Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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The Effect of Phaseoli Semen Herbal-acupuncture at $KI_{10}$ in Lipopolysaccharide Induced Acute Nephritis in Rats

음곡에 시행한 적소두약침이 LPS로 유도된 흰쥐의 급성 신장염에 미치는 영향

  • Kwak, Kyu In (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Daejeon University) ;
  • Kang, Jae Hui (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Daejeon University) ;
  • Lee, Hyun (Department of Acupuncture & Moxibustion Medicine, College of Oriental Medicine, Daejeon University)
  • 곽규인 (대전대학교 한의과대학 침구의학교실) ;
  • 강재희 (대전대학교 한의과대학 침구의학교실) ;
  • 이현 (대전대학교 한의과대학 침구의학교실)
  • Received : 2013.05.09
  • Accepted : 2013.06.03
  • Published : 2013.06.20
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Abstract

Objectives : This study was designed to evaluate the effects of Phaseoli Semen Herbal-acupuncture(PS-HA) at $KI_{10}$ in acute nephritis induced by lipopolysaccharide(LPS) in rat. Methods : The rats were divided into 5 groups, which were control, LPS, PS-HA, NP and saline group. LPS, PS-HA, NP and saline groups were given LPS to induce acute nephritis and control group did not receive LPS. LPS group did not receive any treatment after the onset of acute nephritis. PS-HA, NP and saline group received PS-HA, normal acupuncture, and saline injection at $KI_{10}$ three times per week, respectively. To evaluate the effect of PS-HA at $KI_{10}$, the complete blood count, BUN, creatinine, TNF-${\alpha}$, and CINC-1 in serum were measured. To show its effect on renal function, creatinine, and total protein in urine was measured as well as urine output. The level of myeloperoxidase in renal tissue was quantified and complete histology was done in kidney samples obtained from the rats. Results : PS-HA group showed a significant reduction in the proportion of WBC and neutrophil, serum BUN, TNF-${\alpha}$, and CINC-1 compared to LPS group. Furthermore, a significant increase in urine output and a decrease in urinary creatinine level, MPO in renal tissue, and number of neutrophils at glomerulus was observed in PS-HA group compared to LPS group Conclusions : PS-HA at $KI_{10}$ was shown to have a significantly effect on treating LPS induced acute nephrits. Therefore, future study is needed to further evaluate the clinical usefulness of PS-HA at $KI_{10}$ in treating acute nephritis.

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References

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