Food Science of Animal Resources (한국축산식품학회지)

Korean Society for Food Science of Animal Resources (한국축산식품학회)
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Proteomic Analysis and Protective Effects of Outer Membrane Proteins from Salmonella Gallinarum in Chickens

Salmonella Gallinarum 세포외막단백질의 프로테옴 분석 및 닭에서의 방어능 효과

  • Sun, Jisun (College of Veterinary Medicine & Institute of Veterinary Medicine, Kangwon National University) ;
  • Cho, Youngjae (College of Veterinary Medicine & Institute of Veterinary Medicine, Kangwon National University) ;
  • Jang, Joo-Hyun (KBNP Technology Institute, KBNP Inc.) ;
  • Kang, Zheng-Wu (KBNP Technology Institute, KBNP Inc.) ;
  • Han, Jang-Hyuk (KBNP Technology Institute, KBNP Inc.) ;
  • Hahn, Tae-Wook (College of Veterinary Medicine & Institute of Veterinary Medicine, Kangwon National University)
  • 선지선 (강원대학교 수의과대학, 강원대학교 동물의학종합연구소) ;
  • 조영재 (강원대학교 수의과대학, 강원대학교 동물의학종합연구소) ;
  • 장주현 ((주)고려비엔피 기술연구소) ;
  • 강정무 ((주)고려비엔피 기술연구소) ;
  • 한장혁 ((주)고려비엔피 기술연구소) ;
  • 한태욱 (강원대학교 수의과대학, 강원대학교 동물의학종합연구소)
  • Received : 2012.10.05
  • Accepted : 2013.03.28
  • Published : 2013.04.30
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Abstract

Salmonella Gallinarum (SG) is known as an important pathogen that causes fowl typhoid in chickens. To investigate SG outer-membrane proteins (OMPs) as a vaccine candidate, we used proteomic mapping and database analysis techniques with extracted OMPs. Also, extracted OMPs were evaluated in several aspects to their safety, immune response in their host and protective effects. Our research has established a proteomic map and database of immunogenic SG-OMPs used as inactive vaccine against salmonellosis in chickens. A total of 22 spots were detected by 2-dimensional gel electrophoresis and immunogenic protein analysis. Eight spots were identified by Matrix-Assisted Laser Desorption/Ionization-Time of Flight-Mass spectrometry (MALDI-TOF-MS) and peptide mass fingerprinting (PMF) and categorized into four different types of proteins. Among these proteins, OmpA is considered to be an immunogenic protein and involved in the hosts' immune system. To estimate the minimum safety dose in chickens, 35 brown layers were immunized with various concentrations of OMPs, respectively. Consequently, all chickens immunized with more than a $50{\mu}g$ dose were protected against challenges. Moreover, intramuscular administration of OMPs to chickens was more effective compared to subcutaneous administration. These results suggest that the adjuvanted SG-OMP vaccine not only induces both the humoral and cellular immune response in the host but also highly protects the hosts' exposed to virulent SG with $50{\mu}g$ OMPs extracted by our method.

Keywords

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