Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Cheonggukjang Diet and Aerobic Exercise on Lipid Metabolism and Antioxidant Enzyme in Rats

청국장 식이와 유산소 운동이 흰쥐의 지질대사 및 항산화효소에 미치는 영향

  • Kim, Sang-Woo (Department of Physical Education, Pusan National University) ;
  • Jeong, Seon-Tea (Department of Physical Education, Gyeongsang National University) ;
  • Baek, Yeong-Ho (Department of Physical Education, Pusan National University)
  • Received : 2013.02.25
  • Accepted : 2013.05.13
  • Published : 2013.05.30
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Abstract

The purpose of this study was to examine the effects of a cheonggukjang diet and aerobic exercise on lipid metabolism and antioxidant enzyme activity in rats. Thirty-two Sprague Dawley rats were randomized into a cheonggukjang diet with aerobic exercise (A), aerobic exercise (B), cheonggukjang diet (C), and control group (D). The cheonggukjang diet group consumed 20 g of 20% cheonggukjang a day with their normal diet. Exercise training consisted of treadmill running (25~40 min, 5 day/wk) and the exercise intensity was gradually increased. The results are as follows: T-C was significantly lower (p<0.05) in A compared to B and C. TG was significantly lower (p<0.001) in A compared to D. B and C were significantly lower than D. HDL-C was significantly higher (p<0.05) in C compared to D. LDL-C was not statistically different across the groups. Additionally, TBARS were not statistically different in the control or experimental groups. SOD was significantly lower (p<0.05) in A compared to D. C was significantly lower (p<0.05) that of D. CAT and GPx failed to reach the statistical difference between experimental and control groups. The major findings of this study were that aerobic exercise with a chenggukjang diet intervention improved lipid profiles and antioxidant capacity in this animal model. Therefore, a cheonggukjang diet and aerobic exercise will help to improve antioxidant capacity and prevent lifestyle related diseases.

생후 4주령 Sprague Dawley계 수컷 흰쥐를 분양 받아 청국장식이유산소운동군 8마리, 청국장식이군 8마리, 유산소운동군 8마리, 대조군 8마리, 총 32마리를 대상으로 구분하였다. 운동은 6주간, 주 5회, 경사도 0%, 속도 15~16 m/min으로 하고, 1주차 25분, 2~3주차 30분, 4~5주차 35분, 6주차 40분이 되도록 실시하였고, 청국장은 20% 청국장 첨가식이를 1일 20 g 기준으로 섭취시킨 결과는 다음과 같다. 청국장 식이와 유산소 운동은 혈중지질중 T-C, TG, HDL-C에서 긍정적인 효과가 나타났고, 항산화효소 중 SOD에서 긍정적인 효과가 나타났다. 이는 유산소 운동의 혈중지질의 개선, 항산화 효과와 청국장에 함유된 레시틴(lecithin), 사포닌(saponin), 글라이시틴(glycitin), 파이틱산(phytic acid) 등의 성분들의 시너지 작용으로 긍정적인 영향을 미친 것으로 사료된다. 그러나 LDL-C, TBARS, CAT, GPx의 차이는 나타나지 않았다. 차후 연구에서 운동강도, 빈도, 지속시간과 청국장의 섭취량 등에 변화를 준 다각적 연구가 필요할 것으로 사료된다.

