Asian-Australasian Journal of Animal Sciences

  • 제26권8호
  • /
  • Pages.1080-1088
  • /
  • 2013
  • /
  • 1011-2367(pISSN)
  • /
  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Kisspeptin-10 Enhanced Egg Production in Quails Associated with the Increase of Triglyceride Synthesis in Liver

  • Wu, J. (Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University) ;
  • Fu, W. (Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University) ;
  • Huang, Y. (Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University) ;
  • Ni, Y. (Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University) ;
  • Zhao, R. (Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University)
  • 투고 : 2013.01.06
  • 심사 : 2013.03.18
  • 발행 : 2013.08.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Our previous results showed that kisspeptin-10 (Kp-10) injections via intraperitoneal (i.p.) once daily for three weeks notably promoted the egg laying rate in quails. In order to investigate the mechanism behind the effects of Kp-10 on enhancing the egg laying rate in birds, this study focused on the alternations of lipids synthesis in liver after Kp-10 injections. 75 female quails (22 d of age) were allocated to three groups randomly, and subjected to 0 (control, Con), 10 nmol (low dosage, L) and 100 nmol (high dosage, H) Kp-10 injections via i.p. once daily for three weeks, respectively. At d 52, quails were sacrificed and sampled for further analyses. Serum $E_2$ concentration was increased by Kp-10 injections, and reached statistical significance in H group. Serum triglyceride (TG) concentrations were increased by 46.7% in L group and 36.8% in H group, respectively, but did not reach statistical significance, and TG contents in liver were significantly elevated by Kp-10 injections in a dose-dependent manner. Serum total cholesterol (Tch) concentrations significantly decreased in H group, while in H group the hepatic Tch content was markedly increased. The level of non-esterified fatty acid (NEFA), apolipoprotein A1 and B (apoA1 and apoB) were not altered by Kp-10 injections. The genes expression of sterol regulatory element binding protein-1 (SREBP-1), fatty acid synthetase (FAS), apolipoprotein VLDL-II (apoVLDL-II), cholesterol $7{\alpha}$-hydroxylase (CYP7A1) and vitellogenin II (VTG-II) were significantly up-regulated by high but not low dosage of Kp-10 injection compared to the control group. However, the expression of SREBP-2, acetyl-CoA carboxylase ($ACC_{\alpha}$), malic enzyme (ME), stearoyl-CoA (${\Delta}9$) desaturase 1 (SCD1), apolipoprotein A1 (apoA1), fatty acid binding protein 2 (FABP2), 3-hydroxyl-3-methyl glutaryl-coenzyme A reductases (HMGCR), estrogen receptor ${\alpha}$, ${\beta}$($ER{\alpha}$ and ${\beta}$) mRNA were not affected by Kp-10 treatment. In line with hepatic mRNA abundance, hepatic SREBP1 protein content was significantly higher in H group. Although the mRNA expression was not altered, the content of $ER{\alpha}$ protein in liver was also significantly increased in H group. However, SREBP-2 protein content in liver was not changed by Kp-10 treatment. In conclusion, exogenous Kp-10 consecutive injections during juvenile stage significantly advanced the tempo of egg laying in quails, which was associated with the significant elevation in hepatic lipids synthesis and transport.

