Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Development and Application of DNA Analysis Method for Identificaion of Main Ingredients in Starch

전분의 주원료 판별을 위한 유전자 분석법 개발 및 적용

  • Park, Yong-Chjun (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety) ;
  • Kim, Mi-Ra (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety) ;
  • Kim, Yong-Sang (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety) ;
  • Lee, Ho-Yeon (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety) ;
  • Kim, Kyu-Heon (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety) ;
  • Lee, Jae-Hwang (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety) ;
  • Kim, Jae-I (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety) ;
  • Lee, Sang-Jae (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety) ;
  • Lee, Hwa-Jung (New Hazardous Substance Team, Food Safety Evaluation Department, National Institute of Food & Drug Safety Evaluation, Ministry of Food & Drug Safety)
  • 박용춘 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 김미라 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 김용상 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 이호연 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 김규헌 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 이재황 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 김재이 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 이상재 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀) ;
  • 이화정 (식품의약품안전처 식품의약품안전평가원 식품위해평가부 신종유해물질팀)
  • Received : 2013.03.29
  • Accepted : 2013.05.29
  • Published : 2013.06.30
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Abstract

Identification of main ingredients in starches has been investigated using physicochemical analysis method mainly. However, physicochemical properties such as particle size have limitations in determining the differences among mixed starches. Therefore, we developed a molecular biological method to identify materials used in starch, as a sample, 11 kinds of starches including sweet potato starch, potato starch, corn starch, and tapioca starch. DNeasy plant mini kit, magnetic DNA purification system, and CTAB methods were used to extract DNA from samples. After gene extraction, whole genome amplification (WGA) was performed to amplify the extracted DNA. Species-specific primers were used as followings: ib-286-F/ib-286-R (105 bp), Pss 01n-5'/Pss 01n-3' (216 bp), SS11b 3-5'/SS11b 3-3' (114 bp), and SSRY26-F/SSRY26-R (121 bp) gene for sweet potato, potato, corn, and tapioca, respectively. In this study, we could confirm the main ingredients using WGA and PCR method.

전분의 사용원료를 확인하는 방법은 전분입자의 크기 또는 형태 등으로 분류하는 이화학적인 방법이 연구되었으나 원료별 또는 동일한 원료라도 품종에 따른 차이점으로 인하여 명확하게 확인하기 어려운 단점이 있어 유전자분석법을 시도하였다. 시료는 고구마 전분, 감자 전분, 옥수수 전분 및 타피오카 전분 등 총 11종을 사용하였으며, 유전자추출은 DNeasy plant mini 키트, magnetic DNA purification system 및 CTAB 방법으로 하였으며 추출유전자의 증폭을 위하여 WGA 키트로 처리하였다. 그리고 고구마, 감자, 옥수수 및 타피오카 검출을 위한 유전자 부위는 SSR (simple sequence repeat, ib-286-F/ib-286-R), 자당합성효소(potato sucrose synthase, Pss 01n-5'/Pss 01n-3'), 전분합성효소(starch synthase, SSllb 3-5'/SSllb 3-3') 및 SSR (SSRY26-F/SSRY26-R)를 각각 사용하였다. 그 결과 대부분의 경우 WGA를 처리한 경우에는 사용원료의 확인이 가능하였다.

Keywords

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