Keywords

References

  1. Bae, C. W. 2003. The effect of regular aerobic running exercise on blood lipid level and serum enzyme. J Korean Soc Study Physi Edu 8, 253-266.
  2. Baek, Y. H. and Lee, S. H. 2010. The effect of aerobic exercise and allium tuberosum intake on blood lipids, MDA and antioxidant enzyme in rats. J Life Sci 20, 245-252. https://doi.org/10.5352/JLS.2010.20.2.245
  3. Choi, M. A. 2009. Effects of cheonggukjang added phellinus linteus myceria on lipid metalbolism in adult female rats. J Life Sci 19, 1679-1683. https://doi.org/10.5352/JLS.2009.19.11.1679
  4. Choi, M. L. 2000. The effects of long-term treadmill exercise on macronutrient self-selection (MNSS) and malondialdehyde (MDA). Korean Asso School Physi Edu 10, 69-72.
  5. Ferrari, R. 1994. Oxygen-freeradicals at myocardial level: effect of ischaemia and reperfusion. Adv Exp Med Biol 366, 99-111. https://doi.org/10.1007/978-1-4615-1833-4_8
  6. Food and Drug Administration. 1999. Food labeling: health claims, soy protein and coronary artery disease. Fed Regist 64, 699-733.
  7. Frei, B. 1994. Nonenzymatic antioxidant defense systems. Clarendon Press Oxford.
  8. Halliwell, B. 1991. Reactive oxygen species in living system. Sourcs biochemistry and role in human disease. Am J Med 91, 14-22.
  9. Iwai, K., Nakaya, N., Kawasaki, Y. and Matsue, H. 2002. Antioxidatine functions of natto, a kind of fermented soybeans: effect on LDL oxidation and lipid metabolism in cholesterol fed rats. J Agric Food Chem 50, 3597. https://doi.org/10.1021/jf0117199
  10. Jang, J. Y. 2010. The effect of coffee intake on fuel utilization and antioxidative activity under exercise induced oxidative stress in regularly exercised rats. Unpublished doctoral dissertation, Duksung Women's University.
  11. Jeong, S. K. 2006. The effect of 12weeks walking exercise on the plasma lipoprotein cholesterol and Apo A-I protein. Korean J Sports Sci 15, 665-667.
  12. Ji, L. L. 1993. Antioxidant enzyme response to exercise and aging. Med Sci sports Exer 25, 225-231.
  13. Jun, J. K., Jeon, B. W., Lee, S. K. Park, H. G. Jang, H. Y. and Lee, W. L. 2006. Effects of long-term endurance exercise training and L-arginine supplementation on superoxide production and antioxidants enzyme protein expression of aorta in spontaneously hypertensive rat. Korean J Exer Nutr 10, 331-340.
  14. Kelsey, F. W. and Richard, J. B. 2009. Acute exercise and oxidative stress: a 30 year history. Biomed Central. 8, 1.
  15. Kim, B. R., Koo, K. S. and Lim, I. S. 2006. The effects of aerobic exercise and chungkookjang supplementation on the blood pressure, blood lipid profiles and free radicals of hypertensive patients. Exer Sci 15, 237-245.
  16. Kim, C. G. 2002. Change of oxidative damage's factors, antioxidant enzymes & related variables for the period of vitamin mixture supplement at specific exercise intensities. Unpublished doctoral dissertation, Dankook University.
  17. Kim, D. Y. 2002. Effect of high calcium diet and aerobic exercise on blood lipid in high fat diet rats. Unpublished doctoral dissertation, Sungkyunkwan University.
  18. Kim, H. J. and Kim, Y. C. 2006. Antidiabetic and antioxidant Effects of chunggugjang powder in streptozotocin-induced diabetic rats. Korean Soc Envir Health Toxicol 21, 139-146.
  19. Kim, J. I., Kang, M. J. and Kwon, T. W. 2003. Antidibetic effect of soybean and chungkukjang. Korean Soybean Digest 20, 44-52.
  20. Kim, S. H. and Baek, Y. H. 2011. Effects of aerobic exercise and black garlic intake on blood lipids, lipid peroxidation and BAP in rats. J Life Sci 21, 923-1066. https://doi.org/10.5352/JLS.2011.21.7.1025
  21. Kim, S. H., Jung, S. H., Kim, I. H., Lee, Y. S., Lee, J. M., Kim, J. G., Lee, M. C., Choi, M. J. and Kim, D. J. 2008. The effects of chungkookjang biopolymer on blood glucose and serum lipid lowering in streptozotocin-induced diabetic rats. J Korean Soc Food Sci Nutr 37, 35-41. https://doi.org/10.3746/jkfn.2008.37.1.35
  22. Kim, Y. G., Kim, J. Y. and Lee, C. S. 2004. The effect of anti-oxidant mixed supplement on maximal exercise capacity and anti-oxidant related gene expressions. Korean J Physi Edu 43, 397-409.