키워드

참고문헌

  1. Akazome, Y., S. Kanda, K. Okubo, and Y. Oka. 2010. Functional and evolutionary insights into vertebrate kisspeptin systems from studies of fish brain. J. Fish Biol. 76:161-182. https://doi.org/10.1111/j.1095-8649.2009.02496.x
  2. Bujo, H., M. Hermann, K. A. Lindstedt, J. Nimpf, and W. J. Schneider. 1997. Low density lipoprotein receptor gene family members mediate yolk deposition. J. Nutr. 127:801-804.
  3. Charlier, T. D., A. E. Newman, S. A. Heimovics, K. W. Po, C. J. Saldanha, and K. K. Soma. 2011. Rapid effects of aggressive interactions on aromatase activity and oestradiol in discrete brain regions of wild male white-crowned sparrows. J. Neuroendocrinol. 23:742-753. https://doi.org/10.1111/j.1365-2826.2011.02170.x
  4. Clarkson, J., and A. E. Herbison. 2006. Postnatal development of kisspeptin neurons in mouse hypothalamus; sexual dimorphism and projections to gonadotropin-releasing hormone neurons. Endocrinology 147:5817-5825. https://doi.org/10.1210/en.2006-0787
  5. Codina-Salada, J., J. P. Moore, and L. Chan. 1983. Kinetics of primary and secondary stimulation of the mRNA for APOVLDL-II, a major yolk protein, in the cockerel liver by estrogen. Endocrinology 113:1158-1160. https://doi.org/10.1210/endo-113-3-1158
  6. d'Anglemont de, T. X., L. A. Fagg, J. P. C. Dixon, K. Day, H. G. Leitch, A. G. Hendrick, D. Zahn, I. Franceschini, A. Caraty, M. B. Carlton, S. A. Aparicio, and W. H. Colledge. 2007. Hypogonadotropic hypogonadism in mice lacking a functional Kiss1 gene. Proc. Natl. Acad. Sci. USA. 104:10714-10719. https://doi.org/10.1073/pnas.0704114104
  7. de Roux, N. 2003. Hypogonadotropic hypogonadism due to loss of function of the KiSS1-derived peptide receptor GPR54. Proc. Natl. Acad. Sci. USA. 100:10972-10976. https://doi.org/10.1073/pnas.1834399100
  8. Gupta, S., R. U. Pathak, and M. S. Kanungo. 2006. DNA methylation induced changes in chromatin conformation of the promoter of the vitellogenin II gene of Japanese quail during aging. Gene 377:159-168. https://doi.org/10.1016/j.gene.2006.04.020
  9. Gous, R. M., G. D. Bradford, S. A. Johnson, and T. R. Morris. 2000. Effect of age of release from light or food restriction on age at sexual maturity and egg production of laying pullets. Br. Poult. Sci. 41:263-271. https://doi.org/10.1080/713654932
  10. Han, S. K., M. L. Gottsch, K. J. Lee, S. M. Popa, J. T. Smith, S. K. Jakawich, D. K. Clifton, R. A. Steiner, and A. E. Herbison. 2005. Activation of gonadotropin-releasing hormone neurons by kisspeptin as a neuroendocrine switch for the onset of puberty. J. Neurosci. 25:11349-11356. https://doi.org/10.1523/JNEUROSCI.3328-05.2005
  11. Hermier, D. 1997. Lipoprotein metabolism and fattening in poultry. J. Nutr. 127:805-808.
  12. Keen, K. L., F. H. Wegner, S. R. Bloom, M. A. Ghatei, and E. Terasawa. 2008. An increase in kisspeptin-54 release occurs with the pubertal increase in luteinizing hormone-releasing hormone-1 release in the stalk-median eminence of female rhesus monkeys in vivo. Endocrinology 149:4151-4157. https://doi.org/10.1210/en.2008-0231
  13. Kotani, M., M. Detheux, A. Vandenbogaerde, D. Communi, J. M. Vanderwinden, E. Le Poul, S. Brezillon, R. Tyldesley, N. Suarez-Huerta, F. Vandeput, C. Blanpain, S. N. Schiffmann, G. Vassart, and M. Parmentier. 2001. The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands of the orphan G protein-coupled receptor GPR54. J. Biol. Chem. 276:34631-34636. https://doi.org/10.1074/jbc.M104847200
  14. Le Menn, F., H. Rochefort, and M. Garcia. 1980. Effect of androgen mediated by the estrogen receptor of fish liver: vitellogenin accumulation. Steroids 35:315-328. https://doi.org/10.1016/0039-128X(80)90044-6
  15. Navarro, V. M. 2005. Effects of KiSS-1 peptide, the natural ligand of GPR54, on follicle-stimulating hormone secretion in the rat. Endocrinology 146:1689-1697. https://doi.org/10.1210/en.2004-1353
  16. Ni, Y. D., Y. B. Huang, Y. Q. Xiao, J. Wu, F. Y. Qian, R. Grossmann, and R. Q. Zhao. 2012. Effects of repeated injection of kisspeptin-10 on the initiation of egg-laying in juvenile quail. Anim. Reprod. Sci. 134:203-209. https://doi.org/10.1016/j.anireprosci.2012.08.022
