Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Changes in Concentrations of Reproductive Hormones during Estrus Synchronization in Dairy Heifers

젖소 미경산우의 발정동기화 처리 시 번식호르몬 변화

  • 최창용 (농촌진흥청 국립축산과학원) ;
  • 허태영 (농촌진흥청 국립축산과학원) ;
  • 정영훈 (농촌진흥청 국립축산과학원) ;
  • 조용일 (농촌진흥청 국립축산과학원) ;
  • 백광수 (농촌진흥청 국립축산과학원) ;
  • 정연섭 (농촌진흥청 국립축산과학원) ;
  • 한태석 (농촌진흥청 국립축산과학원) ;
  • 이경석 (농촌진흥청 국립축산과학원) ;
  • 권응기 (농촌진흥청 국립축산과학원) ;
  • 강다원 (경상대학교 의학전문대학원 생리학교실.건강과학연구원) ;
  • 손준규 (농촌진흥청 국립축산과학원)
  • Received : 2013.03.07
  • Accepted : 2013.03.14
  • Published : 2013.03.31
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Abstract

The objective of this work was to analyze the concentrations of progesterone (P4) and estrogen (E2) hormones changed during estrus synchronization in dairy heifers. Estrus synchronization was carried out with CIDR$^{(R)}$ (Controlled Intravaginal Drug Release) devices. Corpus luteum (CL) was classified into three grades based on its size and palpable characteristics. The concentrations of P4 and E2 were measured by enzyme-amplified chemiluminescence. Serum P4 concentration was markedly low at the estrus stage (36 hrs after removal of CIDR) compared to other stages, while E2 concentration was kept high during estrus stage. The serum P4 concentration was highest in the CL classified into grade I. These results indicate that P4 concentration could be used as a criteria for determining recipients for artificial insemination or embryo transfer in dairy cattle.

