Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Plant Regeneration from Anther Culture of Panax ginseng

  • Lee, Hee-Young (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Khorolragchaa, Altanzul (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Sun, Myung-Suk (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Kim, Young-Joon (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Kim, Yu-Jin (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Kwon, Woo-Seang (Department of Oriental Medicinal Materials and Processing, Kyung Hee University) ;
  • Yang, Deok-Chun (Department of Oriental Medicinal Materials and Processing, Kyung Hee University)
  • Received : 2013.06.05
  • Accepted : 2013.06.21
  • Published : 2013.06.30
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Abstract

The research concerned of the regeneration of plants from embryos obtained from anther cultures of ginseng (Panax ginseng C. A. Meyer). The aim was to determine the influence of the regeneration medium on the efficiency of the regeneration process. We conducted to determine the optimum conditions such as cold pretreatment, plant growth regulators and carbon sources on anther culture of P. ginseng. Highest callus formation rate was obtained when flower buds pretreated at $4^{\circ}C$ for 1 day. Among the treated growth regulators with various degrees of concentration in Murashige and Skoog's (MS) medium, 4.53 ${\mu}M$ of 2.4-dichlorophenoxyacetic acid and 4.44 ${\mu}M$ of 6-benzylaminopurine gives the most responsive callus with the frequency of 73.89% and 129.53 g of fresh weight. When we used 3-9% of sucrose and maltose among the different kinds and various concentrations of carbohydrates, callus was formed highest 67.29% in the medium with 3% of sucrose. Shoots induced from callus supplemented with 28.9 ${\mu}M$ of gibberellic acid and rooted in Gamborg's B5 medium supplemented with 14.7 ${\mu}M$ of indole-3-butyric acid.

