Journal of Korean Medicine for Obesity Research (한방비만학회지)

The Society of Korean Medicine for obesity Research (한방비만학회)
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Effect of Unfermented and Fermented Atractylodes macrocephalae on Gut Permeability and Lipopolysaccharide-Induced Inflammation

백출 및 발효백출의 장점막 투과성 개선 효과 및 항염증효과

  • Han, Kyungsun (Department of Oriental Rehabilitation Medicine, Dongguk University Ilsan Oriental Hospital) ;
  • Kim, Kicheol (Department of Oriental Rehabilitation Medicine, Dongguk University Ilsan Oriental Hospital) ;
  • Wang, Jinghua (Department of Oriental Rehabilitation Medicine, Dongguk University Ilsan Oriental Hospital) ;
  • Kim, Hojun (Department of Oriental Rehabilitation Medicine, Dongguk University Ilsan Oriental Hospital)
  • 한경선 (동국대학교 일산한방병원 한방재활의학과) ;
  • 김기철 (동국대학교 일산한방병원 한방재활의학과) ;
  • 왕경화 (동국대학교 일산한방병원 한방재활의학과) ;
  • 김호준 (동국대학교 일산한방병원 한방재활의학과)
  • Received : 2013.05.28
  • Accepted : 2013.06.04
  • Published : 2013.06.30
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Abstract

Objectives: The aim of this study is to investigate anti-imflammatory and protective effect for intestinal epithelial cells with Atractylodes macrocephae (AM), a traditional Korean Herbal medicine and fermented Atractylodes macrocephae (FAM) with Lactobacillus plantarum. Methods: HCT-116 and Raw 264.7 cells were used in this study. Using NO assay, we measured lipopolysaccharide (LPS)-induced anti-inflammatory effect. We measured permeability of intestinal epithelial cells with transepithelial electrical resistance and horseradish peroxide flux assay. Water soluble tetrazolium salt assay was used to see cell proliferation. All the results were presented in mean and standard deviation. We used Student's t-test for analyzing significance of results. Results: In Raw 264.7 cells NO production decreased 22.4% with pre-treatment of AM and FAM, especially with FAM in high concentration. In HCT-116 cells LPS-induced intestinal permeability had a protective effect with both AM and FAM, which was also tend to be proportional to the concentration. Cell viability increased up to 135.52% after treatment of high concentration of FAM in HCT-116, while there was no significant change in Raw 264.7 cells with herb treatments. Conclusions: These results show evidence that AM, especially fermented ones, significantly reduced intestinal membrane permeability. They also had a protective effect as well as an anti-inflammation effect for HCT-116 and Raw 264.7 cells. This suggest that FAM may be a therapeutic agent for Leaky gut syndrome by reducing intestinal permeability.

Keywords

References

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