  23. Koh, J. B. 2006. Effect of cheonggukjang added phellinus on lipid metabolism in hyperlipidemic rats. J Korean Soc Food Nutr 35, 410-415. https://doi.org/10.3746/jkfn.2006.35.4.410
  24. Kwon, E. Y., Jung, K. O., Moon, S. H. and Park, K. Y. 2002. Studies on enhancing chemopreventive effect of chungkookjangs. J Korean Assoc Cancer Prev 7, 200-209.
  25. Laughlin, M. H., Simpson, T., Sexton, W. L., Brown, O. R., Smith, J. K. and Korthuis, R. J. 1990. Skeletal muscle oxidative capacity antioxidant enzymes and exercise training. J Appl Physiol 68, 2337-2343.
  26. Lee, C. S., Lee, S. H., Sung, G. D. and Baek, Y. H. The effect of 4 weeks of treadmill exercise and protein diet on immunoglobulin and antioxidant enzyme in rats. J Life Sci 20, 1483-1489. https://doi.org/10.5352/JLS.2010.20.10.1483
  27. Lee, C. U. and Koh, J. B. 2006. Effects of cheonggukjang on lipid metabolism in female rats fed cholesterol diet. J Life Sci 16, 932-937. https://doi.org/10.5352/JLS.2006.16.6.932
  28. Lee, H. M., Seo, D. Y., Lee, S. H. and Baek, Y. H. 2010. Effects of exhaustive exercise and aged garlic extract supplementation on weight, adipose tissue mass, lipid profiles and oxidative stress in high fat diet induced obese rats. J Life Sci 20, 1889-1895. https://doi.org/10.5352/JLS.2010.20.12.1889
  29. Lee, S. W., Sung, N. J., Seo, K. I., Choi, S. H. and Son, M. Y. 2000. Biological activities of chungkugjang prepared with black bean and changes in phytoestrogen content during fermentation. J Korean Soc Food Sci Nutr 32, 936-941.
  30. Legina, B. F. 2009. Commentary oxidative stress reconsidered. Genes Nutr 4, 161-163. https://doi.org/10.1007/s12263-009-0131-8
  31. Nozic-Grayck, E., Suliman, H. and Piantadosi, C. 2005. Extra-cellular superoxide dismutase. Int J Bio Cell Biol 37, 2466-2471. https://doi.org/10.1016/j.biocel.2005.06.012
  32. Packer, L. 1991. Protective role of vitamin E in biological system. Am J Clin Nutr 53, 1050-1055.
  33. Park, J. H., Kim, J. M., Park, E. j. and Lee, K. H. 2008. Effects of chungkukjang added with onion on lipid and antioxidant metabolisms in rats fed high fat-cholesterol diet. J Korean Soc Food Sci Nutr 37, 1244-1250. https://doi.org/10.3746/jkfn.2008.37.10.1244
  34. Park, J. W. 2002. Change of antioxidants, lipid peroxidation, and cardiorespiratory function by resistance and aerobic exercise. Unpublished doctoral dissertation, Chonnam University.
  35. Park, Y. S. 2002. The effects of exercise training program on the body composition, health related fitness and blood lipid levels in the middle aged women. Korean Sport Res 14, 727-740.
  36. Pusan National University Institutional Animal Care and Use Committee. 2009. Useing animal experiments and care guide. Pusan National University Press.
  37. Ryu, S. H. 2001. Studies on antioxidative effects and antioxidative components of soybean and chongkukjang. Unpublished doctoral dissertation, Inje University.
  38. Seo, H. B. and Kim, H. K. 2008. Effect of chungkukjang power feeding and exercise training on blood lipids concentration and SOD activity, MDA content in sprague-dawley. Korean J Sports Sci 17, 1571-1580.
  39. Takenaka, A., Annaka, H., Kimura, Y., Aoki, H. and Igarashi, K. 2003. Reduction of paraquat-induced oxidative stress in rats by dietary soy peptide. Biosci Biotechnol Biochem 67, 278-283. https://doi.org/10.1271/bbb.67.278
  40. Tiidus, P. M., Pushkarenko, J. and Houston, M. E. 1996. Lack of antioxidant adaptation to short-term aerobic training in human muscle. Am J Physiol 271, 832-836.
  41. Tikkamen, M. J. and Adlercreutz, H. 2000. Dietary soy-derived isoplavone phytoe-trogens could they have a role in coronary heart disease prevention. Biochemical Pharmacol 60, 1-5. https://doi.org/10.1016/S0006-2952(99)00409-8
  42. You, H. J., Lee, D. S. and Kim, H. B. 2004. Chungkookjang fermentation of mixture of barley, wormwood, sea tangle, and soybean. Korean J Microbiol 40, 49-53.

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