Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
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Anti-diabetic Effects of Isaria tenuipes in OLETF Rats as an Animal Model of Diabetes Mellitus Type II

제 2형 당뇨모델 OLETF 랫드에서 동충하초의 항당뇨 효과

  • Seo, Dong-Seok (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Kang, Jong-Koo (College of Veterinary Medicine and Research Institute of Veterinary Medicine, Chungbuk National University) ;
  • Jeong, Mi-Hye (Division of Agro-Materials Safety Evaluation Department of Crop Life Safety National Academy of Agricultural Science) ;
  • Kwon, Min (Shinsung University) ;
  • Park, Cheol-Beom (Hoseo University)
  • 서동석 (충북대학교 수의과대학 실험동물의학교실) ;
  • 강종구 (충북대학교 수의과대학 실험동물의학교실) ;
  • 정미혜 (농촌진흥청) ;
  • 권민 (신성대학교) ;
  • 박철범 (호서대학교)
  • Received : 2012.12.21
  • Accepted : 2013.05.10
  • Published : 2013.06.30
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Abstract

We evaluated the anti-diabetic effects of Isaria tenuipes in diabetes mellitus type 2. For the experiments, the diabetic animal model OLETF rats were divided to 4 groups: Isaria tenuipes was administered mixed with the high fat diet 45% at dose levels of 0.0%, 0.1%, 1.0%, and 5.0% for 4 weeks. All animals have free access to water and high fat diet 45%. The diabetic clinical markers, including clinical signs, body weight and food intake, organ weights, blood glucose level, insulin level and HOMA-IR index, oral glucose tolerance test, GLUT4 mRNA and protein were measured at a time. After administration for 4 weeks, the blood glucose levels, insulin levels and HOMA-IR index of test groups were decreased compared with control group in dose-dependent manner. The body weight and diet consumptions were reduced in control group at 4 weeks. The treatments of Isaria tenuipes also showed high expression of GLUT4 mRNA and protein in the muscle of OLETF rats. The results suggest that Isaria tenuipes has anti-hyperglycemic effect attenuating blood glucose in the animal model of type 2 diabetes and might be useful as a functional diet for human diabetic diseases.

본 연구는 동충하초(Isaria tenuipes)를 유전적 당뇨 질환 동물모델인 OLETF 랫드에서 4주간 경구 투여하여 항당뇨 효과에 대한 연구결과로써, OLETF 랫드 동충하초(Isaria tenuipes) 투여군은 당뇨대조군과 비교하여 투여 4주에는 사료 섭취량과 체중이 증가하였다. 이는 동충하초(Isaria tenuipes)를 섭취한 OLETF 랫드는 고지방식 사료만 섭취한 OLETF 랫드에 비해 당뇨 유발이 개선된 결과로 판단된다. 혈중 포도당 농도, 인슐린 농도 및 인슐린 저항성 지표인 HOMA-IR 수치가 용량 의존적으로 감소하였다. 또한 GLUT4 유전자 발현을 측정한 결과 대조군에 비해 동충하초(Isaria tenuipes) 투여군의 mRNA 및 단백질이 용량 의존적으로 증가하는 경향을 보였다. 따라서, 동충하초(Isaria tenuipes)의 섭취가 제2형 당뇨에서 인슐린의 감수성이나 저항성에 영향을 미치고, 혈당을 강화시키는 것으로 사료되므로 당뇨병환자의 혈당저하에 기여할 것이라 기대한다. 이에 본 연구는 천연물질을 이용한 당뇨병의 예방 및 치료에 동충하초(Isaria tenuipes)를 사용할 수 있는 이론적 토대를 제공할 것으로 생각된다.

