Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Induction and Growth of Vegetative Stems through In Vitro Culture of Gastrodia elata

천마 기내배양을 통한 영양번식경 유도와 생장

  • Kim, Hyun Tae (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Kim, Seung Taek (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Lee, Wi Young (Department of Forest Genetic Resources, Korea Forest Research Institute) ;
  • Park, Eung Jun (Department of Forest Genetic Resources, Korea Forest Research Institute)
  • 김현태 (국립산림과학원 산림유전자원부) ;
  • 김승택 (국립산림과학원 산림유전자원부) ;
  • 이위영 (국립산림과학원 산림유전자원부) ;
  • 박응준 (국립산림과학원 산림유전자원부)
  • Received : 2013.01.31
  • Accepted : 2013.04.11
  • Published : 2013.04.30
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Abstract

Gastrodia elata has been cultivated as an important medicinal resources to treat various human diseases. One of the major problems associated with its field production is the degeneration of seed tubers, which is mainly caused by soil-borne pathogens. This study was conducted to produce disease-free seed tubers by the development of in vitro micropropagation method. First, tubers of G. elata were treated with $HgCl_2$ prior to culturing in vitro. Among various culture medium tested, water agar (WA) and WPM medium were the most effective on the induction and growth of vegetative stems. NAA ($0.1mg/{\ell}$) or TDZ ($1.0mg/{\ell}$) in WA medium showed better growth of vegetative stems compared to other plant hormones. Finally the induction and growth of vegetative stems were better in the dark compared to the light condition. In this study, we established an in vitro micropropagation system of G. elata, which might be an efficient way to increase the yield and quality of G. elata tubers in the field production.

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References

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