International Journal of Oral Biology

The Korean Academy of Oral Biology (대한구강생물학회)
권호 표지
DOI QR코드

DOI QR Code

Prevalence of Putative Periodontopahogen TM7 and Dialister in Dental Plaque of Koreans

  • Kim, Yeon-Hee (Department of Oral Microbiology, College of Dentistry, Research Institute of Oral Science, Gangneung-Wonju National University) ;
  • Lee, Si-Young (Department of Oral Microbiology, College of Dentistry, Research Institute of Oral Science, Gangneung-Wonju National University)
  • Received : 2013.02.20
  • Accepted : 2013.04.11
  • Published : 2013.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

TM7 is an uncultivated organism which is present in extremely diverse environments. Members of the Dialister genus are difficult to culture as a result of which many of these strains remain uncultivated. It has been suggested that TM7 and Dialister bacteria may belong to a group of suspected periodontal pathogens. In our current study, the presence of the sebacteria in Korean dental plaque samples was assessed using PCR detection methods with specific primers for 16S ribosomal RNA genes. The experimental group included 84 volunteers (35 males and 49 females). Plaque samples were collected from 4 non-adjacent proximal sites of the molar areas of the mandible in each subject and pooled. TM7 was detectable in 56% and the Dialister genus in 27.5% of the volunteers. Both TM7 and Dialister were present in 20.3% of volunteers. We found that 36.9% of the volunteers were negative for both bacteria. Further studies to evaluate the prevalence of these putative pathogenic bacteria in the Korean population are warranted.

Keywords

References

  1. Haffajee AD, Socransky SS. Microbial etiological agents of destructive periodontal diseases. Periodontol 2000. 1994;5:78-111. https://doi.org/10.1111/j.1600-0757.1994.tb00020.x
  2. Moore WE, Moore LV. The bacteria of periodontal diseases. Periodontol 2000. 1994;5:66-77. https://doi.org/10.1111/j.1600-0757.1994.tb00019.x
  3. Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL, Jr. Microbial complexes in subgingival plaque. J Clin Periodontol. 1998;25:134-44. https://doi.org/10.1111/j.1600-051X.1998.tb02419.x
  4. Brinig MM, Lepp PW, Ouverney CC, Armitage GC, Relman DA. Prevalence of bacteria of division TM7 in human subgingival plaque and their association with disease. Appl Environ Microbiol. 2003;69:1687-94. https://doi.org/10.1128/AEM.69.3.1687-1694.2003
  5. Dewhirst FE, Chen T, Izard J, Paster BJ, Tanner AC, Yu WH, Lakshmanan A, Wade WG. The human oral microbiome. J Bacteriol. 2010;192:5002-17. https://doi.org/10.1128/JB.00542-10
  6. Hugenholtz P, Tyson GW, Webb RI, Wagner AM, Blackall LL. Investigation of candidate division TM7, a recently recognized major lineage of the domain bacteria with no known pure-culture representatives. Appl Environ Microbiol. 2001; 67:411-9. https://doi.org/10.1128/AEM.67.1.411-419.2001
  7. Paster BJ, Boches SK, Galvin JL, Ericson RE, Lau CN, Levanos VA, Sahasrabudhe A, Dewhirst FE. Bacterial diversity in human subgingival plaque. J Bacteriol. 2001;183: 3770-83. https://doi.org/10.1128/JB.183.12.3770-3783.2001
  8. Moore LV, Moore WE. Oribaculum catoniae gen. nov., sp. nov.; Catonella morbi gen. nov., sp. nov.; Hallella seregens gen. nov., sp. nov.; Johnsonella ignava gen. nov., sp. nov.; and Dialister pneumosintes gen. nov., comb. nov., nom. rev., anaerobic gram-negative bacilli from the human gingival crevice. Int J Syst Bacteriol. 1994;44:187-92. https://doi.org/10.1099/00207713-44-2-187
  9. Contreras A, Doan N, Chen C, Rusitanonta T, Flynn MJ, Slots J. Importance of Dialister pneumosintes in human periodontitis. Oral Microbiol Immunol. 2000;15:269-72. https://doi.org/10.1034/j.1399-302x.2000.150410.x
  10. Moore WE, Holdeman LV, Smibert RM, Cato EP, Burmeister JA, Palcanis KG, Ranney RR. Bacteriology of experimental gingivitis in children. Infect Immun. 1984; 46:1-6.
  11. Moore WE, Holdeman LV, Smibert RM, Good IJ, Burmeister JA, Palcanis KG, Ranney RR. Bacteriology of experimental gingivitis in young adult humans. Infect Immun. 1982;38:651-67.
  12. Moore LV, Moore WE, Cato EP, Smibert RM, Burmeister JA, Best AM, Ranney RR. Bacteriology of human gingivitis. J Dent Res. 1987;66:989-95. https://doi.org/10.1177/00220345870660052401
  13. Rocas IN, Siqueira JF,Jr. Characterization of Dialister species in infected root canals. J Endod. 2006;32:1057-61. https://doi.org/10.1016/j.joen.2006.04.010
  14. Doan N, Contreras A, Flynn J, Slots J, Chen C. Molecular identification of Dialister pneumosintes in subgingival plaque of humans. J Clin Microbiol. 2000;38:3043-7.
  15. Socransky SS, Haffajee AD. Periodontal microbial ecology. Periodontol 2000. 2005;38:135-87. https://doi.org/10.1111/j.1600-0757.2005.00107.x
  16. Paster BJ, Russell MK, Alpagot T, Lee AM, Boches SK, Galvin JL, Dewhirst FE. Bacterial diversity in necrotizing ulcerative periodontitis in HIV-positive subjects. Ann Periodontol. 2002;7:8-16. https://doi.org/10.1902/annals.2002.7.1.8
  17. Ferraro CT, Gornic C, Barbosa AS, Peixoto RJ, Colombo AP. Detection of Dialister pneumosintes in the subgingival biofilm of subjects with periodontal disease. Anaerobe. 2007;13:244-8. https://doi.org/10.1016/j.anaerobe.2007.09.002
  18. Ghayoumi N, Chen C, Slots J. Dialister pneumosintes, a new putative periodontal pathogen. J Periodontal Res. 2002;37:75-8. https://doi.org/10.1034/j.1600-0765.2002.05019.x
  19. Siqueira JF, Rocas IN. Positive and negative bacterial associations involving Dialister pneumosintes in primary endodontic infections. J Endod. 2003;29:438-41. https://doi.org/10.1097/00004770-200307000-00003
  20. Siqueira JF,Jr, Rocas IN. Simultaneous detection of Dialister pneumosintes and Filifactor alocis in endodontic infections by 16S rDNA-directed multiplex PCR. J Endod. 2004; 30:851-4. https://doi.org/10.1097/01.DON.0000132300.13023.5D
  21. Siqueira JF, Jung IY, Rocas IN, Lee CY. Differences in prevalence of selected bacterial species in primary endodontic infections from two distinct geographic locations. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2005; 99:641-7. https://doi.org/10.1016/j.tripleo.2004.07.009
  22. Kumar PS, Griffen AL, Barton JA, Paster BJ, Moeschberger ML, Leys EJ. New bacterial species associated with chronic periodontitis. J Dent Res. 2003;82:338-44. https://doi.org/10.1177/154405910308200503