Ingredients of Tyrosinase and Elastase Inhibitory Activity from Calyx of Diospyros kaki Thunberg

감꼭지의 Tyrosinase와 Elastase 저해 활성 성분

  • Cha, Bae Cheon (Department of Pharmaceutical Engineering, College of Health Sciences, Sangji University) ;
  • Lee, Eun Hee (Department of Pharmaceutical Engineering, College of Health Sciences, Sangji University)
  • 차배천 (상지대학교 보건과학대학 제약공학과) ;
  • 이은희 (상지대학교 보건과학대학 제약공학과)
  • Received : 2013.05.03
  • Accepted : 2013.05.22
  • Published : 2013.06.30

Abstract

This study was conducted to investigate glutathione S-transferase(GST) activity, tyrosinase inhibitory effect and elastase inhibitory effect in persimmon calyx(calyx of Diospyros kaki Thunberg) for screening of functional materials from natural products. As a result, EtOAc extract of persimmon calyx turned out to be having tyrosinase inhibitory effect and elastase inhibitory effect. The active constituents of tyrosinase and elastase inhibitory effect were isolated from EtOAc extract of persimmon calyx. Their structure of compounds were identified as ursolic acid and (-)-daucosterol by spectroscopic evidence, respectively.

Keywords

References

  1. Puizina-Ivic, N. (2008) Skin aging. Acta Dermatovenerol. Alp. Panonica Adriat. 17: 47-54.
  2. Iwata, M., Corn, T., Iwata, S., Evertt, M. A. and Fuller, B. B. (1990) The relationship between tyrosinase activity and skin color in human foreskins. J. Invest. Dermatol. 95: 9-15. https://doi.org/10.1111/1523-1747.ep12872677
  3. Gilcherst, B. A., Zhai, S., Eller, M. S., Yarosh, D. B. and Yaar. M. (1993) Treatment of human melanocytes and S91 melanoma cells with the DNA repair enzyme T4 endonuclease V enhances melanogenesis after ultraviolet irradiation. J. invest. Dermatol. 101: 666-672. https://doi.org/10.1111/1523-1747.ep12371673
  4. Lubart, R., Friedmann, H., Lavie, R., Longo, L., Jacobi, J., Baruchin, O. and Baruchin, A. M. (2007) A reasonable mechanism for visible light-induced skin rejuvenation. Lasers Med. Sci. 22: 1-3.
  5. Sa, Y. S., Kim, K. A. and Choi, H. S. (2003) Purification and characterization of anti-coagulant activity fraction from persimmon stem. J. Korean Soc. Food Sci. Nutr. 32: 1323-1327. https://doi.org/10.3746/jkfn.2003.32.8.1323
  6. Kim, H. J., Park, T. S., Jung, M. S. and Son, J. H. (2011) Study on the anti-oxidant and anti-inflammatory activities of sarcocarp and calyx of persimmon (Cheongdo Bansi). J. Appl. Biol. Chem. 54: 71-78. https://doi.org/10.3839/jabc.2011.013
  7. Angelucci, S., Sacchetta, P., Moio, P., Melino, S., Petruzzelli, R., Gervasi, P. and Di Ilio, C. (2000) Purification and characterization of glutathione transferases from the sea bass (Dicentrarchus labrax) liver. Arch. Biochem. Biophys. 373: 435-441. https://doi.org/10.1006/abbi.1999.1569
  8. Aroca, P., Urabe, K., Kobayashi, K., Taskamoto, K. and Hearing, V. J. (1993) Melanin biosynthesis patterns of following hormonal stimulation. J. Biol. Chem. 268: 25650-25655.
  9. Robert, L. and Homebck, W. (1989) Elastin and elastases. Vol. 2, 5762. CRC Press, Taylor and Francis Group.
  10. Habig, W. H., Pabst, M. J. and Jakoby, W. B. (1974) Glutathione S-transferases. J. Biol. Chem. 249: 7130-7139.
  11. Hearing Jr. V. J. (1987) Mammalian monophenol monooxygenase (Tyrosinase) : Purification, properties and reaction catalyzed. Methods Enzymol. 142: 154-165. https://doi.org/10.1016/S0076-6879(87)42024-7
  12. Lyu, S. Y., Moon, Y. S., Kwon, Y. J., Joo, H. J. and Park, W. B. (2003) Inhibition of porcine pancreatic elastase(PPE) by Korean mistletoe (Viscum album var. Coloratum) fractions. Nat. Prod. Sci. 9: 278-285.
  13. Hyun, Y. H., Koo, B. S., Song, J. E. and Kim, D. S. (2004) In food materials. 152-153. Hyungseul Publish, Daegu, Korea.
  14. Kameda, K., Takaku, T., Okuda, H., Kimura, Y., Okuda, T., Haitian, T., Agata, I. and Arichi, S. (1987) Inhibitory effects of various flavonoids isolated from leaves of persimmon on angiotensin- converting enzyme activity. J. Natl. Prod. 50: 680-683. https://doi.org/10.1021/np50052a017
  15. Okonogi, T., Hattori, Z., Ogiso, A. and Motsui, S. (1979) Detoxification by persimmon tannin of snake venoms and bacterial toxins. Toxicon. 17: 524-527. https://doi.org/10.1016/0041-0101(79)90287-3
  16. Hibasami, H., Achiwa, Y., Fujjkawa, T. and Komiya, T. (1996) Induction of programmed cell death (apoptosis) in human lymphoid leukemia cells by catechin compounds. Anticancer Res. 16: 1943-1946.
  17. Jin, Q., Jin, H. G., Shin, J. E., Choi, E. J. and Woo, E. R. (2010) Inhibition of IL-6 production in TNF-α stimulated MG-63 by the triterpenoids isolated from Aucuba japonica. Kor. J. Pharmacogn. 41: 97-102.
  18. Jaki, B. U., Franzblau, S. G., Chadwick, L. R., Lankin, D. C., Zhang, F., Wang, Y. and Pauli, G. F. (2008) Purity activity relationships of natural products: The case of anti-TB active ursolic acid. J. Nat. Prod. 71: 1742-1748. https://doi.org/10.1021/np800329j
  19. Lyu, H. N., Park, J. H., Kwak, H. Y., Lee, D. Y., Cho, J. G., Kim, S. Y., Kim, K. T. and Baek, N. I. (2009) Isolation of steroids from the Kalopanacis Cortex. J. Appl. Biol. Chem. 52: 41-44. https://doi.org/10.3839/jabc.2009.008
  20. Hong, Y., Qiao, Y., Lin, S., Jiang, Y. and Chen, Feng. (2008) Characterization of antioxidant compounds in Eriobotrya fragrans Champ leaf. Sci. Hortic. 118: 288-292. https://doi.org/10.1016/j.scienta.2008.06.018
  21. Lee, D. Y., Song, M. C., Yoo, J. S., Kim, S. H., Chung, I. S., Kim, D. K., Park, M. H., Kwon, B. M., Kim, S. Y. and Baek, N. I. (2006) Development of biologically active compounds from edible plant sources-17: Isolation of sterols from the fruits of Cornus kousa Burg. J. Korean Soc. Appl. Biol. Chem. 49: 82-85.
  22. 박은영 (2009) 황촉규 지상부의 항산화 및 항염 활성. 숙명여자대학 대학원 약학과, 박사학위논문.
  23. Sultana, N. and Lee, N. H. (2007) Antielastase and free radical scavenging activities of compounds from the stems of Cornus kousa. Phytother. Res. 21: 1171-1176. https://doi.org/10.1002/ptr.2230