Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Inhibition of Metastatic Lung Cancer in C57BL/6 Mice by Marine Mangrove Rhizophora apiculata

  • Prabhu, V. Vinod (Department of Biotechnology, Karunya University) ;
  • Guruvayoorappan, C. (Department of Biotechnology, Karunya University)
  • Published : 2013.03.30
Download PDF
⟨ Previous Next ⟩

Abstract

Metastasis is one of the hallmarks of malignant neoplasms and is the leading cause of death in many cancer patients. A major challenge in cancer treatment is to find better ways to specifically target tumor metastasis. In this study, the anti-metastatic potential of the methanolic extract of Rhizophora apiculata (R.apiculata) was evaluated using the B16F-10 melanoma induced lung metastasis model in C57BL/6 mice. Metastasis was induced in C57BL/6 mice by injecting highly metastatic B16F-10 melanoma cells through the lateral tail vein. Simultaneous treatment with R.apiculata extract (10 mg/kg b.wt (intraperitoneal) significantly (p<0.01) inhibited pulmonary tumor nodule formation (41.1 %) and also increased the life span (survival rate) 107.3 % of metastatic tumor bearing animals. The administration of R.apiculata extract significantly (p<0.01) reduced biochemical parameters such as lung collagen hydroxyproline, hexosamine, uronic acid content, serum nitric oxide (NO), ${\gamma}$-glutamyl transpeptidase (GGT) and sialic acid levels when compared to metastasis controls. These results correlated with lung histopathology analysis of R.apiculata extract treated mice showing reduction in lung metastasis and tumor masses. Taken together, our findings support that R.apiculata extract could be used as a potential anti-metastasis agent against lung cancer.

