Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
권호 표지
DOI QR코드

DOI QR Code

Interferon Stimulated Gene - ISG15 is a Potential Diagnostic Biomarker in Oral Squamous Cell Carcinomas

  • Published : 2013.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

Background: Cancer diagnostic biomarkers have a wide range of applications that include early detection of oral precancerous lesions and oral squamous cell carcinomas, and assessing the metastatic status of lesions. The interferon stimulated ISG15 gene encodes an ubiquitin-like protein, which conjugates to stabilize activation status of associated proteins. Hence a deregulated expression of ISG15 may promote carcinogenesis. Indeed overexpression of ISG15 has been observed in several cancers and hence it has been proposed as a strong candidate cancer diagnostic biomarker. Given the emerging relationship between malignant transformation and ISG15, we sought to examine the expression pattern of this gene in tumor biopsies of oral squamous cell carcinoma (OSCC) tissues collected from Indian patients. Materials and Methods: Total RNA isolated from thirty oral squamous cell carcinoma tissue biopsy samples were subjected to semi-quantitative RT-PCR with ISG15 specific primers to elucidate the expression level. Results: Of the thirty oral squamous cell carcinomas that were analyzed, ISG15 expression was found in twenty four samples (80%). Twelve samples expressed low level of ISG15, six of them expressed moderately, while the rest of them expressed very high level of ISG15. Conclusions: To the best of our knowledge, the results show for the first time an overexpression of ISG15 in up to 80% of oral squamous cell carcinoma tissues collected from Indian patients. Hence ISG15 may be explored for the possibility of use as a high confidence diagnostic biomarker in oral cancers.

