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Effects of Mungbean Flour Level in Combination with Microbial Transglutaminase on Physicochemical and Textural Properties of Low-salt Pork Model Sausages

  • Lee, Hong Chul (Department of Animal Science, Functional Food Research Center, Chonnam National University) ;
  • Chin, Koo Bok (Department of Animal Science, Functional Food Research Center, Chonnam National University)
  • Received : 2012.11.14
  • Accepted : 2013.03.26
  • Published : 2013.04.30

Abstract

This study was performed to evaluate the effects of various levels of mungbean flour (MF) (0-2.4%) on the quality characteristics of pork model sausages (PMS) in experiment 1 and also select the optimum level of MF to enhance the water retention and gelling properties of low-salt PMS (LSPMS) with or without microbial transglutaminase (MTGase) in experiment 2. In experiment 1, the addition of MF did not affect pH, chemical compositions (fat and moisture contents), color values, and functional properties (expressible moisture, EM (%) and cooking yield, CY (%)) of PMS. However, the addition of MF increased the chewiness of PMS and hardness if the mungbean flour at the level of more than 1.2% was incorporated. Since the interaction between the microbial transglutaminase (MTGase) treatment and MF level was not significant (p>0.05), data were pooled by different factors (MTGase treatment and MF level) in experiment 2. MF improved the water binding ability and textural springiness of LSPMS. On the other hand, MTGase treatment decreased the pH and cooking yield (%) of LSPMS, but increased most textural properties. In conclusion, the addition of MF could enhance the water retention and textural properties of PMS and LSPMS, regardless of MTGase, when it was added to over 1.2%. Based on these results, mungbean protein may interact with MTGase on the low-salt comminuted meat systems. Therefore, further study might be needed to understand the mechanisms of interaction between MTGase and functional components induced from MF.

