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Phenolic Contents and Antioxidant Activities of Six Edible Seaweeds

식용해조류 6종의 페놀성화합물 함량 및 항산화 활성

  • Kim, So Jung (Kyeongbuk Institute for Marine Bio-Industry) ;
  • Lee, Gunsup (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology) ;
  • Moh, Sang Hyun (Anti-aging Research Institute of BIO-FD&C Co., Ltd.) ;
  • Park, Jongbum (Department of Biological Science, Silla University) ;
  • Auh, Chung-Kyoon (Department of Biological Science, Mokpo National University) ;
  • Chung, Youngjae (Department of Life Science and Biotechnology, Shin Gyeong University) ;
  • Ryu, Tae Kwon (Risk Assessment Division, National Institute of Environmental Research) ;
  • Lee, Taek-Kyun (South Sea Environment Research Department, Korea Institute of Ocean Science & Technology)
  • 김소정 (경북해양바이오산업연구원) ;
  • 이건섭 (한국해양과학기술원 남해특성연구부) ;
  • 모상현 (바이오에프디엔씨 항노화연구소) ;
  • 박종범 (신라대학교 생명과학과) ;
  • 오정균 (국립목포대학교 생명과학과) ;
  • 정영재 (신경대학교 생명공학과) ;
  • 류태권 (국립환경과학원 위해성평가연구과) ;
  • 이택견 (한국해양과학기술원 남해특성연구부)
  • Received : 2013.05.13
  • Accepted : 2013.06.07
  • Published : 2013.06.30

Abstract

Phenolic contents and antioxidant activities were determined in the water and ethanol extracts from six species of edible seaweeds, Phaeophyceae (Laminaria japonica, Hizikia fusiformis and Undaria pinnatifida), Rhodophyceae (Porphyra tenera and Gracilaria verrucosa) and Chlorophyceae (Ulva lactuca). The highest extraction yield was observed in water extract (pH 8.0) of G. verrucosa (44.23 %) and phenolic content was the highest in ethanolic (75 %) extract of H. fusiformis (52.82 ${\mu}g/mg$). 25 % ethanolic extract from U. lactuca was found to have the highest DPPH radicals scavenging activity(19.29 %) and superoxide anion scavenging activitiy was higher in water extract (pH 8.0) from H. fusiformis (81.20 %) than that other seaweeds. Phenolic contents were strongly correlated with antioxidant activity in the six edible seaweeds extracts ($R^2$=0.852). Taken together, these results indicate that H fusiformis may be a excellent source for development of natural antioxidants.

갈조류(미역, 톳, 다시마), 홍조류(김, 꼬시래기) 및 녹조류(파래) 등 식용해조류 6종으로부터 제조한 추출물의 페놀성 화합물 함량과 항산화 활성을 분석하였다. 추출수율은 꼬시래기 분말을 물(pH 8.0)을 용매로 추출하였을 때 가장 높았으며(44.23 %), 페놀성 화합물은 톳을 75 % 에탄올 용액으로 추출하였을 때 가장 높았다(52.82 ${\mu}g/mg$). 25% 에탄올 용액으로 추출한 파래 추출물에서 가장 높은 DPPH 라디칼 소거활성이 나타났으며(19.29 %), superoxide 음이온 소거활성은 다른 해조류 추출물에 비해 물(pH 8.0)로 추출한 톳 추출물(81.20 %)에서 더 높았다. 6종의 식용해조류 추출물에서의 페놀성 화합물의 함량은 항산화 활성과 강하게 상관관계를 보였다(R2=0.852). 종합해 보면, 이러한 결과는 갈조류인 톳이 천연 항산화제 개발을 위한 좋은 원료가 될 수 있음을 의미한다.