Keywords

References

  1. Baek KS, Park SJ, Park SB, Kim HS, Lee HJ, Lee WS, Jeon BS, Ahn BS, Kim JG, Jeong GY, Son JK (2005): Effect of PGF2$\alpha$+PGF2$\alpha$+CIDR program on estrous response in Holstein with varying BCS in early postpartum. Korean J Emb Trans 20:279-287.
  2. Baek KS, Seong HH, Oh SJ, Yang BS, Lee MS, Jung JK (1995): Prgenancy diagnosis of Hanwoo(Korean native cattle) by serum progesterone concentration during early gestation. Korean J Animal Reprod 19:197-203.
  3. Baruselli PS, Ferreira RM, Filho MF, Nasser LF, Rodrigues CA, Bo GA (2010): Bovine embryo transfer recipient synchronisation and management in tropical environments. Reprod Fertil Dev 22:67-74.
  4. Bulman DC, McKibbin PE, Appleyard WT, Lamming GE (1978): Effect of a progesterone-releasing intravaginal device on the milk progesterone levels, vaginal flora, milk yield and fertility of cyclic and non-cyclic dairy cows. J Reprod Fertil 53:289-296. https://doi.org/10.1530/jrf.0.0530289
  5. Butler SA, Phillips NJ, Boe-Hansen GB, Bo GA, Burns BM, Dawson K, McGowan MR (2011): Ovarian responses in Bos indicus heifers treated to oxynchronize ovulation with intravaginal progesterone releasing devices, oestridiol benzoate, prostaglandin F2$\alpha$ and equine chorionic gonadotrophin. Anim Reprod Sci 129: 118-126. https://doi.org/10.1016/j.anireprosci.2011.11.001
  6. Butler SA, Phillips NJ, Boe-Hansen GB, Bo GA, Burns BM, Dawson K, McGowan MR (2012): Animallevel factors affecting ovarina function in Bos indicus heifers treated to synchronize ovulation with intravaginal progesterone-releasing devices and oesradiol benzoate. Reprod Dom Anim 47:463-471. https://doi.org/10.1111/j.1439-0531.2011.01905.x
  7. Butler WR (1998): Review: effect of protein nutrition on ovarian and uterine physiology in dairy cattle. J Dairy Sci 81:2533-2539. https://doi.org/10.3168/jds.S0022-0302(98)70146-8
  8. Chebel RC, AI-Hassan MJ, Fricke PM, Santos JEP, Lima JR, Martel CA, Stevenson JS, Garcia R, Ax RL (2010): Supplementation of progesterone via controlled internal drug release inserts during ovulation synchronization protocols in lactating dairy cows. J Dairy Sci 93:922-931. https://doi.org/10.3168/jds.2009-2301
  9. Choe C, Son JK, Cho SR, Kang D, Yeon SH, Choi SH, Kim NT, Jung YS, Kim SJ, Jung JW, Bok NH, Choi JS, Son DS (2011): Effects of corpus luteum grade of estrus synchronized recipients on pregnancy rate following embryo transfer in Korean cattle. J Emb Trans 26:33-36.
  10. Colazo MG, Dourey A, Rajamahendran R, Ambrose DJ (2013): Progesterone supplementation before timed AI increased ovulation synchrony and pregnancy per AI, and supplementation after timed AI reduced pregnancy losses in lactating dairy cows. Theriogenology 833-841.
  11. Clazo MG, Ree TO, Emmanuel DGV, Ambrose DJ (2009): Plasma luteinizing hormone concentrations in cows given repeated treatments or three different doses of gonadotropin releasing hormone. Theriogenology 71:984-992. https://doi.org/10.1016/j.theriogenology.2008.11.002
  12. Darwash AO, Lamming GE, Wooliams JA (1999): The potential for identifying heritable endocrine parameters associated with fertility in postpartum dairy cows. Anim Sci 68:333-347. https://doi.org/10.1017/S1357729800050335
  13. Folman Y, McPhee SR, Cumming IA, Davis IF, Chamley WA (1983): Conception rates in cows after various synchronisation techniques using progesterone releasing intravaginal devices. Aust Vet J 60: 44-47. https://doi.org/10.1111/j.1751-0813.1983.tb05860.x
  14. Han RX, Kim HY, Diao YF, Kim YH, Woo JS, Jin DI (2010): Progesterone and estrogen levels in Hols tein blood and milk following artificial insemination and embryo transfer. J Agri Sci 37:393-398.
  15. Hanlon DW, Davidson PJ, Hittmann AR, Joe AK (2005a): Supplementing previously treated anestrous dairy cows with progesterone does not increase first- service conception rate. Theriogenology 63:239-245. https://doi.org/10.1016/j.theriogenology.2004.04.008
  16. Hanlon DW, Jarratt GM, Davidson PJ, Millar AJ, Douglas VL (2005b): The effect of hCG administration five days after insemination on the first service conception rate of anestrous dairy cows. Theriogenology 63:1938-1945. https://doi.org/10.1016/j.theriogenology.2004.09.002