Keywords

References

  1. Arya, S., J.R. Liu and T. Eriksson. 1991. Plant regeneration from protoplasts of Panax ginseng (C.A. Meyer) through somatic embryogenesis. Plant Cell Rep. 10:277-281.
  2. Batty, N. and J. Dunwell. 1989. Effect of maltose on response of potato anthers in culture. Plant Cell Tiss. Org. Cult. 18:221-226. https://doi.org/10.1007/BF00047748
  3. Chang, W.C. and Y.I. Hsing. 1980. Plant regeneration through somatic embryogenesis in root-derived callus of ginseng (Panax ginseng C. A. Meyer). Theor. Appl. Genet. 57: 133-135.
  4. Chen, L.J., X.Y. Zhu, L. Gu and J. Wu. 2005. Efficient callus induction and plant regeneration from anther of Chinese narcissus (Narcissus tazetta L. var. chinesis Roem). Plant Cell Rep. 24:401-407. https://doi.org/10.1007/s00299-005-0958-4
  5. Choi, Y.E., D.C. Yang, E.S. Yoon and K.T. Choi. 1998. Plant regeneration via adventitious bud formation from cotyledon explants of Panax ginseng C. A. Meyer. Plant Cell Rep. 17:731-736. https://doi.org/10.1007/s002990050474
  6. Choi, Y.E., D.C. Yang, H.S. Kim and K.T. Choi. 1997. Distribution and changes of reserve materials in cotyledon cells of Panax ginseng related to direct somatic embryogenesis and germination. Plant Cell Rep. 16:841-846. https://doi.org/10.1007/s002990050331
  7. Finnie, S.J., W. Powll and A.F. Dyer. 1989. The effect of carbohydrate composition and concentration on anther culture response in barley (Hordeum vulgare L.). Plant Breed. 103:110-118. https://doi.org/10.1111/j.1439-0523.1989.tb00358.x
  8. Gamborg, O.L., R.A. Miller and O. Ojima. 1968. Nutrient requirements of suspension cultures of soybean root cell. Exp. Cell Res. 50:151-158. https://doi.org/10.1016/0014-4827(68)90403-5
  9. Gorecka, K., D. Krzyzanowska, W. Kiszczak and U. Kowalska. 2009. Plant regeneration from carrot (Daucus carota L.) anther culture derived embryos. Acta Physiol. Plant. 31:1139-1145. https://doi.org/10.1007/s11738-009-0332-1
  10. Gosal, S.S., A.S. Sindhu, J.S. Sandhu, R. Sandhu-Gill, B. Singh, G.S. Khera, G.S. Sidhu and H.S. Dhaliwal. 1997. Haploidy in rice 1-35. In Jain, S.M., S.K. Sopory and R.E. Veilleux (eds.), In vitro Haploid Production in Higher Plants, Kluwer Academic Publishers, Dordrecht, The Netherlands.
  11. Hu, H. 1997. In vitro induced haploids in wheat. In Jain 73-97. S.M. In Jain S.M., S.K. Sopory and R.E. Veilleux (eds.), In vitro haploid production in higher plants, Kluwer Academic Publishers, Dordrecht, The Netherlands. pp. 73-97.
  12. Keller, W.A., T. Rajhathy and J. Lacapra. 1975. In vitro production of plants from pollen in Brassica campestris. Can. J. Genet. Cytol. 17:655-666. https://doi.org/10.1139/g75-081
  13. Kim, H.B., C.W. Kang, B.S. Kim, J.K. Kwon, I.J. Yu, Y.S. Roh, S.Y. Nah, S. Ejaz and J.H. Kim. 2010. Beneficial role of ginseng saponin on hemodynamic functions of porcine blood vessel. J. Ginseng Res. 34:314-320. https://doi.org/10.5142/jgr.2010.34.4.314
  14. Lee, J.H., Y.J. Kim, D.Y. Jung, J.S. Shim, I.H. Kim and D.C. Yang. 2009. In vitro induction of tetraploid roots by various pretreatments from anther of Panax ginseng C. A. Meyer. J. Ginseng Res. 33:65-71. https://doi.org/10.5142/JGR.2009.33.1.065
  15. Lim, H.T., H.S. Lee and T. Eriksson. 1997. Regeneration of Panax ginseng C.A.Meyer by organogenesis and nuclear DNA analysis of regenerants. Plant Cell Tiss. Org. Cult. 49:179-187. https://doi.org/10.1023/A:1005820210895
  16. Loh, C.S. and D.S. Ingram. 1982. Production of haploid plants from anther cultures and secondary embryods of winter oilseed rape, Brassica napus ssp. oleifera. New Phytol. 91:507-516. https://doi.org/10.1111/j.1469-8137.1982.tb03329.x
  17. Murashige T. and F.A. Skoog. 1962. Revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol. Plant. 15:473-497. https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  18. Navarro-Alvarez, W., P.S. Baenziger, K.M. Eskridge, M. Hugo and V.D. Gustafson. 1994. Addition of colchicine to wheat anther culture media to increase doubled haploid plant production. Plant Breed. 112:192-198. https://doi.org/10.1111/j.1439-0523.1994.tb00670.x
  19. Schenk, R.U. and A.C. Hildebrandt. 1972. Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures. Can. J. Bot. 50:199-204. https://doi.org/10.1139/b72-026
  20. Solvari, S. and O. Schieder. 1987. Influence of sucrose and melibiose on barley anther cultures in starch media. Plant Breed. 99:164-171. https://doi.org/10.1111/j.1439-0523.1987.tb01167.x
  21. Sugaya, A., M. Yuzurihara, T. Tsuda, K. Yasuda, K. Kajiwara and E. Sugaya. 1998. Proliferative effect of ginseng saponin on neurite extension of primary cultured neurons of the rat cerebral cortex. J. Ethnopharmacol. 22:173-181.
  22. Tang, W. 2000. High-frequency plant regeneration via somatic embryogenesis and organogenesis and in vitro flowering of regenerated plantlets in Panax ginseng. Plant Cell Rep. 19:727-732. https://doi.org/10.1007/s002999900170
  23. Trejo-Tapia, G., U.M. Amaya, G.S. Morales, A.D.J. Sanchez, B.M. Bonfil, M. Rodriguez-Monroy and A. Jimenez-Aparicio. 2002. The effects of cold-pretreatment, auxin and carbon source on anther culture of rice. Plant Cell Tiss. Org. Cult. 71:41-46. https://doi.org/10.1023/A:1016558025840
  24. Yang, Q., J.E. Chauvin and Y. Herve. 1992. A study of factors affecting anther culture of cauliflower (Brassica oleracea var. botrytis). Plant Cell Tiss. Org. Cult. 28:289-296. https://doi.org/10.1007/BF00036126
  25. Zhang, J.Y., T.W. Bae, K.H. Boo, H.J. Sun, I.J. Song, C.H. Pham, M. Ganesan, D.H. Yang, H.G. Kang, S.M. Ko, K.Z. Riu, P.O. Lim and H.Y. Lee. 2011. Ginsenoside production and morphological characterization of wild ginseng (Panax ginseng Meyer) mutant lines induced by $\gamma$-irradiation $(^{60}Co)$ of adventitious roots. J. Ginseng Res. 35:283-293. https://doi.org/10.5142/jgr.2011.35.3.283

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