Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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MicroRNA-21 Regulates the Invasion and Metastasis in Cholangiocarcinoma and May Be a Potential Biomarker for Cancer Prognosis

  • Huang, Qiang (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University) ;
  • Liu, Lei (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University) ;
  • Liu, Chen-Hai (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University) ;
  • You, Hao (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University) ;
  • Shao, Feng (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University) ;
  • Xie, Fang (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University) ;
  • Lin, Xian-Sheng (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University) ;
  • Hu, San-Yuan (Department of General Surgery, Qilu Hospital of Shandong University) ;
  • Zhang, Chuan-Hai (Department of General Surgery, Affiliated Provincial Hospital of Anhui Medical University)
  • Published : 2013.02.28
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Abstract

Background: MicroRNAs are noncoding RNA molecules that posttranscriptionally regulate gene expression. The aim of this study was to determine the role of microRNA-21 in cholangiocarcinomas and its relationship to cholangiocarcinoma RBE cell capacity for invasion and metastasis. Methods: MicroRNA-21 expression was investigated in 41 cases of cholangiocarcinoma samples by in situ hybridization and real-time PCR. Influence on cholangiocarcinoma cell line invasion and metastasis was analyzed with microRNA-21 transfected cells. In addition, regulation of reversion-inducing-cysteine-rich protein with kazal motifs (RECK) by microRNA-21 was elucidated to identify mechanisms. Results: In situ hybridization and real-time quantitative PCR results for patients with lymph node metastasis or perineural invasion showed significantly high expression of microRNA-21 (P<0.05). There was a dramatic decrease in cholangiocarcinoma cell line invasion and metastasis ability after microRNA-21 knockdown (P<0.05). However, overexpression significantly increased invasion and metastasis (P<0.05). Real-time PCR and Western-blot analysis showed that microRNA-21 could potentially inhibit RECK expression in RBE cells. Survival analysis showed that patients with higher expression levels of microRNA-21 more often had a poor prognosis (P<0.05). Conclusions: MicroRNA-21 may play an important role in cholangiocarcinoma invasion and metastasis, suggesting that MicroRNA-21 should be further evaluated as a biomarker for predicting cholangiocarcinoma prognosis.

Keywords

References

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