미생물학회지 (Korean Journal of Microbiology)

  • 제49권2호
  • /
  • Pages.146-149
  • /
  • 2013
  • /
  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지
DOI QR코드

DOI QR Code

서해안에서 분리한 비브리오균의 항생제 감수성 특성

Antibiotic Susceptibility of Vibrio spp. Isolated from West Sea

  • Kang, Chang-Ho (Department of Biological Engineering, Inha University) ;
  • Oh, Soo Ji (Department of Biological Engineering, Inha University) ;
  • So, Jae-Seong (Department of Biological Engineering, Inha University)
  • 투고 : 2013.04.16
  • 심사 : 2013.06.19
  • 발행 : 2013.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

비브리오균은 해양 및 하구 환경에서 상재하는 균으로 그람 음성 간균, 호염성, 비포자형성의 특징을 가지고 있다. 질병과 관련된 균주로는 Vibrio parahaemolyticus, Vibrio vulnificus, Vibrio cholera 등이 있다. 매년 어패류의 섭취 등을 통해 식중독 사고가 빈번하게 발생하고 있으며, 이에 본 연구에서는 서해안 6곳에서 패류를 채취하여 비브리오균의 분포도를 모니터링하고, 이들의 항생제 내성을 분석하였다. 총 120건의 패류를 TCBS 평판배지와 API 20E kit 기법으로 동정하였을 때 23.3%의 시료에서 비브리오균이 검출되었다. 이들 분리균주들의 항생제 내성을 16종의 항생제에 대해 분석한 결과 82.1%가 각각 vancomycin과 ampicillin에 대해 내성을 보였고, rifampin (71.4%)과 cephalothin (53.6%)에 대한 내성비율도 다른 항생제들에 비해 상대적으로 높은 것으로 나타났다. 분리균주들의 항생제 감수성은 chloramphenicol (92.9%), sulfamethoxazole/trimethoprim (92.9%)과 tetracycline (96.4%)에 대해 가장 높았다. 분리균주들의 약 71.4%가 vancomycin과 ampicillin을 포함하여 3개 이상의 항생제에 대해 다중내성을 보였다.

Bacteria of genus Vibrio are Gram-negative, curved, halophilic, nonspore-forming bacteria, autochthonous inhabitans of the marine and estuarine environments. Some of the Vibrio species such as V. parahaemolyticus, V. vulnificus, and V. cholerae are associated with human disease. Each year many people have been suffering from food-borne disease caused by the ingestion of seafood. In this study, we have monitored antibiotic resistance of this microorganism in 6 coastal areas of West Sea by sampling shellfish monthly. Vibrio spp. were detected from 23.3% of 120 samples analyzed using TCBS agar plates as well as API 20E kit. Among 16 antibiotics tested, resistance to vancomycin and ampicillin was observed in 82.1% of the isolates, and Vibrio spp. resistant to rifampin (71.4%) and cephalothin (53.6%) were also high. Most of the isolates were sensitive to chloramphenicol (92.9%), sulfamethoxazole/trimethoprim (92.9%), and tetracycline (96.4%). About 71.4% of the isolates showed multiple drug resistance toward 3 antibiotics including vancomycin and ampicillin.