Keywords

References

  1. Andersen JB, Hassel BA (2006a). The interferon regulated ubiquitin-like protein, ISG15, in tumorigenesis: Friend or foe. Cytokine and Growth Factor Reviews, 17, 411-21. https://doi.org/10.1016/j.cytogfr.2006.10.001
  2. Andersen JB, Aaboe M, Borden EC, et al (2006b). Stage-associated overexpression of the ubiquitin-like protein, ISG15, in bladder cancer. Br J Cancer, 94, 1465-71. https://doi.org/10.1038/sj.bjc.6603099
  3. Arellano-Garcia ME, Li R, Liu X, et al (2010). Identification of tetranectin as a potential biomarker for metastatic oral cancer. Int J Mol Sc, 11, 3106-21. https://doi.org/10.3390/ijms11093106
  4. Bektas N, Noetzel E, Veeck J, et al (2008). The ubiquitin-like molecule interferon-stimulated gene 15 (ISG15) is a potential prognostic marker in human breast cancer. Breast Cancer Res, 10, 58.
  5. BlomstromDC, Fahey D, Kutny R, Korant BD, Knight Jr E (1986). Molecular characterization of the interferon-induced 15-kDa protein. Molecular cloning and nucleotide and amino acid sequence. J Biol Chem, 261, 8811-16.
  6. Boddy MN, Howe K, Etkin LD, Solomon E, Freemont PS (1996). PIC 1a novel ubiquitin-like protein which interacts with the PML component of a multiprotein complex that is disrupted in acute promyelocytic leukaemia. Oncogene, 13, 971-82.
  7. D'Cunha J, Knight E Jr, Haas AL, Truitt RL, Borden EC (1996). Immunoregulatory properties of ISG15, an interferon-induced cytokine. Proc Natl Acad Sci, 93, 211-15. https://doi.org/10.1073/pnas.93.1.211
  8. Desai SD, Haas AL, Wood LM, et al (2006). Elevated expression of isg15 in tumor cells interferes with the ubiquitin/26s proteasome pathway. Cancer Res, 66, 921-28. https://doi.org/10.1158/0008-5472.CAN-05-1123
  9. Durfee LA, Lyon N, Seo K, Huibregtse JM (2010). The ISG15 conjugation system broadly targets newly synthesized proteins: implications for the antiviral function of ISG15. Mol Cell, 38, 722-32. https://doi.org/10.1016/j.molcel.2010.05.002
  10. Ginos MA, Page GP, Michalowicz BS, et al (2004). Identification of a gene expression signature associated with recurrent disease in squamous cell carcinoma of the head and neck. Cancer Res, 64, 55-63. https://doi.org/10.1158/0008-5472.CAN-03-2144
  11. Herrmann J, Lerman LO, Lerman A (2007). Ubiquitin and ubiquitin-like proteins in protein regulation. Circ Res,100, 1276-91. https://doi.org/10.1161/01.RES.0000264500.11888.f0
  12. He-Xin S, Kai Y, Xing L, Xin-Yi L, et al (2010). Positive regulation of interferon regulatory factor 3 activation by herc5 via ISG15 modificatio. MolCell Biol,30, 2424-36.
  13. Hoek K, Rimm DL,Williams K, et al (2004). Expression profiling reveals novel pathways in the transformation of melanocytes to melanomas. Cancer Res, 64, 5270-8. https://doi.org/10.1158/0008-5472.CAN-04-0731
  14. Iacobuzio-Donahue CA, Maitra A, Olsen M, et al (2003). Exploration of global gene expression patterns in pancreatic adenocarcinoma using cDNA microarrays. Am J Pathol, 162, 1151-62. https://doi.org/10.1016/S0002-9440(10)63911-9
  15. Indian Genome Variation Consortium (2008). Genetic landscape of the people of India: a canvas for disease gene exploration. J Genet, 87, 3-20. https://doi.org/10.1007/s12041-008-0002-x
  16. Kamitani T, Kito K, Nguyen HP, et al (1997). Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein. J Biol Chem, 272, 28557-62. https://doi.org/10.1074/jbc.272.45.28557
  17. Lang-Ming C, Chien-Wei L, Kai-Ping C, et al (2009). Enhanced interferon signaling pathway in oral cancer revealed by quantitative proteome analysis of microdissected specimens using 16o/18o labeling and integrated two-dimensional LC-ESI-MALDI Tandem MS. Mol Cell Proteomics. 8, 1453-74. https://doi.org/10.1074/mcp.M800460-MCP200
  18. Laurence A, Pesu M, Silvennoinen O, O'Shea J (2012). JAK kinases in health and disease: an update. Open Rheumatol J, 6, 232-44. https://doi.org/10.2174/1874312901206010232
  19. Liu YC, Pan J, Zhang C, et al (1999). A MHC-encoded ubiquitin-like protein (FAT10) binds noncovalently to the spindle assembly checkpoint protein MAD2. Proc Natl Acad Sci USA, 96, 4313-18. https://doi.org/10.1073/pnas.96.8.4313
  20. Malakhova O, Malakhov M, Hetherington C, Zhang DE (2002). UBP43 (USP18) specifically removes ISG15 from conjugated proteins. J Biol Chem. 277, 14703-11. https://doi.org/10.1074/jbc.M111527200