Keywords

References

  1. Ambs S, Merriam WG, Bennett WP, et al (1998). Frequent nitric oxide synthase-2 expression in human colon adenomas: implication for tumor angiogenesis and colon cancer progressionm. Cancer Res, 58, 334-41.
  2. Antony JJ, Sivalingam P, Siva D, et al (2011). Comparative evaluation of antibacterial activity of silver nanoparticles synthesized using Rhizophora apiculata and glucose. Colloids Surf B Biointerfaces, 88, 134-40. https://doi.org/10.1016/j.colsurfb.2011.06.022
  3. Aragon-Ching JB, Zujewski J (2007). CNS metastasis: an old problem in a new guise. Clinical Cancer Res, 13, 1644-7. https://doi.org/10.1158/1078-0432.CCR-07-0096
  4. Bergman I, Loxley R (1970). The determination of hydroxyproline in urine hydrosylates. Clin. Chem Acta, 27, 347-9. https://doi.org/10.1016/0009-8981(70)90355-4
  5. Bhatia S, Tykodll SS, Thompson JA (2009). Treatment of metastatic melanoma: an overview. Oncology (Williston Park), 23, 488-96.
  6. Bhavanandhan VP, Katlic AW, Banks J, et al (1981). Partial characterization of sialoglycopeptides produced by cultured human melanoma cells and melanocytes. Biochemistry, 20, 3586-94. https://doi.org/10.1021/bi00515a043
  7. Bitter T, Muir HM (1962). A modified uronic acid carbazole reaction. Anal. Biochem, 4, 330-34.
  8. Branco-Price C, Zhang N, Schnelle M, et al (2012). Endothelial cell HIF-$1{\alpha}$ and HIF-$2{\alpha}$ differentially regulate metastatic success. Cancer Cell, 21, 52-65. https://doi.org/10.1016/j.ccr.2011.11.017
  9. Carretero J, Obrador E, Anasagasti MJ, et al (1999). Growthassociated changes in glutathione content correlate with liver metastatic activity of B16 melanoma cells. Clin Exp Metastasis, 17, 567-74. https://doi.org/10.1023/A:1006725226078
  10. Chambers AF, Groom AC, MacDonald IC (2002). Dissemination and growth of cancer cells in metastatic sites. Nat Rev Cancer, 2, 563-72. https://doi.org/10.1038/nrc865
  11. Cole SP (1986). Rapid chemosensitivity testing of human lung tumor cells using the MTT assay. Cancer Chemother Pharmacol, 17, 259-63.
  12. Edwards P, Cendan JC, Topping DB, et al (1933). A colourimetric method for determination of glucosamine. J Biochem, 27, 1824-8.
  13. Edwards P, Cendan JC, Topping DB, et al (1996). Tumor cell nitric oxide inhibits cell growth in vitro, but stimulates tumorigenesis and experimental lung metastasis in vivo. J Surg Res, 63, 49-52. https://doi.org/10.1006/jsre.1996.0221
  14. Engbrin JA, Hoffman MP, Karmand AJ, Kleinman HK (2002). The B16F-10 receptor for a metastasis promoting site on Laminin-1 is a heparan sulfate/ chondroitin sulfate containing proteoglycan. Cancer Res, 62, 3549-54.
  15. Fukumura D, Kashiwagi S, Jain RK (2006). The role of nitric oxide in tumor progression. Nat Rev Cancer, 6, 521-34. https://doi.org/10.1038/nrc1910
  16. Fuster MM, Esko JD (2005). The sweet and sour of cancer:Glycans as novel therapeutic targets. Nature Reviews Cancer, 5, 526-42. https://doi.org/10.1038/nrc1649
  17. Green LC, Wagner DA, Glogowski J, et al (1982). Analysis of nitrate, nitrite and (15N) nitrate in biological fluids. Anal Biochem, 126, 131-8. https://doi.org/10.1016/0003-2697(82)90118-X
  18. Gupta GP, Massague J (2002). Cancer metastasis: building a framework. Cell, 127, 679-95.
  19. Herzig M, Christofori G (2002). Recent advances in cancer research: mouse models of tumorigenesis. Biochim Biophys Acta, 1602, 97-113.
  20. Hunter KW, Crawford NPS, Alsarraj J (2008). Mechanisms of metastasis. Breast Cancer Res, 10, 2.
  21. Hyoudou K, Nishikawa M, Umeyama Y, et al (2004). Inhibition of metastatic tumor growth in mouse lung by repeated administration of polyethylene glycol-conjugated catalase: quantitative analysis with firefly luciferase-expressing melanoma cells. Clin Cancer Res, 15, 7685-91.
  22. Khan N, Mukhtar H (2010). Cancer and metastasis: prevention and treatment by green tea. Cancer Metastasis Rev, 29, 435-45. https://doi.org/10.1007/s10555-010-9236-1
  23. Klein CA (2008). Cancer. The metastasis cascade. Science 321,1785-87. https://doi.org/10.1126/science.1164853
  24. Kuttan R, Bhanumathy P, Nirmala K, George MC (1985). Possible anticancer activity of turmeric. Nutr Cancer, 29, 197-202.
  25. Lee JM, Yanagawa J, Peebles KA, et al (2008). Inflammation in lung carcinogenesis: new targets for lung cancer chemoprevention and treatment. Crit Rev Oncol Hematol, 66, 208-17. https://doi.org/10.1016/j.critrevonc.2008.01.004
  26. Leyon PV, Kuttan G (2004). Effect of Withania somnifera on B16F-10 meanoma induced metastasis in mice. Phytother Res, 18, 118-22. https://doi.org/10.1002/ptr.1378
  27. Leyon PV, Lini CC, Kuttan G (2005). Inhibitory effect of Boerhaavia diffusa on experimental metastasis by B16F10 melanoma in C57BL/6 mice. Lifesciences, 76, 133-49.
  28. Lipponen P, Aaltomaa S, Tammi R, Argen U, Kosma VM (2001). High stromal hyaluronan level is associated with poor differentiation and metastases in prostate cancer. Eur J Cancer, 37, 849-56. https://doi.org/10.1016/S0959-8049(00)00448-2
  29. Luzzi KJ, MacDonald IC, Schmidt EE, et al (1998). Multistep nature of metastatic inefficiency: dormancy of solitary cells after successful extravasation and limited survival of early micrometastases. Am J Pathol, 153, 865-73. https://doi.org/10.1016/S0002-9440(10)65628-3
