Korean Journal of Ichthyology (한국어류학회지)

The Ichthyological Society of Korea (한국어류학회)
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Survival and Histological Changes in Gill of the Rockfish, Sebastes schlegeli Following Exposure to Naphthalene

나프탈렌에 노출된 조피볼락의 생존 및 아가미의 조직학적 변화

  • 조재권 (국립수산과학원 남서해수산연구소) ;
  • 김태익 (국립수산과학원 남서해수산연구소) ;
  • 손맹현 (국립수산과학원 남서해수산연구소) ;
  • 김경민 (국립수산과학원 남서해수산연구소) ;
  • 진영국 (국립수산과학원 남서해수산연구소)
  • Received : 2013.02.20
  • Accepted : 2013.03.20
  • Published : 2013.03.31
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Abstract

Rockfish, Sebastes schlegeli (total length; $10.36{\pm}0.49cm$, total weight; $16.28{\pm}1.86g$, N; 290) were exposed to various concentrations of naphthalene for 28 days. Exposure concentrations of naphthalene established control, ethanol (solvent) control, 0.5, 1.0, 1.5 and 2.0 mg Nap $L^{-1}$. After exposure, We observed survival rate, and degree of tissue change (DTC) in gill under optical microscopy. Survival rate of the rockfish was more than 90% in control, ethanol control, 0.5 and 1.0 mg Nap $L^{-1}$, whereas it decreased in 1.5 and 2.0 mg Nap $L^{-1}$ (respectively 80%, 62.2%). In histological observation of gill, hyperplasia of epithelial cells observed in all exposure groups. But no showed increase of DTC which was related to concentration. Whereas, DTC at fusion of gill lamellar, lamellar telangiectasia, stasis, aneurysm and necrosis showed dose dependent increase. Especially, fusion of gill lamellar, lamellar telangiectasia and stasis observed at more 1.0 mg Nap $L^{-1}$, and aneurysm and necrosis at more 1.5 mg Nap $L^{-1}$. These results showed naphthalene caused survival and severe change to the gill of the rockfish which was related to exposure concentration.

나프탈렌이 조피볼락의 생존과 아가미의 조직학적 변화에 미치는 영향을 관찰하였다. 실험에 사용된 조피볼락은 전장 $10.36{\pm}0.49cm$, 전중 $16.28{\pm}1.86g$, 270개체이며, 실험기간은 28일이었다. 실험농도는 6개 농도구를 설정하였다(대조구, 에탄올(solvent) 대조구, 0.5, 1.0, 1.5, 2.0 mg Nap $L^{-1}$). 실험기간 동안 사망개체를 매일 파악하여 생존율로 환산하였으며, 실험 종료 후 생존개체의 아가미는 조직표본 제작 후 조직손상의 정도(degree of tissue change (DTC))를 파악하였다. 생존율은 대조구, 에탄올 대조구, 0.5 및 1.0 mg Nap $L^{-1}$에 노출된 개체들은 90% 이상을 나타냈다. 1.5 및 2.0 mg Nap $L^{-1}$에 노출된 개체들은 노출 후 약 20일부터 급격히 감소하여 각각 80%와 62.2%를 나타냈다. 아가미의 조직학적 변화는 새엽 및 새판 상피세포의 증식이 대조구를 포함해 전 노출 농도구에서 관찰되었으며, 농도 증가에 따른 DTC의 증가는 나타나지 않았다. 하지만, 새판 상피층의 탈락 및 융합, 새판 모세혈관 확장, 울혈, 동맥류 및 괴사는 농도의존적 증가를 보였다. 특히 새판의 융합, 모세혈관 확장 및 울혈은 1.0 mg Nap $L^{-1}$ 이상의 농도에서 관찰되었으며, 동맥류와 괴사는 1.5 mg Nap $L^{-1}$ 이상의 농도에서만 관찰되었다. 이와 같은 결과는 생존율의 감소와 아가미의 조직학적 변화가 밀접한 관련이 있으며, 나프탈렌의 독성영향을 결정하는데 있어 DTC의 유용성을 보여준다.

Keywords

Acknowledgement

Grant : 능성어 대량종묘생산 기술개발

Supported by : 국립수산과학원

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