  21. Mangone FRR, Walder F, Maistro S et al (2010). Smad2 and Smad6 as predictors of overall survival in oral squamous cell carcinoma patients. Mol Cancer, 9, 106. https://doi.org/10.1186/1476-4598-9-106
  22. O'Donnell RK, Kupferman M, Wei SJ, et al (2005). Gene expression signature predicts lymphatic metastasis in squamous cell carcinoma of the oral cavity. Oncogene, 24, 1244-51. https://doi.org/10.1038/sj.onc.1208285
  23. Padovan E, Terracciano L, Certa U, et al (2002). Interferon stimulated gene 15 constitutively produced by melanoma cells induces e-cadherin expression on human dendritic cells.Cancer Res, 62, 3453-58.
  24. Pitha-Rowe IF, Pitha PM (2007). Viral defense, carcinogenesis and ISG15: novel roles for an old ISG. Cytokine Growth Factor Rev, 18, 409-17. https://doi.org/10.1016/j.cytogfr.2007.06.017
  25. Pitha-Rowe I, Petty WJ, Feng Q, et al (2004). Microarray analyses uncover ube1l as a candidate target gene for lung cancer chemoprevention. Cancer Res, 64, 8109-15. https://doi.org/10.1158/0008-5472.CAN-03-3938
  26. Rajkumar T, Sabitha K, Vijayalakshmi N, et al (2011). Identification and validation of genes involved in cercival tumorigenesis. BMC Cancer, 11, 80. https://doi.org/10.1186/1471-2407-11-80
  27. Reis PP, Tomenson M, Cervigne NK, et al (2010). Programmed cell death 4 loss increases tumor cell invasion and is regulated by miR-21 in oral squamous cell carcinoma. Mol Cancer, 9, 238. https://doi.org/10.1186/1476-4598-9-238
  28. Roberts ZJ, Goutagny N, Perera PY, et al (2007). The chemotherapeutic agent DMXAA potently and specifically activates the TBK1-IRF-3 signaling axis. J Exp Med, 204, 1559-69. https://doi.org/10.1084/jem.20061845
  29. Saintigny P, El-Naggar AK, Papadimitrakopoulou V et al (2009). DeltaNp63 overexpression, alone and in combination with other biomarkers, predicts the development of oral cancer in patients with leukoplakia. Clin Cancer Res, 15, 6284-91. https://doi.org/10.1158/1078-0432.CCR-09-0498
  30. Saitoh T, Tun-Kyi A, Ryo A, et al (2006). Negative regulation of interferon-regulatory factor 3-dependent innate antiviral response by the prolyl isomerase Pin1. Nat Immunol, 7, 598-605. https://doi.org/10.1038/ni1347
  31. Satake H, Tamura K, Furihata M, et al (2010). The ubiquitin-like molecule interferon-stimulated gene 15 is overexpressed in human prostate cancer. Oncol Rep, 23, 11-16.
  32. Takeuchi T, Yokosawa H (2005). ISG15 modification of Ubc13 suppresses its ubiquitin-conjugating activity. Biochem Biophys Res Commun, 336, 9-13. https://doi.org/10.1016/j.bbrc.2005.08.034
  33. Tripathi SC, Matta A, Kaur J, et al (2010). Nuclear S100A7 is associated with poor prognosis in head and neck cancer. PLoS One, 5, 11939. https://doi.org/10.1371/journal.pone.0011939
  34. Yanai H, Negishi H, Taniguchi T (2012). The IRF family of transcription factors: inception, impact and implications in oncogenesis. Oncoimmunology, 8, 1376-86.
  35. Ye H, Yu T, Temam S, et al (2008). Transcriptomic dissection of tongue squamous cell carcinoma. BMC Genomics, 9, 69. https://doi.org/10.1186/1471-2164-9-69
  36. Zhao C, Denison C, Huibregtse JM, Gygi S, Krug RM (2005). Human ISG15 conjugation targets both IFN-induced and constitutively expressed proteins functioning in diverse cellular pathways. Proc Natl Acad Sci USA, 102, 10200-5. https://doi.org/10.1073/pnas.0504754102
  37. Zou W, Papov V, Malakhova O, et al (2005). ISG15 modification of ubiquitin E2 Ubc13 disrupts its ability to form thioester bond with ubiquitin. Biochem Biophys Res Commun, 336, 61-8. https://doi.org/10.1016/j.bbrc.2005.08.038

Cited by

  1. A Novel Heterozygous Mutation (F252Y) in Exon 7 of the IRF6 Gene is Associated with Oral Squamous Cell Carcinomas vol.14, pp.11, 2013, https://doi.org/10.7314/APJCP.2013.14.11.6803
  2. Interferon Apha 2b for Treating Patients with JAK2V617F Positive Polycythemia Vera and Essential Thrombocytosis vol.15, pp.4, 2014, https://doi.org/10.7314/APJCP.2014.15.4.1681
  3. Interferon Induced Transmembrane Protein-1 Gene Expression is a Biomarker for Early Detection of Invasive Potential of Oral Squamous Cell Carcinomas vol.17, pp.4, 2016, https://doi.org/10.7314/APJCP.2016.17.4.2297
  4. Interferon-stimulated gene 15 modulates cell migration by interacting with Rac1 and contributes to lymph node metastasis of oral squamous cell carcinoma cells pp.1476-5594, 2019, https://doi.org/10.1038/s41388-019-0731-8