키워드

참고문헌

  1. Bauer, H.W., Kirby, W.M.M., Sherris, J.C., and Turk, M. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45, 493-496. https://doi.org/10.1093/ajcp/45.4_ts.493
  2. Clinical Laboratory Standard Institute. 2007. Performance standards for antimicobial susceptibility testing: seventeenth informational supplement, M100-S17. Wayne, PA, USA.
  3. Ellingsen, A.B., Jorgensen, H., Wagley, S., Monshaugen, M., and Rorvik, L.M. 2008. Genetic diversity among Norwegian Vibrio parahaemolyticus. J. Appl. Microbiol. 105, 2195-2202. https://doi.org/10.1111/j.1365-2672.2008.03964.x
  4. Gaskins, H.R., Collier, C.T., and Anderson, D.B. 2002. Antibiotics as growth promotants: Mode of action. Anim. Biotechnol. 13, 29-42. https://doi.org/10.1081/ABIO-120005768
  5. Giguere, S., Prescott, J.F., Baggot, J.D., Walker, R.D., and Dowling, P.M. 2006. Antimicrobial theraphy in veterinary medicine, 4th Ed. Blackwell Publishing, Iowa, USA.
  6. Han, A.R., Yoon, Y.J., and Kim, J.W. 2012. Antibiotic resistance and plasmid profile of Vibrio parahaemolyticus strains isolated from Kyunggi-Incheon coastal area. Kor. J. Microbiol. 48, 22-28. https://doi.org/10.7845/kjm.2012.48.1.022
  7. Klonze, K.C., Cover, D.E., Hyman, F.N., and Mullen, R.C. 1994. Fatal gastroenteritis due to Vibrio fluvialis and nonfatal bacteremia due to Vibrio mimicus: unusual Vibrio infections in two patients. Clin. Infect. Dis. 19, 541-542. https://doi.org/10.1093/clinids/19.3.541
  8. Kümmerer, K. 2009. Antibiotics in the aquatic environment - A review - Part I. Chemosphere 75, 417-434. https://doi.org/10.1016/j.chemosphere.2008.11.086
  9. Lee, K. and Park, P. 2010. Antibiotic-resistance profiles and the identification of the ampicillin-resistance gene of Vibrio parahaemolyticus isolated from Seawater. Kor. J. Fish Aquat. Sci. 43, 637-641. https://doi.org/10.5657/kfas.2010.43.6.637
  10. Lesmana, M., Subekti, D.S., Tjaniadi, P., Simanjuntak, C.H., Punjabi, N.H., Campbell, J.R., and Oyofo, B.A. 2002. Spectrum of Vibrio species associated with acute diarrhea in North Jakarta, Indonesia. Diagn. Microbiol. Infect. Dis. 43, 291-297.
  11. Neela, F.A., Nonaka, L., and Suzuki, S. 2007. The diversity of multi-drug resistance profiles in tetracycline resistant Vibrio species isolated from coastal sediments and seawater. J. Microbiol. 45, 64-68.
  12. Oh, E.G., Son, K.T., Ha, K.S., Yoo, H.D., Yu, H.S., Shin, S.B., Lee, H.J., and Kim, J.H. 2009. Antimicrobial resistance of Vibrio strains from brackish water on the coast of Gyeongsangnamdo. Kor. J. Fish. Aquat. Sci. 42, 335-343. https://doi.org/10.5657/kfas.2009.42.4.335
  13. Son, K.T., Oh, E.G., Lee, T.S., Lee, H.J., Kim, P.H., and Kim, J.H. 2005. Antimicrobial susceptibility of Vibrio parahaemolyticus and Vibrio alginolyticus from fish farms on the southern coast of Korea. J. Kor. Fish. Soc. 38, 365-371. https://doi.org/10.5657/kfas.2005.38.6.365
  14. Son, J.C., Park, S.W., and Min, K.J. 2003. Environmental and antimicrobial characteristics of Vibrio spp. isolated from fish, shellfish, seawater and brackish water samples in Gyeongbuk eastern coast. Kor. J. Env. Hlth. 29, 94-102.
  15. Tollefson, L., Altekruse, S.F., and Potter, M.E. 1997. Therapeutic antibiotics in animal feeds and antibiotic resistance. Rev. Sci. Tech. 16, 709-715. https://doi.org/10.20506/rst.16.2.1054
  16. Urakawa, H., Yoshida, T., Nishimura, M., and Ohwada, K. 2000. Characterization of depth-related population variation in microbial communities of a coastal marine sediment using 16S rDNA-based approaches and quinone profiling. Environ. Microbiol. 2, 542-554. https://doi.org/10.1046/j.1462-2920.2000.00137.x
  17. Vaseeharan, B., Ramasamy, P., Murugan, T., and Chen, J.C. 2005. In vitro susceptibility of antibiotics against Vibrio spp. and Aeromonas spp. isolated from Penaeus monodon hatcheries and ponds. Int. J. Antimicrob. Agents 26, 285-291. https://doi.org/10.1016/j.ijantimicag.2005.07.005
  18. Zanetti, S., Spanu, T., Deriu, A., Romano, L., Sechi, L.A., and Fadda, G. 2001. In vitro susceptibility of Vibrio spp. isolated from the environment. Int. J. Antimicrob. Agents 17, 407-409. https://doi.org/10.1016/S0924-8579(01)00307-7