References

  1. M. Kumar V. Gupta, P. Kumari, C. R. K. Reddy, B. Jha. "Assessment of nutrient composition and antioxidant potential of Caulerpaceae seaweeds". J Food Comp Anal vol. 24, pp. 270-278, 2011. DOI: http://dx.doi.org/10.1016/j.jfca.2010.07.007 https://doi.org/10.1016/j.jfca.2010.07.007
  2. G. K. Devi, K. Manivannan, G. Thirumaran, F. A. A. Rajathi, P. Anantharaman. "In vitro antioxidant activities of selected seaweeds from Southeast coast of India". Asian Pac. J. Trop. Med. vol. 4, no. 3, pp. 205-211, 2011. DOI: http://dx.doi.org/10.1016/S1995-7645(11)60070-9 https://doi.org/10.1016/S1995-7645(11)60070-9
  3. S. Meenakshi, S. Umayaparvathi, M. Arumugam, T. Balasubramanian. "In vitro antioxidant properties and FTIR analysis of two seaweeds of Gulf of Mannar". Asian Pac. J. Trop. Med. S66-S70, 2012. DOI: http://dx/doi.org/10.1016/S2221-1691(11)60126-3
  4. N. Bhaskar, K. Miyashita. "Lipid composition of Padina tetratomatica (Dictyotales, Phaeophyta), brown seaweed of the west coast of India". Ind J Fish vol. 52, pp. 263-268, 2005. DOI: http://dx.doi.org/10.5897/AJB11.2765
  5. S. K. Chandini, P. Ganesan, P. V. Suresh, N. Bhaskar. "Seaweeds as a source of nutritionally beneficial compounds-a review". J Food Sci Technol vol. 45, no. 1, pp. 1-13, 2008.
  6. T. Fujimura, K. Tsukahara, S. Moriwaki, T. Kitahara, T. Sano, Y. Takema. "Treatment of human skin with an extract of Fucus vesiculosus changes its thickness and mechanical properties." J. Cosmet. Sci. vol. 53, pp. 1-9, 2002.
  7. S. Kim, S. Woo, H. Yun, S. Yum, E. Choi, J.-R. Do, J.-H. Jo, D. Kim, S. Lee, T.-K. Lee. "Total Phenolic Contents and Biological Activities of Korean Seaweed Extracts". Food Sci. Biotechnol. vol. 14, no. 6, pp. 798-802, 2005.
  8. K. H. Wong, P. C. K. Cheung. "Nutritional evaluation of some subtropical red and green seaweeds. Part I-proximate composition, amino acid profiles and some physico-chemical properties". Food Chemistry, vol. 71, pp. 475-482, 2000. DOI: http://dx.doi.org/10.1016/S0308-8146(00)00175-8 https://doi.org/10.1016/S0308-8146(00)00175-8
  9. Y. V. Yuan, N. A. Walsh. "Antioxidant and antiproliferative activities of extracts from a variety of edible seaweeds". Food Chem. Toxicol., vol. 44, pp. 1144-1150, 2006. DOI: http://dx.doi.org/10.1016/j.fct.2006.02.002 https://doi.org/10.1016/j.fct.2006.02.002
  10. J. E. F. Cassolato, M. D. Noseda, C. A. Pujol, F. M. Pellizzari, E. B. Damonte, M. E. R. Duarte. "Chemical structure and antiviral activity of the sulfated heterorhamnan isolated from the green seaweed Gayralia oxysperma". Carbohydrate Research vol. 343, pp. 3085-3095, 2008. DOI: http://dx.doi.org/10.1016/j.carres.2008.09.014 https://doi.org/10.1016/j.carres.2008.09.014
  11. A. M. S. Mayer, A. D. Rodriguez, R. Berlinck, M. T. Hamann. "Marine pharmacology in 2005-6: marine compounds with antibacterial, anticoagulant, antifungal, anthelmitic, anti-inflammatory, antiprotozoal, and antiviral activities; affecting the cardiovascular, endocrine, immune and nervous systems and other miscellaneous mechanisms of action". Biochimica et Biophysica Acta, General Subjects vol. 1790, no. 5, pp. 283-308, 2009. DOI: http://dx.doi.org/10.1016/j.bbagen.2009.03.011 https://doi.org/10.1016/j.bbagen.2009.03.011
  12. C. Capannesi, I. Palchetti, M. Mascini, A. Parenti. "Electrochemical sensor and biosensor for polyphenols detection in olive oils". Food Chem. vol. 71, pp. 553-562, 2000. DOI: http://dx.doi.org/10.1016/S0308-8146(00)00211-9 https://doi.org/10.1016/S0308-8146(00)00211-9
  13. Y. Lu, L. Y. Foo. "Antioxidant and radical scavenging activities of polyphenols apple pomace". Food Chem. vol. 68, pp. 81-85, 2000. DOI: http://dx.doi.org/10.1016/S0308-8146(99)00167-3 https://doi.org/10.1016/S0308-8146(99)00167-3
  14. M. Oktay, I. Gulcin, I. Kufrevioglu. "Determination of in vitro antioxidant activity of fennel (Foeniculum vulgare) seed extracts." Lebensm.-Wiss U.-Technol. vol. 36, pp. 263-271, 2003. DOI: http://dx.doi.org/10.1016/S0023-6438(02)00226-8 https://doi.org/10.1016/S0023-6438(02)00226-8
