Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Analysis of Genetic Relationship of Apple Varieties using Microsatellite Markers

Microsatellite 마커를 이용한 사과 품종 간 유전적 유연관계 분석

  • Hong, Jee-Hwa (Variety Testing Division, Korea Seed and Variety Service, Ministry for Food, Agriculture, Forestry and Fisheries) ;
  • Kwon, Yong-Sham (Variety Testing Division, Korea Seed and Variety Service, Ministry for Food, Agriculture, Forestry and Fisheries) ;
  • Choi, Keun-Jin (Variety Testing Division, Korea Seed and Variety Service, Ministry for Food, Agriculture, Forestry and Fisheries)
  • 홍지화 (농림수산식품부 국립종자원 재배시험과) ;
  • 권용삼 (농림수산식품부 국립종자원 재배시험과) ;
  • 최근진 (농림수산식품부 국립종자원 재배시험과)
  • Received : 2013.01.17
  • Accepted : 2013.05.30
  • Published : 2013.06.30
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Abstract

The objective of this study was to evaluate the suitability of microsatellite markers for variety identification in 42 apple varieties. For microsatellite analysis, 305 primer pairs were screened in 8 varieties and twenty six primer pairs showed polymorphism with clear band pattern and repetitive reproducibility. A total of 165 polymorphic amplified fragments were obtained in 42 varieties using 26 markers. Two to twelve alleles were detected for each locus with an average of 6.4 alleles per locus. A value of polymorphism information content (PIC) ranged from 0.461 to 0.849 with an average of 0.665. A total of 165 marker loci were used to calculate Jaccard's distance coefficients using unweighted pair-group method with arithmetical average (UPGMA) cluster analysis. Genetic distance of cluster ranged from 0.27 to 1.00. Analysis of genetic relationship revealed that these 26 microsatellite marker sets discriminated a total of 41 varieties except for 1 variety among 42 varieties. These markers will be utilized as molecular data in variety identification of apple.

본 연구는 microsatellite 마커를 이용하여 국립종자원 서부지원에 수집된 사과 42품종에 대한 품종 간 유전적 유연관계를 분석하였다. 사과 품종식별에 적합한 마커를 선정하기 위하여 8개 품종을 대상으로 총 305개의 마커를 분석하였다. 8개 품종 간에 다형성이 높고, 반복간 재현성이 있으며 밴드패턴이 선명한 26개의 마커를 최종 선발하여 42품종을 대상으로 분석하였을 때 총 165개의 대립유전자가 분석되었다. 대립유전자의 수의 분포는 2~12개를 나타내었으며, 마커당 평균 대립유전자의 수는 6.4개로 조사되었다. PIC 값은 0.461~0.849의 범위에 속하였으며 평균값은 0.665로 나타났다. 165개의 대립유전자를 Jaccard 방법에 의해 유사도를 산출하고 비가중 산술방식에 의해 집괴 분석한 결과 공시품종의 유전적 거리는 0.27~1.00의 범위를 나타내었고, 총 42품종 중 41품종은 microsatellite 마커의 유전자형에 의해 구분되었다. 본 연구결과는 사과 품종의 식별을 위한 분자생물학적 자료로 유용하게 활용될 것으로 사료된다.

Keywords

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