Analysis of Triacylglycerols from Crude Lipid Mixtures by Gold Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry vol.25, pp.5, 2014, https://doi.org/10.1007/s13361-014-0844-9
  3. Fractional Factorial Design of MALDI-TOF-MS Sample Preparations for the Optimized Detection of Phospholipids and Acylglycerols vol.88, pp.12, 2016, https://doi.org/10.1021/acs.analchem.6b00512
  4. Analysis of Lipids in Deciduous Teeth by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry (MALDI MS) vol.8, pp.4, 2013, https://doi.org/10.5478/msl.2017.8.4.105