Weed & Turfgrass Science

The Korean Society of Weed Science & The Turfgrass Society of Korea Archive (한국잡초학회 & 한국잔디학회)
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Identification of Streptomyces scopuliridis KR-001 and Its Herbicidal Characteristics

Streptomyces scopuliridis KR-001의 분리 동정 및 잡초 방제효과

  • Lee, Boyoung (Eco - friendly and New Materials Research Group, Korea Research Institute of Chemical Technology) ;
  • Kim, Jae Deok (Eco - friendly and New Materials Research Group, Korea Research Institute of Chemical Technology) ;
  • Kim, Young Sook (Eco - friendly and New Materials Research Group, Korea Research Institute of Chemical Technology) ;
  • Ko, Young Kwan (Eco - friendly and New Materials Research Group, Korea Research Institute of Chemical Technology) ;
  • Yon, Gyu Hwan (Eco - friendly and New Materials Research Group, Korea Research Institute of Chemical Technology) ;
  • Kim, Chang-Jin (Microbial Resource Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Koo, Suk Jin (Korea Research Institute of Bioscience and Biotechnology) ;
  • Choi, Jung Sup (Eco - friendly and New Materials Research Group, Korea Research Institute of Chemical Technology)
  • 이보영 (한국화학연구원 친환경신물질연구그룹) ;
  • 김재덕 (한국화학연구원 친환경신물질연구그룹) ;
  • 김영숙 (한국화학연구원 친환경신물질연구그룹) ;
  • 고영관 (한국화학연구원 친환경신물질연구그룹) ;
  • 연규환 (한국화학연구원 친환경신물질연구그룹) ;
  • 김창진 (한국생명공학연구원 생물자원센터) ;
  • 구석진 ((주)목우연구소) ;
  • 최정섭 (한국화학연구원 친환경신물질연구그룹)
  • Received : 2013.02.22
  • Accepted : 2013.03.11
  • Published : 2013.03.31
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Abstract

With increasing environmental issues from synthetic chemical herbicides, microbe-originated herbicides could be a fascinating alternative in current agriculture. We isolated Streptomyces strains that produced herbicidal active metabolite(s) against a grass weed Digitaria sanguinalis. According to the result from 16S rDNA sequence comparison with the close strains, the best isolate (Code name MS-80673) was identified as Streptomyces scopuliridis KR-001. The closest type strain was Streptomyces scopuliridis RB72 which was previously reported as a bacteriocin producer. The optimal culture condition of S. scopuliridis KR-001 was $28^{\circ}C$, pH 7.0 and culture period 4 to7 days. Both of soil and foliar application of the crude culture broth concentrate was effective on several troublesome or noxious weed species such as a Sciyos angulatus in a greenhouse and field condition. Phytotoxic symptoms of the culture broth concentrate of S. scopuliridis KR-001 by foliar application were wilting and burndown of leaves, and stems followed by discoloration and finally plant death. In crops such as rice, wheat, barley, hot pepper and tomato, growth inhibition was observed. These results suggest that the new S. scopuliridis KR-001 strain producing herbicidal metabolites may be a new bio-herbicide candidate and/or may provide a new lead molecule for a more efficient herbicide.

토양 방선균 유래의 천연 제초활성 후보소재를 발굴하고, 이 후보소재의 온실 및 포장 실증 평가를 통해 보다 친환경적이고 효율적인 잡초 관리를 위한 잡초방제제로의 실용화 가능성을 검토하고자 본 연구를 수행하였다. 3,000여 종의 토양 방선균으로부터 강력하고 독특한 제초활성을 발현하는 후보 방선균 2종을 선발하였으며, 선발된 후보 방선균의 16S rDNA 서열을 분석한 결과 S. scopuliridis RB72와 가장 유사도가 높아 선발 균주를 S. scopuliridis KR-001이라 명명하였다. 후보 방선균의 최적 배양조건을 확립하였고, 온실 및 포장조건에서 주요 문제잡초와 난방제 잡초, 환경 위해 잡초 가시박 방제효과를 평가를 통해 친환경적인 천연물 제초제로서의 개발 가능성을 확인하였다. 한편, 후보 방선균 S. scopuliridis KR-001 배양액은 작물에 대한 선택성은 없었기 때문에 비선택성 경엽처리제로의 개발 가능성을 고려한 처리 농도 및 시기 등에 대한 진전단계 연구를 진행할 필요성이 제기되었다. 향후 배양액 수준에서 제형 연구를 통해 약효증진기술과 대량생산성 기술 개발을 통해 실용화 가능성을 검토하는 한편, 배양액 내의 살초성분에 대한 화학구조 구명을 통해 방선균 유래의 천연 제초제를 개발 할 수 있는 선도물질 발굴을 위한 추가 연구가 필요한 것으로 판단하였다.

Keywords

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