Journal of Acupuncture Research

  • 제30권3호
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  • Pages.1-13
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  • 2013
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  • 2586-288X(pISSN)
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  • 2586-2898(eISSN)
대한침구의학회 (Korean Acupuncture & Moxibustion Medicine Society)
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The Effect of Acupuncture in Promoting Neurogenesis and Angiogenesis after Middle Cerebral Artery Occlusion in Rats

  • Lee, Hong Min (Department of Oriental Medicine, Graduate School, Kyung Hee University) ;
  • Nam, Sang Soo (Department of Oriental Medicine, Graduate School, Kyung Hee University) ;
  • Kim, Yong Suk (Department of Oriental Medicine, Graduate School, Kyung Hee University)
  • 투고 : 2013.04.12
  • 심사 : 2013.05.23
  • 발행 : 2013.06.20
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초록

Objectives : This study was performed to choose more effective neuro-protective acupuncture point and to verify the effect of acupuncture in promoting neurogenesis and angiogenesis as a result of its neuro-vasculo-regenerative effect in middle cerebral artery occlusion model in rats. Methods : By TTc staining we chose the most effective acupuncture point with neuro-protection. We randomly divided into four groups: Such as (1) sham group(with sham-operation), (2) sham+acupuncture group(with sham-operation), (3) middle cerebral artery occlusion group, (4) MCAO+AT group. Acupuncture procedure was performed for four days. Total RNA was extracted using TRIzol reagent, according to the manufacturer's instructions, and was purified using an RNAeasy mini kit. Immuno-histochemistry was performed using primary antibody mouse anti-BrdU, NeuN, Dcx, and VEGF. Results : We found that $ST_{36}$ had the more neuroprotective effect than $LI_{11}$ and $SP_3$. The microarray analysis revealed that 54 genes were more expressed neurogenesis pathway in MCAO+AT group compared with MCAO group(fold changes greater than or equal to twofold change). 11 genes were more expressed angiogenesis pathway. And 7 genes were more expressed VEGF pathway. Immuno-histochemistry revealed that cell proliferation, cell migration and cell maturation were increased. Conclusions : This study demonstrated that acupuncture on $ST_{36}$ had neuro-protective and neuro-restorative effect in ischemic brain injuries. And its mechanism might be related to promote neurogenesis and angiogenesis. These results suggest that acupuncture have potential benefits for the treatment of ischemic stroke.

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참고문헌

  1. Yamashita T, Ninomiya M, Hernandez Acosta P et al. Subventricular zone-derived neuroblasts migrate and differentiate into mature neurons in the post-stroke adult striatum. J Neurosci. 2006 ; 26 : 6627-36. https://doi.org/10.1523/JNEUROSCI.0149-06.2006
  2. Thored P, Arvidsson A, Cacci E et al. Persistent production of neurons from adult brain stem cells during recovery after stroke. Stem Cells. 2006 ; 24 : 739-47. https://doi.org/10.1634/stemcells.2005-0281
  3. Zhang RL, Zhang ZG, Wang L et al. Activated neural stem cells contribute to strokeinduced neurogenesis and neuroblast migration toward the infarct boundary in adult rats. J Cereb Blood Flow Metab. 2004 ; 24 : 441-8.