  15. S. Latha, M. Daniel. "Phenolic antioxidants of some common pulses". J Food Sci Technol vol. 38, pp. 272-273, 2001.
  16. G. Ruberto, M. T. Baratta, D. M. Biondi, V. Amico. "Antioxidant activity of extracts of the marine algal genus Cystoseira in a micellar model system". J Appl Phycol vol. 13, pp. 403-407, 2001. DOI: http://dx.doi.org/10.1023/A:1011972230477 https://doi.org/10.1023/A:1011972230477
  17. T. Kuda, T. Ikemori. "Minerals, polysaccharides and antioxidant properties of aqueous solutions obtained from macroalgal beachcasts in the Noto Peninsula, Ishikawa, Japan". Food Chem vol. 112, pp. 575-581, 2009. DOI: http://dx.doi.org/10.1016/j.foodchem.2008.06.008 https://doi.org/10.1016/j.foodchem.2008.06.008
  18. L. Cavas, K. Yurdakoc. "An investigation on the antioxidant status of the invasive alga Caulerpa racemosa var. cylindracea (Sonder) Verlaque, Huisman, et Boudoresque (Caulerpales, Chlorophyta)". J. Exp. Mar. Biol. Ecol. vol. 325, pp. 189-200, 2005. DOI: http://dx.doi.org/10.1016/j.jembe.2005.05.002 https://doi.org/10.1016/j.jembe.2005.05.002
  19. H. L. Huang, B. G. Wang. "Antioxidant capacity and liphophilic contents of seaweeds collected from the Qingdao coastline". J Agric. Food Chem. vol. 52, pp. 4993-4997, 2004. DOI: http://dx.doi.org/10.1021/jf049575w https://doi.org/10.1021/jf049575w
  20. W-Ch, Fang, C. H. Kao. "Enhanced peroxidase activity in rice leaves in response to excess iron, copper and zinc". Plant Sci. vol. 158, pp. 71-76, 2000. DOI: http://dx.doi.org/10.1016/S0168-9452(00)00307-1 https://doi.org/10.1016/S0168-9452(00)00307-1
  21. R. K. Tewari, P. Kumar, P. N. Sharma, S. S. Bisht. "Modulation of oxidative stress responsive enzymes by excess cobalt". Plant Sci. vol. 162, pp. 381-88, 2002. DOI: http://dx.doi.org/10.1016/S0168-9452(01)00578-7 https://doi.org/10.1016/S0168-9452(01)00578-7
  22. Y. L. Chew, Y. Y. Lim,, M. Omar, K. S. Khoo. "Antioxidant activity of three edible seaweeds from two areas in South East Asia". LWT vol. 41, pp. 1067-1072, 2008. DOI: http://dx.doi.org/10.1016/j.lwt.2007.06.013 https://doi.org/10.1016/j.lwt.2007.06.013
  23. H. Wiseman, B. Halliwell. "Damage to DNA by reactive oxygen and nitrogen species: Role of inflammatory disease and progression to cancer". Biochem. J. vol. 313, pp. 17-29, 1996. https://doi.org/10.1042/bj3130017
  24. R. A. Larson. "Plant defenses against oxidative stress". Arch. Insect Biochem. Physiol. vol. 29, pp. 175-186, 1995. DOI: http://dx.doi.org/10.1002/arch.940290207 https://doi.org/10.1002/arch.940290207
  25. N. Singh, P. S. Rajini. "Free radical scavenging activity of an aqueous extract of potato peel". Food Chemistry, vol. 85, pp. 611-616, 2004. DOI: http://dx.doi.org/10.1016/j.foodchem.2003.07.003 https://doi.org/10.1016/j.foodchem.2003.07.003
  26. T. Kuda, M. Tsunekawa, H. Goto, Y. Araki. "Antioxidant properties of four edible algae harvested in the Noto Peninsula, Japan". J Food Composit Anal vol. 18, pp. 625-633, 2005. DOI: http://dx.doi.org/10.1016/j.jfca.2004.06.015 https://doi.org/10.1016/j.jfca.2004.06.015
  27. K. Ganesan, S. K. Kumar, P. V. Subba Rao. "Comparative assessment of antioxidant activity in three edible species of green seaweed, Enteromorpha from Okha, Northwest coast of India". Innov Food Sci Emerg Technol vol. 12, pp. 73-78, 2011. DOI: http://dx.doi.org/10.1016/j.ifset.2010.11.005 https://doi.org/10.1016/j.ifset.2010.11.005
  28. M. Cho, H-S. Lee, I-J. Kang, M-H Won, S You. "Antioxidant properties of extract and fractions from Enteromorpha prolifera, a type of green seaweed". Food Chem vol. 127, pp. 999-1006, 2011. DOI: http://dx.doi.org/10.1016/j.foodchem.2011.01.072 https://doi.org/10.1016/j.foodchem.2011.01.072
  29. P. Prieto, M. Pineda, M. Aguilar. "Spectrophotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex: Specific application to the determination of vitamin E". Analytical Biochemistry vol. 269, pp. 337-341, 1999. DOI: http://dx.doi.org/10.1006/abio.1999.4019 https://doi.org/10.1006/abio.1999.4019

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