Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Study of Metabolic Profiling Changes in Colorectal Cancer Tissues Using 1D 1H HR-MAS NMR Spectroscopy

  • Kim, Siwon (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Lee, Sangmi (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University) ;
  • Maeng, Young Hee (School of Medicine, Jeju National University) ;
  • Chang, Weon Young (School of Medicine, Jeju National University) ;
  • Hyun, Jin Won (School of Medicine, Jeju National University) ;
  • Kim, Suhkmann (Department of Chemistry and Chemistry Institute for Functional Materials, Pusan National University)
  • Received : 2013.01.16
  • Accepted : 2013.02.20
  • Published : 2013.05.20
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Abstract

Metabolomics is a field that studies systematic dynamics and secretion of metabolites from cells to understand biological pathways based on metabolite changes. The metabolic profiling of intact human colorectal tissues was performed using high-resolution magic angle spinning (HR-MAS) NMR spectroscopy, which was unnecessary to extract metabolites from tissues. We used two different groups of samples, which were defined as normal and cancer, from 9 patients with colorectal cancer and investigated the samples in NMR experiments with a water suppression pulse sequence. We applied target profiling and multivariative statistical analysis to the analyzed 1D NMR spectra to identify the metabolites and discriminate between normal and cancer tissues. Cancer tissue showed higher levels of arginine, betaine, glutamate, lysine, taurine and lower levels of glutamine, hypoxanthine, isoleucine, lactate, methionine, pyruvate, tyrosine relative to normal tissue. In the OPLS-DA (orthogonal partial least square discriminant analysis), the score plot showed good separation between the normal and cancer groups. These results suggest that metabolic profiling of colorectal cancer could provide new biomarkers.

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