  4. Thored P, Wood J, Arvidsson A, Cammenga J, Kokaia Z, Lindvall O. Long-term neuroblast migration along blood vessels in an area with transient angiogenesis and increased vascularization after stroke. Stroke. 2007 ; 38 : 3032-9. https://doi.org/10.1161/STROKEAHA.107.488445
  5. Ohab JJ, Fleming S, Blesch A, Carmichael ST. A neurovascular niche for neurogenesis after stroke. The journal of neuroscience. 2006 ; 26(50) : 13007-16. https://doi.org/10.1523/JNEUROSCI.4323-06.2006
  6. Zhang ZG, Chopp M. Vascular endothelial growth factor and angiopoietins in focal cerebral ischemia. Trends Cardiovasc. 2002 ; 12 : 62-6. https://doi.org/10.1016/S1050-1738(01)00149-9
  7. Wang L, Zhang Z, Wang Y, Zhang R, Chopp M. Treatment of stroke with erythropoietin enhances neurogenesis and angiogenesis and improves neurological function in rats. Stroke. 2004 ; 35 : 1732-7. https://doi.org/10.1161/01.STR.0000132196.49028.a4
  8. Zhang H, Vutskits L, Pepper MS, Kiss JZ. VEGF is a chemoattractant for FGF-2-stimulated neural progenitors. J Cell Biol. 2003 ; 163 : 1375-84. https://doi.org/10.1083/jcb.200308040
  9. Cho ZH, Chung SC, Jones JP et al. New fndings of the correlation between acupoints and corresponding brain cortices using functional MRI. Proc Natl Acad Sci. 1998 ; 95 : 2670-3. https://doi.org/10.1073/pnas.95.5.2670
  10. Kim JH, Min BI, Schmidt D, Lee HJ, Park DS. The difference between electroacupuncture only and electroacupuncture with manipulation on analgesia in rats. Neurosci Lett. 2000 ; 279 : 149-52. https://doi.org/10.1016/S0304-3940(99)00994-5
  11. Lee JH, Beitz AJ. The distribution of brainstem and spinal cord nuclei associated with different frequencies of electroacupuncture analgesia. Pain. 1993 ; 52 : 11-28. https://doi.org/10.1016/0304-3959(93)90109-3
  12. Shi R, Ji G, Zhao L, Wang S. Effects of electroacupuncture and twirling reinforcingreducing manipulations on volume of microcirculatory blood flow in cerebral pia mater. J Tradit Chin Med. 1998 ; 18 : 220-4.
  13. Wu MT, Hsieh JC, Xiong J et al. Central nervous pathway for acupuncture stimulation: localization of processing with functional MR imaging of the brain-preliminary experience. Radiology. 1999 ; 212 : 133-41. https://doi.org/10.1148/radiology.212.1.r99jl04133
  14. Park HJ, Lim S, Joo WS et al. Acupuncture prevents 6-hydroxydopamine-induced neuronal death in the nigrostriatal dopaminergic system in the rat Parkinson's disease model. Exp Neurol. 2003 ; 180 : 93-98. https://doi.org/10.1016/S0014-4886(02)00031-6
  15. Ha JY, Lee SH, Yin CS et al. The effect of manual acupuncture therapy on symptoms of the patients with idiopathic Parkinson's disease. J Korean Orient Med. 2003 ; 24 : 172-83.
  16. Kang JM, Park HJ, Choi YG et al. Acupuncture inhibits microglial activation and inflammatory events in the MPTP-induced mouse model. Brain Res. 2007 ; 1131 : 211-9. https://doi.org/10.1016/j.brainres.2006.10.089
  17. Chung JH, Lee EY, Jang MH et al. Acupuncture decreases ischemia-induced apoptosis and cell proliferation in dentate gyrus of gerbils. Neurological research. 2007 ; 29Suppl1 : S23-27. https://doi.org/10.1179/016164107X172239
  18. Kim EH, Kim YJ, Lee HJ et al. Acupuncture increases cell proliferation in dentate gyrus after transient global ischemia in gerbils. Neuroscience letters. 2001 ; 297 : 21-4. https://doi.org/10.1016/S0304-3940(00)01656-6
  19. Yang ZJ, Shen DH, Guo Xin, Sun FY. Electroacupuncture enhances striatal neurogenesis in adult rat brains after a transient cerebral middle artery occlusion. Acupuncture & electrotherapeutics research. 2005 ; 30 : 185-99.
