Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지
DOI QR코드

DOI QR Code

Isolation of Bacillus atrophaeus MPL-01 from A Wild Boar and Characterization of Its Antifungal Activity

멧돼지 대장으로부터 Bacillus atrophaeus MPL-01의 분리 및 항진균 활성의 특성

  • Received : 2013.05.31
  • Accepted : 2013.06.19
  • Published : 2013.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

A bacterial strain MPL-01 was isolated from the large intestine of a wild boar. The strain was shown to have morphological, physiological and biochemical characteristics, fatty acids composition typical of Bacillus. The 16S rRNA gene sequence showed that the isolate formed distinct phyletic line that was most closely related to this of Bacillus atrophaeus (99.99%). It was proposed that the strain is classified as B. atrophaeus MPL-01. The strain MPL-01 exhibited the strongest antifungal activity against Colletotrichum acutatum, the pathogen of anthracnose of chili peppers. The ethyl acetate extract of culture filtrate possessed not only the antifungal activity but also the bio-surfactant activity. Therefore, the strain MPL-01 could be a useful bacterium in the development of bio-control process against the pathogenic fungi.

멧돼지 대장에서 MPL-01 균주를 분리하여 균주의 형태학적, 생리 생화적 특성 및 지방산 조성을 분석하여 Bacillus임을 확인하였다. 16S rRNA 유전자 서열 분석 결과 MPL-01 균주는 Bacillus atrophaeus와 거의 일치하므로(99.99%) B. atrophaeus MPL-01로 명명하였다. MPL-01 균주는 고추 탄저병을 일으키는 Colletotrichum acutatum에 대하여 가장 강한 항진균 활성을 나타내었다. 배양액의 ethyl acetate 추출액에서 항진균 활성은 물론이고 계면활성도 확인하였다. 그러므로 B. atrophaeus MPL-01은 농작물 병원성 곰팡이 제어를 위한 bio-control 개발에서 유용하게 사용될 것이다.

Keywords

References

  1. Besson, F., Hourdou, M.L., and Michel, G. 1990. Studies on the biosynthesis of iturin, an antibiotic of Bacillus subtilis, and a lipopeptide containing beta-hydroxy fatty acids. Biochim. Biophys. Acta. 1036, 101-106. https://doi.org/10.1016/0304-4165(90)90020-W
  2. Besson, F. and Michel, G. 1990. Mycosubtilins B and C: minor antibiotics from mycosubtilin producer Bacillus subtilis. Microbios 62, 93-99.
  3. Bodour, A.A. and Miller-Maier, R.M. 1998. Application of a modified drop-collapse technique for surfactant quantitation and screening of biosurfactant producing microorganisms. J. Microbiol. Methods 32, 273-280. https://doi.org/10.1016/S0167-7012(98)00031-1
  4. Chun, J. and Bae, K.S. 2000. Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyrA gene sequences. Antonie van Leeuwenhoek 78, 123-127. https://doi.org/10.1023/A:1026555830014
  5. Eshita, S.M., Roberto, N.H., Beale, J.M., Mamiya, B.M., and Workman, R.F. 1995. Bacillomycin Lc, a new antibiotic of the iturin group: isolations, structures, and antifungal activities of the congeners. J. Antibiot. 48, 1240-1247. https://doi.org/10.7164/antibiotics.48.1240
  6. Hotta, K. 1996. Mechanism of multiple aminoglycoside resistance of kasugamycin-producing Streptomyces kasugaensis MB273 : involvement of two types of acetyltransferases in resistance to astromicin group antibiotics. J. Antibiot. (Tokyo) 49, 682-688. https://doi.org/10.7164/antibiotics.49.682
  7. Jang, Y.L. and Kim, Y.H. 2011. Biocontrol efficacies of Bacillus species against Cylindrocarpon destructans causing ginseng root rot. Plant Pathol. J. 27, 333-341. https://doi.org/10.5423/PPJ.2011.27.4.333
  8. Kermanshahi, R.K. and Peymanfar, S. 2012. Isolation and identification of Lactobacilli from cheese, yoghurt and silage by 16S rDNA gene and study of bacteriocin and biosurfactant production. Jundishapur J. Microbiol. 5, 528-532. https://doi.org/10.5812/jjm.3444
  9. Larsen, S.H., Berry, D.M., Paschal, J.W., and Gilliam, J.M. 1989. 5-Hydroxymethyl blasticidin S and blasticidin S from Streptomyces setonii culture A 83094. J. Antibiot. 42, 470-471. https://doi.org/10.7164/antibiotics.42.470
  10. Maget-Dana, R. and Peypoux, F. 1994. Iturins, a special class of pore-forming lipopeptides: biological and physicochemical properties. Toxicology 87, 151-174. https://doi.org/10.1016/0300-483X(94)90159-7
  11. Nakamura. L.K. 1989.Toxonomic relationship of black-pigmented Bacillus subtilis strains and a proposal for Bacillus atrophaeus sp. nov.. Int. J. Syst. Bacteriol. 39, 295-300. https://doi.org/10.1099/00207713-39-3-295
  12. Peypoux, F., Pommier, M.T., Marion, D., Ptak, M., Das, B.C., and Michel, G. 1986. Revised structure of mycosubtilin, a peptidolipid antibiotic from Bacillus subtilis. J. Antibiot. 39, 636-641. https://doi.org/10.7164/antibiotics.39.636
  13. Tenoux, I., Besson, F., and Michel, G. 1991. Studies on the antifungal antibiotics: bacillomycin D and bacillomycin D methylester. Microbios 67, 187-193.
  14. Tuleva, B.K., Ivanov, G.R., and Christovam N.E. 2002. Biosurfactant production by new Pseudomonas putida strain. Z. Naturforsch. 57, 356-360.
  15. Yu, G., Sinclair, J.B., Hartman, G.L., and Bertagnolli, B.L. 2002. Production of iturin A by Bacillus amyloliquefaciens suppressing Rhizoctonia solani. Soil Biol. Biochem. 34, 955-963. https://doi.org/10.1016/S0038-0717(02)00027-5
  16. Punja, Z.K. and Utkhede, R. 2003. Using fungi and yeasts to manage vegetable crop disease. Trends Biotechnol. 21, 400-407. https://doi.org/10.1016/S0167-7799(03)00193-8
  17. Zaragoza, A., Aranda, F.J., Espuny, M.J., Teruel, J.A., Marqués, A., Manresa, A., and Ortiz, A. 2010. Hemolytic activity of a bacterial trehalose lipid biosurfactant produced by Rhodococcus sp.: evidence for a colloid-osmotic mechanism. Langmuir 26, 8567-8572. https://doi.org/10.1021/la904637k