  30. Mokkhasmit M, Swatdimongkol. K, Satrawaha P (1971). Study on toxicity of Thai medicinal plants. Bull Dept Med Sci. 12, 36-65.
  31. Nathan C (1992). Nitric oxide as a secretory product of mammalian cells. FASEB J, 6, 3051-64.
  32. Nelson DL, Cox MM (2005). Lehninger's Principles of Biochemistry, 4th Edition, W. H. Freeman and Company, New York.
  33. Nguyen DX, Massagué J (2007). Genetic determinants of cancer metastasis. Nat Rev Genet, 8, 341-52.
  34. Nonaka Y, Iwagaki H, Kimura T, Fuchimoto S, Orita K (1993). Effect of reactive oxygen intermediates on the in vitro invasive capacity of tumor cells and liver metastasis in mice. Int J Cancer, 54, 983-86. https://doi.org/10.1002/ijc.2910540620
  35. Prabhu VV, Guruvayoorappan C (2010). Nitric oxide: Pros and cons in tumor progression. Immunopharmacol Immunotoxicol, 32, 387-92. https://doi.org/10.3109/08923970903440192
  36. Prabhu VV, Guruvayoorappan C (2012). Evaluation of immunostimulant activity and chemoprotective effect of mangrove Rhizophora apiculata against cyclophosphamide induced toxicity in BALB/c mice. Immunopharmacol Immunotoxicol, 34, 607-14.
  37. Prabhu VV, Guruvayoorappan C (2012a). Anti-inflammatory and anti-tumor activity of the marine mangrove Rhizophora apiculata. J Immunotoxicology, 9, 341-352. https://doi.org/10.3109/1547691X.2012.660997
  38. Prabhu VV, Siddikuzzaman, Grace VM, Guruvayoorappan C (2012b). Targeting tumor metastasis by regulating Nm23 gene expression. Asian Pac J Cancer Prev, 13, 3539-48. https://doi.org/10.7314/APJCP.2012.13.8.3539
  39. Pradeep K (2002). Effect of piperine on the inhibition of lung metastasis induced B16F-10 melanoma cells in mice. Clinical & Experimental Metastasis, 19, 703-08. https://doi.org/10.1023/A:1021398601388
  40. Premanathan M, Arakaki R, Izumi H, et al (1999). Antiviral properties of a mangrove plant, Rhizophora apiculata Blume, against human immunodeficiency virus. Antiviral Res, 44, 113-22. https://doi.org/10.1016/S0166-3542(99)00058-3
  41. Schantz SP, Brown BW, Lira E, Taylor SL, Beddingfield N (1987). Evidence for the role of natural immunity in the control of metastatic spread of head and neck cancer. Cancer Immunology Immunotherapy, 25, 141-48.
  42. Schauer R (1985). Sialic acids and their role as biological masks. Trends Biochem Sci, 10, 357-60. https://doi.org/10.1016/0968-0004(85)90112-4
  43. Shi Q, Huang S, Jiang W, et al (1999). Direct correlation between nitric oxide synthase II inducibility and metastatic ability of UV-2237 murine fibrosarcoma cells carrying mutant p53. Cancer Res, 59 , 2072-75.
  44. Sithranga BN, Kandasamy K, Subramanian M, You-Jin J (2011). Effect of mangrove tea extract from ceriops decandra (Griff.) Ding Hou. on salivary bacterial flora of DMBA Induced hamster buccal pouch carcinoma. Indian J Microbiol, 51, 338-44. https://doi.org/10.1007/s12088-011-0096-3
  45. Skoza L, Mohos S (1976). Stable thiobarbituric acid chromophore with dimethyl sulphoxide. Application to sialic acid assay in analytical de o acetylation. Biochem J, 159, 457-62.
  46. Talmadge JE, Fidler IJ (2010). AACR centennial series: the biology of cancer metastasis: historical perspective. Cancer Res, 70, 5649-69. https://doi.org/10.1158/0008-5472.CAN-10-1040
  47. Tate SS, Meister A (1974). Inhibition of gamma glutamyl transpeptidase amino acids, peptides and derivatives and analogs of glutathione. J Biochem, 23, 1602.
  48. Thejass P, Kuttan, Girija (2006). Antimetastatic activity sulforaphane. Life Sci. 78, 3043-50. https://doi.org/10.1016/j.lfs.2005.12.038
  49. Vedolva E, Borovansky J (1994). Evaluation of serum sialic acid fractions as marker from malignant melanoma. Cancer Lett, 78, 171-5. https://doi.org/10.1016/0304-3835(94)90047-7
  50. Voet D, Voet JG (1995). Biochemistry, New York: John Wiley, 157-258.
  51. Wang YP, Wang MZ, Luo YR, Shen Y, Wei ZX (2012). Lentivirus-mediated shRNA interference targeting SLUG inhibits lung cancer growth and metastasis. Asian Pac J Cancer Prev, 13, 4947-51. https://doi.org/10.7314/APJCP.2012.13.10.4947
  52. West DC, Hampson IN, Arnold F, et al (1985). Angiogenesis induced by degradation products of hyaluronic acid. Science, 228, 1324-26. https://doi.org/10.1126/science.2408340
  53. WHO glonocon report 2008.http://globocan.iarc.fr/factsheets/populations/factsheet.asp?uno=900
  54. WHO Report (2008). National Policy on Traditional Medicine and Regulation of Herbal Medicines - Report of a WHO global survey.

Cited by

  1. Different Immunology Mechanisms of Phellinus igniarius in Inhibiting Growth of Liver Cancer and Melanoma Cells vol.15, pp.8, 2014, https://doi.org/10.7314/APJCP.2014.15.8.3659
  2. : Future Prospects vol.2014, pp.2356-7872, 2014, https://doi.org/10.1155/2014/938543
  3. Plant natural products: from traditional compounds to new emerging drugs in cancer therapy vol.47, pp.6, 2014, https://doi.org/10.1111/cpr.12143
  4. Biomimetic synthesis of silver nanoparticles from Streptomyces atrovirens and their potential anticancer activity against human breast cancer cells vol.11, pp.8, 2017, https://doi.org/10.1049/iet-nbt.2016.0222
  5. Pinus massoniana bark extract inhibits migration of the lung cancer A549 cell line vol.13, pp.2, 2017, https://doi.org/10.3892/ol.2016.5509
  6. Sediment quality, elemental bioaccumulation and antimicrobial properties of mangroves of Indian Sundarban pp.1573-2983, 2018, https://doi.org/10.1007/s10653-018-0145-5