  20. Ma JX, Yong Luo. Effects of electroacupuncture on expressions of angiogenesis factors and anti-angiogenesis factors in brain of experimental cerebral ischemic rats after reperfusion. Journal of tranditional Chinese medicine. 2008 ; 28(3) : 217-22. https://doi.org/10.1016/S0254-6272(08)60050-3
  21. Ou YW, Han L, Da CD et al. Influence of acupuncture upon expressing levels of basic fibroblast growth factor in rat brain following focal cerebral ischemia-evaluated by timeresolved fluorescence immunoassay. Neurological Research. 2001 ; 23(1) : 47-50. https://doi.org/10.1179/016164101101198271
  22. Wei G, Huang Y, Wu G et al. Regulation of glial cell line-derived neurotrophic factor expression by electroacupuncture after transient focal cerebral ischemia. Acupuncture & Electro-Therapeutics Research. 2000 ; 25(2) : 81-90.
  23. Wang SJ, Omori N, Li F et al. Functional improvement by electro-acupuncture after transient middle cerebral artery occlusion in rats. Neurological Research. 2003 ; 25(5) : 516-521. https://doi.org/10.1179/016164103101201751
  24. Sun FY, Guo X. Molecular and cellular mechanisms of neuroprotection by vascular endothelial growth factor. Journal of Neuroscience Research. 2003 ; 25(5) : 516-21.
  25. Yin CS, Jeong HS, Park HJ et al. A proposed transpositional acupoint system in a mouse and rat model. Research in Veterinary Science. 2008 ; 84 : 159-65. https://doi.org/10.1016/j.rvsc.2007.04.004
  26. Zhang X, Yuan Y, Kuang P, Wu W, Zhang F, Liu J. Effects of electro-acupuncture on somatostatin and pancreatic polypeptide in ischemic cerebrovascular diseases. J Tradit Chin Med. 1999 ; 19 : 54-8.
  27. Yoshimura S, Takagi Y, Harada J et al. Fgf-2 regulation of neurogenesis in adult hippocampus after brain injury. Proc Natl Acad Sci. 2001 ; 98 : 5874-9. https://doi.org/10.1073/pnas.101034998
  28. Teramoto T, Qiu J, Plumier JC, Moskowitz MA. EGF amplifies the replacement of parvalbuminexpressing striatal interneurons after ischemia. J Clin Invest. 2003 ; 111 : 1125-32. https://doi.org/10.1172/JCI200317170
  29. Koizumi H, Higginbotham H, Poon T, Tanaka T, Brinkman BC, Gleeson JG. Doublecortin maintains bipolar shape and nuclear translocation during migration in the adult forebrain. Nat Neurosci. 2006 ; 9 : 779-86. https://doi.org/10.1038/nn1704
  30. Emsley JG, Mitchell BD, Kempermann G, Macklis JD. Adult neurogenesis and repair of the adult CNS with neural progenitors, precursors, and stem cells. Prog Neurobiol. 2005 ; 75 : 321-41. https://doi.org/10.1016/j.pneurobio.2005.04.002
  31. Tohru K, Henrik A, P　r T, Rina K, Zaal K, Olle L. Intracerebral Infusion of Glial Cell Line-Derived Neurotrophic Factor Promotes Striatal Neurogenesis After Stroke in Adult Rats. Stroke. 2006 ; 37 : 2361-7. https://doi.org/10.1161/01.STR.0000236025.44089.e1
  32. Leung DW, Cachianes G, Kuang WJ, Goeddel DV, Ferrara N. Vascular endothelial growth factor is a secreted angiogenic mitogen. Science. 1989 ; 246 : 1306-9. https://doi.org/10.1126/science.2479986
  33. Lee JT, Yim YK. A literature study on the application of Sa-am acupuncture for the treatment of the stroke. The journal of Korean acupuncture & moxibustion society. 2006 ; 23(5) : 115-25.
