Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Genetic Diversity and Population Structure of Korean Mint Agastache rugosa (Fisch & Meyer) Kuntze (Lamiaceae) Using ISSR Markers

  • Kang, Man Jung (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Sundan, Suresh (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Lee, Gi An (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Ko, Ho Cheol (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Chung, Jong Wook (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Huh, Yun Chan (Vegetable Research Division, National Institute of Horticultural and Herbal Science, RDA) ;
  • Gwag, Jae Gyun (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Oh, Se Jong (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Kim, Yeon Gyu (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA) ;
  • Cho, Gyu Taek (National Agrobiodiversity Center, National Academy of Agricultural Science, RDA)
  • Received : 2012.11.12
  • Accepted : 2013.01.25
  • Published : 2013.06.30
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Abstract

Agastache rugosa, a member of the mint family (Labiatae), is a perennial herb widely distributed in East Asian countries. It is used in traditional medicine for the treatment of cholera, vomiting, and miasma. This study assessed the genetic diversity and population structures on 65 accessions of Korean mint A. rugosa germplasm based on inter simple sequence repeat (ISSR) markers. The selected nine ISSR primers produced reproducible polymorphic banding patterns. In total, 126 bands were scored; 119 (94.4%) were polymorphic. The number of bands generated per primer varied from 7 to 18. A minimum of seven bands was generated by primer 874, while a maximum of 18 bands was generated by the primer 844. Six primers (815, 826, 835, 844, 868, and 874) generated 100% polymorphic bands. This was supported by other parameters such as total gene diversity ($H_T$) values, which ranged from 0.112 to 0.330 with a mean of 0.218. The effective number of alleles ($N_E$) ranged from 1.174 to 1.486 with a mean value of 1.351. Nei's genetic diversity (H) mean value was 0.218, and Shannon's information index (I) mean value was 0.343. The high values for total gene diversity, effective number of alleles, Nei's genetic diversity, and Shannon's information index indicated substantial variations within the population. Cluster analysis showed characteristic grouping, which is not in accordance with their geographical affiliation. The implications of the results of this study in developing a strategy for the conservation and breeding of A. rugosa and other medicinal plant germplasm are discussed.

Keywords

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