한국산림과학회지 (Journal of Korean Society of Forest Science)

  • 제102권2호
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  • Pages.264-271
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  • 2013
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  • 2586-6613(pISSN)
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  • 2586-6621(eISSN)
한국산림과학회 (Korean Society of Forest Science)
권호 표지

동위효소 표지와 cpSSR 표지를 이용한 설악산 잣나무 집단의 교배양식

Mating System in Natural Population of Pinus koraiensis at Mt. Seorak Based on Allozyme and cpSSR Markers

  • 홍용표 (국립산림과학원 산림유전자원과) ;
  • 안지영 (국립산림과학원 산림유전자원과) ;
  • 김영미 (국립산림과학원 산림유전자원과) ;
  • 홍경낙 (국립산림과학원 산림유전자원과) ;
  • 양병훈 (산림청 산림환경보호과)
  • Hong, Yong-Pyo (Division of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Ahn, Ji-Young (Division of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Kim, Young-Mi (Division of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Hong, Kyung Nak (Division of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Yang, Byeong-Hoon (Forest Environment Conservation Division, Korea Forest Service)
  • 발행 : 2013.06.30
⟨ 이전 논문 다음 논문 ⟩

초록

설악산 권금성 일대에 분포하는 잣나무 자연집단을 대상으로 동위효소와 cpSSR 표지를 이용하여 교배양식을 추정하였다. 동위효소를 이용하여 교배양식 모수를 추정한 결과 다수유전자좌 타가교배율($t_m$)은 0.882, 단일유전자좌 타가교배율($t_s$)은 0.881, 부계상관($r_p$)은 0.368로 유효 화분친 수는 평균 2.7개였다. cpSSR 표지를 이용하여 교배양식 모수를 추정한 결과, 타가교배율은 평균 0.831이었으며 유효 화분친 수는 평균 12.4개였다. 두 표지 간 평균 타가 교배율은 0.857로 침엽수종들의 타가교배율과 비슷한 수준이었다. 화분친 수는 동위효소를 이용하여 추정된 결과에 비해 cpSSR 표지로 추정된 결과가 높게 나타나서 DNA 표지의 개체식별력이 동위효소 표지에 비해 교배양식 구명에서 상대적으로 화분친을 정확하게 추정하는데 유리한 것으로 판단되었다.

Mating system parameters were estimated in a natural population of Pinus koraiensis which was located at Gwongeumseong in Mt. Seorak, South Korea. The estimated parameters from allozyme were as follows: 0.882 of multilocus outcrossing rates($t_m$), 0.881 of singlelocus outcrossing rates($t_s$), 0.368 of correlated paternity($r_p$), and 2.7 of number of effective pollen contributors. The estimated parameters from cpSSR markers were as follows: 0.831 of average of outcrossing rates and 12.4 of the average number of effective pollen contributors. The average outcrossing rate from two genetic markers was 0.857, which was similar to those estimated in other conifer species. More number of potential pollen contributors was estimated from cpSSR marker analysis compared with that estimated from allozyme marker analysis. This result sugges$t_s$ that cpSSR markers may be more useful than allozyme markers for identifying potential pollen contributors in the analysis of mating system.

키워드

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