Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Classification and Comparative Analysis of the Contents of Acorus species and Anemone altaica by UPLC-PDA Analysis

UPLC-PDA를 이용한 창포류의 분류 및 함량 분석

  • Jo, Ji Eun (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Lee, A Yeong (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Hyo Seon (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Moon, Byeong Cheol (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Ji, Yunui (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Chun, Jin Mi (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine) ;
  • Kim, Ho Kyoung (Basic Herbal Medicine Research Group, Korea Institute of Oriental Medicine)
  • 조지은 (한국한의학연구원 한약기초연구그룹) ;
  • 이아영 (한국한의학연구원 한약기초연구그룹) ;
  • 김효선 (한국한의학연구원 한약기초연구그룹) ;
  • 문병철 (한국한의학연구원 한약기초연구그룹) ;
  • 지윤의 (한국한의학연구원 한약기초연구그룹) ;
  • 천진미 (한국한의학연구원 한약기초연구그룹) ;
  • 김호경 (한국한의학연구원 한약기초연구그룹)
  • Received : 2012.12.20
  • Accepted : 2013.04.15
  • Published : 2013.06.30
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Abstract

A quantitative method using ultra performance liquid chromatography with a photodiode array detector (UPLCPDA) was established for the analysis of 2 major plant metabolites: ${\beta}$-asarone and ${\alpha}$-asarone from Acorus gramineus, A. tatarinowii, A. calamus and Anemone altaica, and their contents are compared with other herbs of Acorus species. The method was validated according to the International Conference on harmonization (ICH) guideline for validation of analytical procedures with respect to precision, accuracy, and linearity. The average content of ${\beta}$-asarone in Acorus gramineus was significantly higher than that in others, with the second highest concentration observed in A. tatarinowii, and only a trace amounts found in A. calamus and Anemone altaica. In contrast, the average content of ${\alpha}$-asarone in A. calamus was the highest, followed by that in Acorus gramineus and A. tatarinowii. principle component analysis (PCA) confirmed that ${\beta}$-asarone and ${\alpha}$-asarone content differed among the species. These results suggest that this UPLC-PDA method can be considered as good quality control criteria for Acorus gramineus.

본 연구에서는 UPLC를 이용하여 석창포의 기원종을 비교하기 위하여 4종 창포류(A. gramineus, A. tatarinowii, A. calamus, Anemone altaica)의 ${\alpha}$-asarone과 ${\beta}$-asarone의 함량 분석을 실시하였고, 분석법 validation은 ICH 지침에 따라 검증하였다. 창포류 4종에 대한 함량분석에서는 모든 시료에 대해 2% 이하의 상대 표준편차를 나타내었고, ${\beta}$-asarone의 함량은 석창포, 중국석창포, 창포, 구절창포 순으로 나타났으며, ${\alpha}$-asarone은 창포, 중국 석창포, 석창포 순으로 나타났다. 특히, 구절창포에서는 ${\beta}$-asarone이 미량이었고, ${\alpha}$-asarone는 검출되지 않았다. PCA 분석에서도 4종의 창포류가 네 그룹으로 나누어 지는 것을 확인할 수 있었다. 이와 같이, 본 실험 결과를 통하여 석창포, 중국 석창포, 창포 및 구절창포를 명확히 구별할 수 있었고, 석창포의 품질평가에 응용할 수 있을 것이라 기대된다.

Keywords

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