The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Identification of Black Aspergillus Strains Isolated from Meju

메주에서 분리한 검은 Aspergillus 균주의 동정

  • Hong, Seung-Beom (Korean Agricultural Culture Collection, Agricultural Microbiology Div. National Academy of Agricultural Science, RDA) ;
  • Kim, Dae-Ho (Korean Agricultural Culture Collection, Agricultural Microbiology Div. National Academy of Agricultural Science, RDA) ;
  • Kim, Seon-Hwa (Korean Agricultural Culture Collection, Agricultural Microbiology Div. National Academy of Agricultural Science, RDA) ;
  • Bang, Narae (Korean Agricultural Culture Collection, Agricultural Microbiology Div. National Academy of Agricultural Science, RDA) ;
  • Kwon, Soon-Wo (Korean Agricultural Culture Collection, Agricultural Microbiology Div. National Academy of Agricultural Science, RDA)
  • 홍승범 (국립농업과학원 농업미생물과) ;
  • 김대호 (국립농업과학원 농업미생물과) ;
  • 김선화 (국립농업과학원 농업미생물과) ;
  • 방나래 (국립농업과학원 농업미생물과) ;
  • 권순우 (국립농업과학원 농업미생물과)
  • Received : 2013.06.11
  • Accepted : 2013.06.18
  • Published : 2013.06.30
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Abstract

Black Aspergillus is important fungus for oriental fermentation industry. Black Aspergillus was frequently isolated from Korean traditional Meju, a fermented soybean starting material for soy sauce and soybean paste. Thirty three strains were isolated from 98 finished Meju collected in various regions of Korea from 2008 to 2011, and 21 strains were isolated from in-process Meju at various farms from 2010 to 2011. The isolated black Aspergillus were identified using DNA sequences of partial ${\beta}$-tubulin and calmodulin genes. Of 54 black Aspergillus strains, 14 strains were identified as A. luchuensis and the others were composed of A. niger (n = 21), A. tubingensis (n = 10), and A. welwitschiae (n = 9).

검은 Aspergillus 속균은 동양의 발효산업에서 매우 중요한 곰팡이로서 식품산업에 관련된 검은 Aspergillus를 흑국균이라고 한다. 흑국균은 A. luchuensis, A. niger, A. tubingensis로 구성이 되어 있는데 이 중에서 A. luchuensis는 동양의 발효산업에서 매우 중요한 곰팡이로 알려져 있다. 우리나라 전통 된장과 간장의 주요 원료가 되는 메주에서도 검은 Aspergillus가 흔히 발견되었다. 33균주의 검은 Aspergillus가 2008년부터 2011년 사이에 전국적으로 수집한 98개의 완성메주로부터 분리되었고 추가로, 발효과정에 있는 메주로부터 검은 Aspergillus 21균주를 분리하였다. ${\beta}$-tubulin과 calmodulin 유전자 염기서열 분석을 통하여 이들을 동정한 결과 이들은 A. niger 21균주, A. luchuensis 14균주, A. tubingensis 10균주, A. welwitschiae 9균주로 동정되었다. 이 중 A. luchuensis는 생장이 활발하고 단백질, 지방, 탄수화물 분해력이 높은 것으로 조사되어 이들의 산업적 응용에 대한 추가의 연구가 필요한 것으로 사료된다.

Keywords

References

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