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The Korean Society of Phycology (한국조류학회(藻類))
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Cold-tolerant strain of Haematococcus pluvialis (Haematococcaceae, Chlorophyta) from Blomstrandhalvøya (Svalbard)

  • Klochkova, Tatyana A. (Kamchatka State Technical University (KamchatGTU)) ;
  • Kwak, Min Seok (Department of Biology, Kongju National University) ;
  • Han, Jong Won (Department of Biology, Kongju National University) ;
  • Motomura, Taizo (Muroran Marine Station, Field Science Centre for Northern Biosphere, Hokkaido University) ;
  • Nagasato, Chikako (Muroran Marine Station, Field Science Centre for Northern Biosphere, Hokkaido University) ;
  • Kim, Gwang Hoon (Department of Biology, Kongju National University)
  • Received : 2013.04.15
  • Accepted : 2013.05.18
  • Published : 2013.06.15
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Abstract

A new cold-adapted Arctic strain of Haematococcus pluvialis from Blomstrandhalv${\o}$ya Island (Svalbard) is described. This strain is predominantly always in non-motile palmelloid stage. Transmission electron microscopy showed the presence of very thick cell wall and abundant lipid vesicles in the palmelloids, including red and green cells. The external morphology of the non-motile palmelloid and motile bi-flagellated cells of our strain is similar to H. pluvialis; however it differs from H. pluvialis in physiology. Our strain is adapted to live and produce astaxanthin in the low temperature ($4-10^{\circ}C$), whilst the usual growth temperature for H. pluvialis is between $20-27^{\circ}C$. Phylogenetic analysis based on 18S rRNA gene data showed that our strain nested within the Haematococcus group, forming a sister relationship to H. lacustris and H. pluvialis, which are considered synonymous. Therefore, we identified our Arctic strain as H. pluvialis.

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References

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