  17. Oakley, A. E., D. K. Clifton, and R. A. Steiner. 2009. Kisspeptin signaling in the brain. Endocr. Rev. 30:713-743. https://doi.org/10.1210/er.2009-0005
  18. Ottinger, M. A., M. Abdelnabi, Q. Li, K. Chen, N. Thompson, N. Harada, C. Viglietti-Panzica, and G. C. Panzica. 2004. The Japanese quail a model for studying reproductive aging of hypothalamic systems. Exp. Gerontol. 39:1679-1693. https://doi.org/10.1016/j.exger.2004.06.021
  19. Richards, M. P., S. M. Poch, C. N. Coon, R. W. Rosebrough, C. M. Ashwell, and J. P. McMurtry. 2003. Feed restriction significantly alters lipogenic gene expression in broiler breeder chickens. J. Nutr. 133:707-715.
  20. Roa, J., J. M. Castellano, V. M. Navarro, D. J. Handelsman, L. Pinilla, and M. Tena-Sempere. 2009. Kisspeptins and the control of gonadotropin secretion in male and female rodents. Peptides 30:57-66. https://doi.org/10.1016/j.peptides.2008.08.009
  21. Saldanha,C. J., B. J. Walters, and G. S. Fraley. 2010. Neurons that co-localize aromatase-and kisspeptin-like immunoreactivity may regulate the HPG axis of the Mallard drake (Anas platyrhynchos). Gen. Comp. Endocrinol. 166:606-613. https://doi.org/10.1016/j.ygcen.2010.01.007
  22. Seminara, S. B., S. Messager, E. E. Chatzidaki, R. R. Thresher, J. S. Acierno, J. K. Shagoury, Y. Bo-Abbas, W. Kuohung, K. M. Schwinof, A. G. Hendrick, D. Zahn, J. Dixon, U. B. Kaiser, S. A. Slaugenhaupt, J. F. Gusella, S. O'Rahilly, M. B. Carlton, W. F. Crowley, S. A. Aparicio, and W. H. Colledge. 2003. The GPR54 gene as a regulator of puberty. N. Engl. J. Med. 349:1614-1627. https://doi.org/10.1056/NEJMoa035322
  23. Smith, J. T., H. M. Dungan, E. A. Stoll, M. L. Gottsch, R. E. Braun, S. M. Eacker, D. K. Clifton, and R. A. Steiner. 2005. Differential regulation of KiSS-1 mRNA expression by sex steroids in the brain of the male mouse. Endocrinology 146:2976-2984. https://doi.org/10.1210/en.2005-0323
  24. Tata, J. R. 1976. The expression of the vitellogenin gene. Cell 9:1-14. https://doi.org/10.1016/0092-8674(76)90047-7
  25. Um, H. N., J. M. Han, J. I. Hwang, S. I. Hong, H. Vaudry, and J. Y. Seong. 2010. Molecular coevolution of kisspeptins and their receptors from fish to mammals. Ann. N.Y. Acad. Sci. 1200: 67-74. https://doi.org/10.1111/j.1749-6632.2010.05508.x
  26. van Aerle, R., P. Kille, A. Lange, and C. R. Tyler. 2008. Evidence for the existence of a functional Kiss1/Kiss1 receptor pathway in fish. Peptides 29:57-64. https://doi.org/10.1016/j.peptides.2007.10.018
  27. Wallace, R. A., and K. Selman. 1985. Major protein changes during vitellogenesis and maturation of Fundulus oocytes. Dev. Biol. 110:492-498. https://doi.org/10.1016/0012-1606(85)90106-X
  28. Walzem, R. L., R. J. Hansen, D. L. Williams, and R. L. Hamilton. 1999. Estrogen induction of VLDLy assembly in egg-laying hens. J. Nutr. 129:467-472.
  29. Wingfield, J. C. 2005. Historical contributions of research on birds to behavioral neuroendocrinology. Horm. Behav. 48:395-402. https://doi.org/10.1016/j.yhbeh.2005.06.003
  30. Wilson, S. B., D. W. Back, S. M. Morris, J. Swierczynski, and A. G. Goodridge. 1986. Hormonal regulation of lipogenic enzymes in chick embryo hepatocytes in culture. Expression of the fatty acid synthase gene is regulated at both translational and pretranslational steps. J. Biol. Chem. 261:15179-15182.
  31. Xiao, Y., Y. D. Ni, Y. B. Huang, J. Wu, R. Grossmann, and R. Q. Zhao. 2011. Effects of kisspeptin-10 on progesterone secretion in cultured chicken ovarian granulosa cells from preovulatory $(F(_1)-F(_3))$ follicles. Peptides 32:2091-2097. https://doi.org/10.1016/j.peptides.2011.09.001
  32. Xu, Z. R., M. Q. Wang, H. X. Mao, X. A. Zhan, and C. H. Hu. 2003. Effects of L-carnitine on growth performance, carcass composition, and metabolism of lipids in male broilers. Poult. Sci. 82:408-413. https://doi.org/10.1093/ps/82.3.408

피인용 문헌

  1. The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages vol.2016, pp.1687-8345, 2016, https://doi.org/10.1155/2016/6790794
  2. Origin and Evolution of the Neuroendocrine Control of Reproduction in Vertebrates, With Special Focus on Genome and Gene Duplications vol.100, pp.2, 2020, https://doi.org/10.1152/physrev.00009.2019