Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Ethanol Extract of Dioscorea batatas Stimulates Procollagen Production and Reduces UVB-induced MMPs Activity in Skin

마 에탄올추출물의 피부 collagen 합성 촉진 및 MMPs 활성 억제효과

  • Kim, Dae Sung (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University) ;
  • Jeon, Byoung Kook (Department of Herbal Resources, Professional Graduate School of Korean Medicine, Wonkwang University) ;
  • Lim, Nan Young (Department of Beauty Design, College of Environmental Resources, Wonkwang University) ;
  • Mun, Yeun Ja (Department of Anatomy, College of Korean Medicine, Wonkwang University) ;
  • Lee, Young Eun (Department of Food and Nutrition, College of Environmental Resources, Wonkwang University) ;
  • Woo, Won Hong (Department of Anatomy, College of Korean Medicine, Wonkwang University)
  • 김대성 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 전병국 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 임난영 (원광대학교 뷰티디자인학과) ;
  • 문연자 (원광대학교 한의과대학 해부학교실) ;
  • 이영은 (원광대학교 생활과학대학 식품영양학과) ;
  • 우원홍 (원광대학교 한의과대학 해부학교실)
  • Received : 2012.11.06
  • Accepted : 2013.02.28
  • Published : 2013.04.25
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Abstract

Ultraviolet (UV) B irradiation induces the production of matrix metalloproteinases (MMPs), which are responsible for the degradation or synthesis inhibition of collagenous extracellular matrix in connective tissues, causing skin photoaging. In this study, we examined the inhibitory effect of MMP-1 expression of yam extract in tumor necrosis factor-alpha (TNF-${\alpha}$)-stimulated human dermal fibroblast neonatal (HDFn) cell and preventive effect of UVB-induced damage in hairless mice skin. The synthesis of procollagen and the release of MMP-1 in HDFn cells were measured by EIA kit and MMP-1 assay kit, respectively. UVB radiation was applied to the backs of the mice three times a week for 8 weeks. Mice were randomly divided into three groups, and were topical application with the Dioscorea batatas (DB, 6%) or vehicle. Reduction of TNF-${\alpha}$-induced procollagen synthesis was increased by DB (50 ug/ml), which was higher than positive control group (TGF-${\beta}$). Also, pre-treatment of HDFn cells with DB inhibited TNF-${\alpha}$-induced release of MMP-1. In vivo study, we found that preventive effect of DB against UV-induced epidermal thickness. DB suppressed the expression of MMP-3 and MMP-13 induced by UVB irradiation. Our results show that DB have preventive effect of UV-induced skin damage in hairless mice.

Keywords

References

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