Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Effect of Medium Composition and Growth Regulators on Mass Propagation of Virus-Free Plant from the Meristem Cultures of 'Jarang' Grape

'자랑' 포도 생장점 배양으로부터 무병주 식물 대량번식에 미치는 배지 구성물질과 생장 조절제의 영향

  • Lee, Jae Wung (Grape Research Institute, Chungcheongbuk-do Agricultural Research & Extension Services) ;
  • Lee, Yun Sang (Grape Research Institute, Chungcheongbuk-do Agricultural Research & Extension Services) ;
  • Hong, Eui Yon (Grape Research Institute, Chungcheongbuk-do Agricultural Research & Extension Services) ;
  • Lee, Seok Ho (Grape Research Institute, Chungcheongbuk-do Agricultural Research & Extension Services) ;
  • Kim, Hong Sik (Chungbuk National University) ;
  • Kim, Hag Hyun (Woosong Information Collage)
  • Received : 2013.01.08
  • Accepted : 2013.04.22
  • Published : 2013.04.30
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Abstract

This study was performed to clarify the effect of medium compositions and plant growth regulators on the shoot, root formation and growth of 'Jarang' grape for mass propagation of virus-free plant. The formation and growth of shoot were considerably favorable in half-concentration of MS medium. However, the formation of adventitious root per explants (avg. 2.1) was effective in higher concentration (two times) of MS medium. For sucrose concentration, 1% for the shoot formation, 3% for the adventitious root formation and 1% for the growth were observed as yield significant results. With the addition of 0.05% of activated carbon, the shoot growth was improved, and it was effective for the adventitious root formation and growth as well. A pH of 6.8 in the medium was the most suitable for mass propagation; the results showed significant enhancement in the number of nodes and the length of the shoot, 3.9 and 1.3 cm, respectively. The shoot growth was the most vigorous in BA 1.0 mg/L due to the impact of the growth regulator on the mass propagation in it. Consequently, 16.9 shoots per explant were formed in NAA 1.0 mg/L so good results were obtained.

본 연구는 바이러스 무병묘의 대량 증식을 위한 기초 자료를 얻고자 포도 '자랑' 품종의 신초와 뿌리의 형성 및 생장에 미치는 배지조성물질과 생장조절제의 영향을 구명하고자 수행되었다. 배지의 무기염 농도가 낮을수록 신초의 형성은 많아지는 경향으로, 특히 1/2MS배지에서 신초의 형성 및 생장이 가장 양호하였다. 부정근의 형성은 무기염의 농도가 높아질수록 양호하여 2MS배지에서 부정근의 수가 2.1개로 가장 많았다. Sucrose의 경우, 신초의 형성은 1%, 부정근의 형성은 3%, 생장은 1% 농도구에서 가장 좋았다. 활성탄의 0.05% 첨가에 의해 신초의 생장은 양호했으며, 특히, 부정근의 형성 및 생장에 효과적이었다. '자랑'의 대량 증식을 위한 배지의 pH는 6.8이 가장 적합하여 신초의 마디수와 길이가 각각 3.9개와 1.3 cm로 가장 좋았다. 기내 대량증식에 미치는 생장조절제의 영향으로 BA 1.0 mg/L에서 신초의 생장이 가장 왕성하였으며, NAA 1.0 mg/L에서 절편체당 16.9개의 신초가 형성되어 양호한 결과를 얻었다.

Keywords

References

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