Mass Spectrometry Letters

Korean Society for Mass Spectrometry (한국질량분석학회)
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Enhanced Detection of Glycans by MALDI-TOF Mass Spectrometry Using a Binary Matrix of 2,5-Dihydroxybenzoic Acid and 2,6-Dihydroxybenzoic Acid

  • Kim, Yunjin (Department of Chemistry, Chungnam National University) ;
  • Kim, Taehee (Department of Chemistry, Chungnam National University) ;
  • Lee, Jihyeon (Department of Chemistry, Chungnam National University) ;
  • Im, Haeju (Department of Chemistry, Chungnam National University) ;
  • Kim, Jeongkwon (Department of Chemistry, Chungnam National University)
  • Received : 2013.06.23
  • Accepted : 2013.06.27
  • Published : 2013.06.01
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Abstract

Glycans released from ovalbumin by PNGase F were analyzed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry using three different dihydroxybenzoic acid (DHB) matrix systems: 2,5-DHB, 2,6-DHB, and a 2,5-DHB/2,6-DHB binary matrix. Relative to the results obtained with the single-component matrices (2,5-DHB or 2,6-DHB), the 2,5-DHB/2,6-DHB binary matrix boasted lower background noise and higher sensitivity. A total of 16 glycan peaks were observed using the 2,5-DHB/2,6-DHB binary matrix, while only 10 and 9 glycan peaks were observed using the 2,5-DHB and 2,6-DHB matrices, respectively.

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References

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