Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Effect of Botulinum Toxin A on Proliferation and Apoptosis in the T47D Breast Cancer Cell Line

  • Bandala, Cindy (Department of Research Support, Instituto Nacional de Rehabilitacion) ;
  • Perez-Santos, Jose Luis Martin (Vice-rectory of Research and Postgraduate Studies, Benemerita Universidad Autonoma de Puebla) ;
  • Lara-Padilla, Eleazar (Molecular Oncology and Oxidative Stress Laboratory, Escuela Superior de Medicina, Instituto Politecnico Nacional) ;
  • Delgado Lopez, Ma. Guadalupe (Laboratory of Cellular Biology, Centro de Investigacion Biomedica de Oriente, Instituto Mexicano del Seguro Social) ;
  • Anaya-Ruiz, Maricruz (Laboratory of Cellular Biology, Centro de Investigacion Biomedica de Oriente, Instituto Mexicano del Seguro Social)
  • Published : 2013.02.28
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Abstract

The present study was performed to assess the activity of the botulinum toxin A on breast cancer cells. The T47D cell line was exposed to diverse concentrations of the botulinum toxin A and cell viability and apoptosis were estimated using MTT and propidium iodine/annexin V methods, respectively. Botulinum toxin A exerted greater cytotoxic activity in T47D cells in comparison with MCF10A normal cells; this appeared to be via apoptotic processes caspase-3 and -7. In conclusion, botulinum toxin A induces caspase-3 and -7 dependent apoptotic processes in the T47D breast cancer cell line.

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References

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