  17. Herlihy MM, Crowe MA, Diskin MG, Butler ST (2012): Effects of synchronization treatments on ovarian follicular dynamycs, corpus luteum growth, and circulating steroid hormone concentrations in lactating dairy cows. J Dairy Sci 95:743-754. https://doi.org/10.3168/jds.2011-4779
  18. Hwang WS (1984): Effect of treatment with progesterone releasing intravaginal device (PRID) on conception rate and calving interval of postpartum dairy cows. J Korean Soc Vet Clin Med 1:25-31.
  19. Jung YC, Lee GS, Kim CK, Park CS, Suh GW (1996): Dictionary of Reproduction pp 484-487.
  20. Kim CH, Bhak JS, Shin JB, Kang CH (2008): A study on the early pregnancy diagnosis by changing of plasma progesterone concentration and morphology of ovary in pregnancy and non-pregnancy cows. Korean J Vet Serv 31:397-414.
  21. Kim IH, Kim YH, Suh GH, Kang HG (2006): Factors affecting estrous exhibition and conception following a single administration of PGF2$\alpha$ in dairy cows. J Vet Clin 23:453-457.
  22. Kim SK (1985): Studies on the changes of sex hormone concentration in serum during the estrus cycle, and before and after estrus of Korean native cows. Korean J Dairy Sci 7:12-20.
  23. Kim YH, Koo JC, Oh CW, Kang SY, Yang BS, Oh SJ, Kim CN, Song JY, Kim IH (2006): In vivo embryo production and embryo transfer in Hanwoo and Jeju cattle using CIDR. Korean J Emb Trans 21:191-198.
  24. Kim YJ, Park H, Lee HL, Shin DS, Jo SW, Kim YS, Kim SH (2008): Results of embryo transfer with Hanwoo embryos produced in-vivo or in-vitro to Holstein cows as recipients. J Emb Trans 23:167-175.
  25. Kishimoto Y, Kato H, Mitani M (1987): Enzyme immunoassay of progesterone in bovine plasma and skim milk and its application to early pregnancy diagnosis. J Jpn Vet Me Assoc 40:161-164. https://doi.org/10.12935/jvma1951.40.161
  26. Lopez H, Bunch TD, Shipka MP (2002): Estrogen concentrations in milk at estrus and ovulation in dairy cows. Anim Reprod Sci 72:37-46. https://doi.org/10.1016/S0378-4320(02)00074-X
  27. Meisterling EM, Dailey RA (1987): Use of concentrations of progesterone and estradiol-17β in milk in monitoring postpartum ovarian function in dairy cows. J Dairy Sci 70:2154-2161. https://doi.org/10.3168/jds.S0022-0302(87)80268-0
  28. Nakada K, Moryyoshi M, Nakao T, Watanabe G, Taya K (2000): Changes in concentrations of plasma imminoreactive follicle-stimulating hormone, luteinizing hormone, estradiol-17$\beta$, testosterone, progesterone, and inhibin in heifer from birth to puberty. Dom Anim Endocrinol 18:57-69. https://doi.org/10.1016/S0739-7240(99)00063-6
  29. Nakao T (1980): Practical procedure for enzyme immunoassay of progesterone in bovine serum. Acta Endocrinol 93:223-227.
  30. Nakao T, Sugihashi A, Kawata K (1983): Milk progesterone levels in cows with normal or prolonged estrus cycles, referenced to an early pregnancy diagnosis. Jpn J Vet Sci 45:495-499. https://doi.org/10.1292/jvms1939.45.495
  31. Park CH, Lim WH, Suh GH, Oh KS, Son CH (2010): Relationship between the conception rate after estrus induction using PGF2$\alpha$ and other parameters in Holstein dairy cows. J Emb Trans 25:133-139.
  32. Pfeifer LF, Mapletoft RJ, Kastelic JP, Small JA, Adams GP, Dionello NJ, Singh J (2009): Effects of low versus physiologic plasma progesterone concentrations on ovarian follicular development and fertility in beef cattle. Theriogenology 72:1237-1250. https://doi.org/10.1016/j.theriogenology.2009.07.019
  33. Rantala MH, Katila T, Taponen J (2009): Effect of time interval between prostaglandin F2$\alpha$ and GnRH treatments on occurrence of short estrous cyclesin cyclic dairy heifers and cows. Theriogenology 71:930-938. https://doi.org/10.1016/j.theriogenology.2008.10.014
  34. Rounsaville TR, Oltenacu PA, Milligan RA, Foote RH (1979): Effects of heat detection, conception rate and culling policy on reproductive performance in dairy herds. J Dairy Sci 62:1435-1442. https://doi.org/10.3168/jds.S0022-0302(79)83442-6
  35. Wetteman RP, Hafs HD (1972): Estradiol and progesterone in blood serum during the bovine estrous cycle. J Ani Sci 34:1020-1024. https://doi.org/10.2527/jas1972.3461020x