The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
권호 표지

Mouse Single Oral Dose Toxicity Test of Scutellariae Radix Aqueous Extracts

황금의 마우스 단회 경구투여 독성시험

  • Lee, Jin-Won (Dept. of Internal Medicine, College of Oriental Medicine, Dae-Gu Haany University) ;
  • Jung, Yu-Sun (Dept. of Internal Medicine, College of Oriental Medicine, Dae-Gu Haany University) ;
  • Jung, Tae-Young (Jeahan Oriental Medical Academy, Dae-Gu Haany University) ;
  • Kim, Jong-Dae (Dept. of Internal Medicine, College of Oriental Medicine, Dae-Gu Haany University) ;
  • Choi, Hae-Yun (Dept. of Internal Medicine, College of Oriental Medicine, Dae-Gu Haany University)
  • 이진원 (대구한의대학교 내과학 교실) ;
  • 정유선 (대구한의대학교 내과학 교실) ;
  • 정태영 (대구한의대학교 제한동의학술원) ;
  • 김종대 (대구한의대학교 내과학 교실) ;
  • 최해윤 (대구한의대학교 내과학 교실)
  • Published : 2013.03.30
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Abstract

Objectives : The object of this study was to obtain acute information (single oral dose toxicity) of Scutellariae Radix Aqueous Extracts (SR; yield = 27.20%) which traditionally have been used in Korean medicine for treating various diseases including inflammatory diseases. Methods : In order to observe the 50% lethal dose ($LD_{50}$), approximate lethal dosage (ALD) and target organs, SR Aqueous Extracts were once orally administered to female and male ICR mice at dose levels of 2,000, 1,000, 500 and 0 (control) mg/kg (body weight.) according to the recommendation of KFDA Guidelines. The mortality and changes on body weight, clinical signs and gross observation were monitored during 14 days after single oral treatment of SR according to KFDA Guidelines with organ weights and histopathological observations of 14 types of principle organs. Results : After single oral treatment of SR, we could not find any mortality and toxicological evidences up to 2,000 mg/kg treated group, the limited dosages in rodents, on the body and organ weights, clinical signs, gross and histopathological observations, except for some accidental findings. Conclusions : The results obtained in this study suggest that the $LD_{50}$ and ALD of SR in both female and male mice after single oral treatment be considered as over 2,000 mg/kg because no mortalities were detected up to 2,000 mg/kg that was the highest dose recommended by KFDA and OECD, and can be safely used in clinics.

Keywords

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