Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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Nested Multiplex PCR Based Detection of Human Papillomavirus in Cervical Carcinoma Patients of North- East India

  • Das, Debabrata (DBT Centre for Molecular Biology and Cancer Research, Dr. B. Borooah Cancer Institute) ;
  • Rai, Avdhesh Kumar (DBT Centre for Molecular Biology and Cancer Research, Dr. B. Borooah Cancer Institute) ;
  • Kataki, Amal Chandra (Department of Gynecologic Oncology, Dr. B. Borooah Cancer Institute) ;
  • Barmon, Debabrata (Department of Gynecologic Oncology, Dr. B. Borooah Cancer Institute) ;
  • Deka, Pankaj (Department of Gynecologic Oncology, Dr. B. Borooah Cancer Institute) ;
  • Sharma, Jagannath Dev (Department of Pathology, Dr. B. Borooah Cancer Institute) ;
  • Sarma, Anupam (Department of Pathology, Dr. B. Borooah Cancer Institute) ;
  • Shrivastava, Sushruta (Department of Gynecologic Oncology, Dr. B. Borooah Cancer Institute) ;
  • Bhattacharyya, Mouchumee (Department of Radiation Oncology, Dr. B. Borooah Cancer Institute) ;
  • Kalita, Apurba Kumar (Department of Radiation Oncology, Dr. B. Borooah Cancer Institute) ;
  • Sharma, Mousumi (Department of Pathology, Dr. B. Borooah Cancer Institute) ;
  • Ahmed, Shiraj (Department of Pathology, Dr. B. Borooah Cancer Institute)
  • Published : 2013.02.28
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Abstract

Background: Persistent infection of one or more of about 15 high-risk human papillomaviruses (HR-HPVs), most commonly HPV types 16/18, has a significant role in cervical cancer initiation and progression. There are limited data available from north-east India about HPV prevalence though this region has high incidence rates of cervical cancer. The aim of this study was to investigate the HPV genotypes prevalent in cervical cancer patients of north-east India. Materials and Methods: We analyzed 107 cervical cancer patient samples. Nested multiplex PCR assays were employed for detection of 13 high risk and 5 low risk HPV types. Results: HPV was confirmed in 105 samples. The presence of 6 'carcinogenic' HPV types, HPV-16 (88%), -18 (15%), -31(4%),-45 (3%), -59 (4%), -58(1%), and one non carcinogenic, HPV-6/11 (6%), was recorded. Among various demographic and clinical factors only tumour stage showed a statistically significant association with HPV type infection (P=0.019). Conclusions: We suggest that the most prevalent genotype is HPV-16 followed by HPV-18 in cervical carcinoma patients of the north-eastern region of India. Advanced tumour stage may be associated with increased possibility of harbouring multiple HPV genotypes.

Keywords

References

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