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Inhibitory Effects of Tualang Honey on Experimental Breast Cancer in Rats: A Preliminary Study

  • Kadir, Erazuliana Abd (Pharmacology Department, School of Medical Sciences, Universiti Sains Malaysia) ;
  • Sulaiman, Siti Amrah (Pharmacology Department, School of Medical Sciences, Universiti Sains Malaysia) ;
  • Yahya, Nurul Khaiza (Immunology Department, School of Medical Sciences, Universiti Sains Malaysia) ;
  • Othman, Nor Hayati (Pathology Department, School of Medical Sciences, Universiti Sains Malaysia)
  • Published : 2013.04.30

Abstract

The study was conducted to determine the effect of Malaysian jungle Tualang Honey (TH) on development of breast cancer induced by the carcinogen 7,12-dimethylbenz(${\alpha}$)anthracene (DMBA) in rats. Forty nulliparous female Sprague-Dawley rats were given 80 mg/kg DMBA then randomly divided into four groups: Group 1 served as a Control while Groups 2, 3 and 4 received 0.2, 1.0 or 2.0 g/kg bodyweight/day of TH, respectively, for 150 days. Results showed that breast cancers in the TH-treated groups had slower size increment and smaller mean tumor size (${\leq}2cm^3$) compared to Controls (${\leq}8cm^3$). The number of cancers developing in TH-treated groups was also significantly fewer (P<0.05). Histological grading showed majority of TH-treated group cancers to be of grade 1 and 2 compared to grade 3 in controls. There was an increasing trend of apoptotic index (AI) seen in TH-treated groups with increasing dosage of Tualang Honey, however, the mean AI values of all TH-treated groups were not significantly different from the Control value (p>0.05). In conclusion, Tualang Honey exerted positive modulation effects on DMBA-induced breast cancers in rats in this preliminary study.

Acknowledgement

Supported by : FAMA

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