Preventive Nutrition and Food Science

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Hepatic Fibrosis Inhibitory Effect of Peptides Isolated from Navicula incerta on TGF-β Induced Activation of LX-2 Human Hepatic Stellate Cells

  • Kang, Kyong-Hwa (Marine Bioprocess Research Center, Pukyong National University) ;
  • Qian, Zhong-Ji (Oceanic Life Research Center, Chosun University) ;
  • Ryu, BoMi (Marine Bioprocess Research Center, Pukyong National University) ;
  • Karadeniz, Fatih (Marine Bioprocess Research Center, Pukyong National University) ;
  • Kim, Daekyung (Marine Bio Research Team, Korea Basic Science Institute (KBSI)) ;
  • Kim, Se-Kwon (Marine Bioprocess Research Center, Pukyong National University)
  • Received : 2013.05.07
  • Accepted : 2013.05.27
  • Published : 2013.06.30
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Abstract

In this study, novel peptides (NIPP-1, NIPP-2) derived from Navicula incerta (microalgae) protein hydrolysate were explored for their inhibitory effects on collagen release in hepatic fibrosis with the investigation of its underlying mechanism of action. TGF-${\beta}1$ activated fibrosis in LX-2 cells was examined in the presence or absence of purified peptides NIPP-1 and NIPP-2. Besides the mechanisms of liver cell injury, protective effects of NIPP-1 and NIPP-2 were studied to show the protective mechanism against TGF-${\beta}1$ stimulated fibrogenesis. Our results showed that the core protein of NIPP-1 peptide prevented fibril formation of type I collagen, elevated the MMP level and inhibited TIMP production in a dose-dependent manner. The treatment of NIPP-1 and NIPP-2 on TGF-${\beta}1$ induced LX-2 cells alleviated hepatic fibrosis. Moreover, ${\alpha}$-SMA, TIMPs, collagen and PDGF in the NIPP-1 treated groups were significantly decreased. Therefore, it could be suggested that NIPP-1 has potential to be used in anti-fibrosis treatment.

Keywords

References

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