Keywords

References

  1. 통계청: 사망원인통계연보 2004. 대전, 통계청 (2005).
  2. Beak, S.H.: Prevention of type 2 diabetes. Medical postgraduates 3, 128-131 (2007).
  3. Holman, R.R., Bethel, M.A., Matthew, D.R. and Neil, H.A.: 10 year follow-up of intensive glucose control in type 2 diabetes. N. Eng. J. Med. 359, 1557-1589 (2008).
  4. Tapsell, L.C., Hemphill, I., Cobiac, L., Patch, C.S., Sullivan, D.R., Fenech, M., Roodenrys, S., Keogh, J.B., Clifton, P.M., Williams, P.G., Fazio, V.A. and Inge, K.E.: Health benefits of herbs and spices: the past, the present, the future. Med. J. Aust. 185, S4-24 (2006).
  5. Shokuhin to Kaihatsu Henshubu: Market trend of health foods and health ingredients. Shokuhin to Kaihatsu 35, 18-34 (2000).
  6. Ahn Y.J., Park S.J., Lee S.G., Shin S.C. and Choi D.H.: Cordycepin: selective growth inhibitor derived from liquid culture of Cordyceps militaris against Clostridium spp. J Agric Food Chem. 48(7), 2744-2748 (2000). https://doi.org/10.1021/jf990862n
  7. Zhao-Long W., Xiao-Xia W. and Wei-Ying C.: Inhibitory effect of Cordyceps sinensis and Cordyceps militaris on human glomerular mesangial cell proliferation induced by native LDL. Cell Biochem Funct. 18(2), 93-97 (2000). https://doi.org/10.1002/(SICI)1099-0844(200006)18:2<93::AID-CBF854>3.0.CO;2-#
  8. Ikumoto T., Sasaki S., Namba H., Toyama R., Moritoki H. and Mouri T.: Physiologically active compounds in the extracts from tochukaso and cultured mycelia of Cordyceps and Isaria. Yakugaku Zasshi. 111(9), 504-509 (1991). https://doi.org/10.1248/yakushi1947.111.9_504
  9. Uehara Y., Hirawa N., Numabe A., Kawabata Y., Nagoshi H., Negoro H., Fujiwara S., Gomi T., Ikeda T., Goto A. and Omata M.: Angiotensin-Converting Enzyme Inhibition Delays Onset of Glucosuria With Regression of Renal Injuries in Genetic Rat Model of Non-Insulin-Dependent Diabetes Mellitus. J. Cardiovasc. Pharmacol. Ther. 3, 327-336 (1998). https://doi.org/10.1177/107424849800300408
  10. Koga K., Yamagishi S., Takeuchi M., Inagaki Y., Amano S., Okamoto T., Saga T., Makita Z. and Yoshizuka M.: CS-886, a new angiotensin II type 1 receptor antagonist, ameliorates glomerular anionic site loss and prevents progression of diabetic nephropathy in Otsuka Long-Evans Tokushima fatty rats. Mol. Med. 8, 591-599 (2002).
  11. Okada M., Takemura T., Yanagida H., Yoshioka K.: Response of mesangial cells to low-density lipoprotein and angiotensin II in diabetic (OLETF) rats. Kidney Int. 61, 113-124 (2002). https://doi.org/10.1046/j.1523-1755.2002.00107.x
  12. Hong I.P., Kang P.D., Kim K.Y., Lee M.Y., Choi Y.S., Kim N.S., Kim H.K., Nam S.H., and Lee K.G.: Synnemata Production by Isaria tenuipes using Colored Cocoon Silkworm, Golden Silk. Int. J. Indust. Entomol. 22(1), 1-4 (2011). https://doi.org/10.7852/ijie.2011.22.1.1
  13. Caisheng L.U., Rengao Y.E., Qiongqiong Y., Xiao Y., Huiqun L.I. and Youji L.I.: Gene expression of glucose transorters and its regulation by gluicose in mesothelial cells. Chiness Med. J. 114, 447-480 (2001).
  14. Mogensen, C.E. and Anderson M.J.F.: Increase kidney size and glomerulal filtration rate in early juenile diabetes. Diabetes 22, 706-712 (1973). https://doi.org/10.2337/diab.22.9.706
  15. Gallaher, D.D., Casallany, A.S., Shoeman, D.W. and Olson, J.M.: Diabetes increases excretion of urinary malonaldehyde conjugates in rats. Lipids 28, 663-666 (1993). https://doi.org/10.1007/BF02536063
  16. Shon, K.H., Kim, S.H. and Choi, J.W.: Pretreatment with nicotinamide to prevent the pancreatic enzymes changes by streptozotocin in rats. J. Kor. Soc. Food Nutr. 21, 117-123 (1992).
  17. Niall, M.G., Rosaleen, A.M., Daphne, O., Patrick, B.C., Alan, H.J. and Gerald, H.T.: Cholesterol metabolism in alloxaninduced diabetic rabbits. Diabetes 39, 626-636 (1990). https://doi.org/10.2337/diabetes.39.5.626
  18. Wiederkehr, A. and Wollheim, C.B.: Minireview: implication of mitochondria in insulin secretion and action. Endocrinology 147, 2643-2649 (2006). https://doi.org/10.1210/en.2006-0057
  19. Holman, G.D. and Kasuga, M.: From receptor to transporter: insulin signaling to glucose transport. Diabetologia 40, 991-1003 (1997). https://doi.org/10.1007/s001250050780