YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Effects of Ginsenosides Rd and Rg1 on Proliferation of B Cells and Antibody Induction

Rd와 Rg1 인삼배당체의 B 임파구 증식 및 항체 유도 효과

  • Joo, Inkyung (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University) ;
  • Kim, Hayan (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University) ;
  • Kim, Jeonghyeon (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University) ;
  • Shehzad, Omer (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Kim, Yeong Shik (Natural Products Research Institute, College of Pharmacy, Seoul National University) ;
  • Han, Yongmoon (Department of ImmunoMicrobiology, College of Pharmacy, Dongduk Women's University)
  • 주인경 (동덕여자대학교 약학대학 면역.미생물학교실) ;
  • 김하얀 (동덕여자대학교 약학대학 면역.미생물학교실) ;
  • 김정현 (동덕여자대학교 약학대학 면역.미생물학교실) ;
  • 쉐자드 오마 (서울대학교 약학대학 천연물연구소) ;
  • 김영식 (서울대학교 약학대학 천연물연구소) ;
  • 한용문 (동덕여자대학교 약학대학 면역.미생물학교실)
  • Received : 2012.12.26
  • Accepted : 2013.02.05
  • Published : 2013.02.28
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Abstract

Induction of effective and increased levels of antibody production may be major points in vaccine development. This is especially the case when the antigenic sources are carbohydrates. Thus, in our Lab various types of formulations such as liposomal and conjugate vaccines have been researched. However, the fastidious formulation process and high costs are a problem. For this reason, there is currently a focus on utilizing immunoadjuvants. In this present study, we tested whether ginsenosides Re (a panaxdiol) and Rg1 (a panaxtriol) from Panax ginseng have immunoadjuvant activity against the cell wall of Candida albicans (CACW). The resulting data showed that Rd and Rg1 caused LPS-treated B lymphocytes to be proliferative. Rd had greater proliferation activity than that of Rg1. In the murine model of antibody production, CACW combined with Rd [CACW/Rd/IFA] or Rg1 [CACW/Rg1/IFA] increased the production of antibodies specific to C. albicans when compared to the antibody production by [CACW/IFA]-induction, which was used as a negative control (P<0.05). In the case of [CFA/Rd/IFA], the antibody production was almost twice as that of the CFA. In addition, formulations containing either had a prolonged antibody inducing activity as compared to the CFA formula. In conclusion, Rd and Rg1 have an immunologic activity, and yet Rd can be a better candidate than Rg1 for a new immunoadjuvant.

Keywords

References

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