Asian Pacific Journal of Cancer Prevention

  • 제14권4호
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  • Pages.2289-2293
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  • 2013
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  • 1513-7368(pISSN)
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  • 2476-762X(eISSN)
아시아태평양암예방학회 (Asian Pacific Journal of Cancer Prevention)
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Aberrant Expression of miR-20a and miR-203 in Cervical Cancer

  • Zhao, Shan (Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University) ;
  • Yao, De-Sheng (Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University) ;
  • Chen, Jun-Ying (Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University) ;
  • Ding, Nan (Department of Gynecologic Oncology, the Affiliated Tumor Hospital of Guangxi Medical University)
  • 발행 : 2013.04.30
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초록

MicroRNAs (miRNAs) are small, non-coding RNAs that are critical regulators of various diseases. MicroRNA-20a (miR-20a) and microRNA-203 (miR-203) have previously shown significant alteration in a range of cancers. In this study, the expression levels of miR-20a and miR-203 in 100 cervical cancer tissues were detected by qRT-PCR and compared to patient matched-nontumor cervical tissues. Correlations between expression level and clinicopathologic characteristics of cervical cancer were also analyzed. Finally, we studied the effect of miR-20a and miR-203 on cell proliferation in cervical cancer cell lines by MTT. We found that the expression level of miR-20a (P<0.001) was significantly higher in cervical cancer patients than in healthy controls, while that of miR-203 (P<0.001) was lower. Aberrant expression of miR-20a was correlated with lymph node metastasis (LNM), histological grade and tumor diameter, but down-regulated miR-203 was correlated with LNM only. Furthermore, we found that over-expression of miR-203 decreased cell proliferation, while reduction of miR-20a also prevented tumor progression. Our results support the involvement of miR-20a and miR-203 in cervical tumorigenesis. We propose that miRNAs might be used as therapeutic agents for cervical cancer.

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참고문헌

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