  34. He YZ, Han B, Zheng SF et al. Effect of different acupuncture needle-retaining time on hemorheology in ischemic stroke patients. Zhen Ci Yan Jiu. 2007 ; 32(5) : 338-41.
  35. Li G, Jack CR Jr, Yang ES. An fMRI study of somatosensory-implicated acupuncture points in stable somatosensory stroke patients. J Magn Reson Imaging. 2006 ; 24(5) : 1018-24. https://doi.org/10.1002/jmri.20702
  36. Yang YJ, Kim YS, Shin MS et al. Effects of acupuncture on the intrastriatal hemorrhageinduced caspase3 expression and newly cell birth in rats. Neurol Res. 2007 ; 29 Suppl 1 : S65-71. https://doi.org/10.1179/016164107X172266
  37. Lu XCM, Williams AJ, Yao C et al. Microarray analysis of acute and delayed gene expression profile in rats after focal ischemic brain injury and reperfusion. J Neurosci Res. 2004 ; 77 : 843-57. https://doi.org/10.1002/jnr.20218
  38. Arezou SS, Hui S, Yun W, Barry JH, Cristina MB. Gene Expression Patterns in Mouse Cortical Penumbra After Focal Ischemic Brain Injury and Reperfusion. Journal of Neuroscience Research. 2008 ; 86 : 2912-24. https://doi.org/10.1002/jnr.21734
  39. Zhang RL, Zhang ZG, Chopp M. Ischemic stroke and neurogeneiss in the subventricular zone. Neuropharmacology. 2008 ; 55 : 345-52. https://doi.org/10.1016/j.neuropharm.2008.05.027
  40. Gros CG. Neurogenesis in the adult brain: death of a dogma. Nature Reviews Neuroscience. 2000 ; 1(1) : 67-73. https://doi.org/10.1038/35036235
  41. Zhang RL, Zhang ZG, Zhang L, Chopp M. Proliferation and differentiation of progenitor cells in the cortex and the subventricular zone in the adult rat after focal cerebral ischemia. Neuroscience. 2001 ; 105 : 33-41. https://doi.org/10.1016/S0306-4522(01)00117-8
  42. Brown JP, Couillard-Despres S, Cooper-Kuhn CM, Winkler J, Aigner L, Kuhn HG. Transient expression of doublecortin during adult neurogenesis. J Comp Neurol. 2003 ; 467 : 1-10. https://doi.org/10.1002/cne.10874
  43. Parent JM, Vexler ZS, Gong C, Derugin N, Ferriero DM. Rat forebrain neurogenesis and striatal neuron replacement after focal stroke. Ann Neurol. 2002 ; 52 : 802-13. https://doi.org/10.1002/ana.10393
  44. Ohab JJ, Carmichael ST. Poststroke neurogenesis: Emerging principles of migration and localization of immature neurons. The neuroscientist. 2008 ; 14(4) : 369-80. https://doi.org/10.1177/1073858407309545
  45. Alvarez-Buylla A, Lim DA. For the long run: maintaining germinal niches in the adult brain. Neuron. 2004 ; 41 : 683-6. https://doi.org/10.1016/S0896-6273(04)00111-4
  46. Keck PJ, Hauser SD, Krivi G et al. Vascular permeability factor, an endothelial cell mitogen related to PDGF. Science. 1989 ; 246 : 1309-12. https://doi.org/10.1126/science.2479987
  47. Sondell M, Lundborg G, Kanje M. Vascular endothelial growth factor has neurotrophic activity and stimulates axonal outgrowth, enhancing cell survival and Schwann cell proliferation in the peripheral nervous system. J Neurosci. 1999 ; 19 : 5731-40.
  48. Yang ZJ, Bao WL, Qiu MH et al. Role of vascular endothelial growth factor in neuronal DNA damage and repair in rat brain following a transient cerebral ischemia. J Neurosci Res. 2002 ; 70 : 140-9. https://doi.org/10.1002/jnr.10380