Keywords

References

  1. AHA (2000) American heart association guidelines: Revision 2000. Circulation 102, 2284-2299. https://doi.org/10.1161/01.CIR.102.18.2284
  2. AHA (2006) Soy protein, isoflavones, and cardiovascular health. Circulation 113, 1034-1044. https://doi.org/10.1161/CIRCULATIONAHA.106.171052
  3. Anwar, E., Latif, S., Przyblski, R., Sultana, B., and Ashraf, M. (2007) Chemical composition and antioxidant activity of seeds of different cultivars of mungbean. J. Food Sci. 72, 503-510.
  4. AOAC (2000) Official methods of analysis of AOAC Intl. 17th edition. Method. 950.46, 991.36. Association of Analytical Chemists. Gaithersberg, MD, USA.
  5. Arihara, K. (2005) Strategies for designing novel functional meat products. Meat Sci. 74, 219-229.
  6. Bourne, M. C. (1978) Texture profile analysis. Food Technol. 32, 62-66.
  7. Chin, K. B. and Lee, H. C. (2002) Development of low-fat meat processing technology using interaction between meat proteins and hydrocolloids - II. Development of low-fat sausages using the results of model study. J. Korean Soc Food Sci Nutr. 31, 629-635. https://doi.org/10.3746/jkfn.2002.31.4.629
  8. Chin, K. B., Go, M. Y., and Xiong, Y. L. (2009a) Effect of soy protein substitution for sodium caseinate on the transglutaminase-indeced cold and thermal gelatin of myofibrillar protein. Food Res. Int. 42(10), 941-948. https://doi.org/10.1016/j.foodres.2009.05.008
  9. Chin, K. B., Go, M. Y., and Xiong, Y. L. (2009b) Konjac flour improved textural and water retention properties of transglutaminase-mediated, heat-induced porcine myofibrillar protein gel: Effect of salt level and transglutaminase incubation. Meat Sci. 81, 565-572. https://doi.org/10.1016/j.meatsci.2008.10.012
  10. Dube, M., Schafer, C., Neidhart, S., and Carle, R. (2007) Texturisation and modification of vegetable proteins for food applications using microbial transglutaminase. Eur. Food Res. Technol. 225, 287-299. https://doi.org/10.1007/s00217-006-0401-2
  11. Duranti, M. and Gius, C. (1997) Legume seeds: Protein content and nutritional value. Field Crops Res. 53, 31-45. https://doi.org/10.1016/S0378-4290(97)00021-X
  12. Fernandez-Gines, J. M., Fernandez-Lopez, J., Sayas-Barbera, E., and Perez-Alvarez, J. A. (2005) Meat products as functional foods: A review. J. Food Sci. 70, 37-43.
  13. Jauregui, C. A., Regenstein, J. N., and Baker, R. C. (1981) A simple centrifugal method for measuring expressible moisture, a water binding property of muscle foods. J. Food Sci. 46, 1271, 1273. https://doi.org/10.1111/j.1365-2621.1981.tb03038.x
  14. Jimenez-Colmenero, F., Carballo, J., and Cofrades, S. (2001) Healthier meat and meat products: Their role as functional foods. Meat Sci. 59, 5-13. https://doi.org/10.1016/S0309-1740(01)00053-5
  15. Kashiwagi, T., Yokoyama, K., Ishikawa, K., Ono, K., Ejima, D., Matsui, H., and Suzuki, E. (2002) Crystal structure of microbial transglutaminase from Streptoverticillium mobaraense. J. Biol. Chem. 277, 44252-44260. https://doi.org/10.1074/jbc.M203933200
  16. Ko, K. P., Park, S. K., Park, B., Yang, J. J., Cho, L. Y., Kang, C., Kim, C. S., Gwack, J., Shin, A., Kim, Y., Kim, J., Yang, H. K., Kang, D., Chang, S. H., Shin, H. R., and Yoo, K. Y. (2010) Isoflavones from phytoestrogens and gastric cancer risk: A nested case-control study within the Korean multicenter cancer cohort. Cancer Epidemiol. Biomarkers Prev. 19, 1292-1300. https://doi.org/10.1158/1055-9965.EPI-09-1004
  17. Kuraishi, C., Sakamoto, J., Yamazaki, K., Susa, Y., Kuhara, C., and Soeda, T. (1997) Production of restructured meat using microbial transglutaminase without salt or cooking. J. Food Sci. 62, 488-490, 515. https://doi.org/10.1111/j.1365-2621.1997.tb04412.x
  18. Kuraishi, C., Yamazaki, K., and Susa, Y. (2001) Transglutaminase: Its utilization in the food industry. Food Rev. Int. 17, 221-246. https://doi.org/10.1081/FRI-100001258
  19. Larkin, T., Price, W. E., and Astheimer, L. (2008) The key importance of soy isoflavone bioavailability to understanding health benefits. Crit. Rev. Food Sci. Nutr. 48, 538-552. https://doi.org/10.1080/10408390701542716
  20. Lee, H. C. and Chin, K. B. (2009) Physicochemical, textural, and sensory properties of low-fat/reduced-salt sausages as affected by salt levels and different type and level of milk proteins. Food Sci. Biotechnol. 18, 36-42.
  21. Lee, H. C. and Chin, K. B. (2011) Evaluation of various salt levels and different dairy proteins in combination with microbial transglutaminase on the quality characteristics of restructured pork ham. International J. Food Sci. Technol. 46(7), 1522-1528. https://doi.org/10.1111/j.1365-2621.2011.02654.x
  22. Lee, H. C., Park, S. Y., and Chin, K. B. (2008) Evaluation of functional and textural properties of low-salt comminuted meats as affected by different hydrocolloids and transglutaminase. Proceed. 54th Int. Congress Meat Sci. Technol. Cape Town, South Africa, p. 97.
  23. Martins, V. B. and Netto, F. M. (2006) Physicochemical and functional properties of soy protein isolate as a function of water activity and storage. Food Res. Int. 39, 145-153. https://doi.org/10.1016/j.foodres.2005.07.001
  24. Min, B. and Green, B. W. (2008) Use of microbial transglutaminase and nonmeat proteins to improve functional properties of low NaCl, phosphate-free patties made from channel catfish (Ictalurus punctatus) belly flap meat. J. Food Sci. 73(5), 218-226.
  25. Motoki, M. and Seguro, K. (1998) Transglutaminase and its use for food processing. Trends Food Sci. Technol. 9, 204-210. https://doi.org/10.1016/S0924-2244(98)00038-7
  26. Oh, H. S., Kim, J. H., and Lee, M.H. (2003) Isoflavone contents, antioxidative and fibrinolytic activities of red bean and mungbean. Korean J. Soc. Food Cookery Sci. 19, 263-270.
  27. Pietrasik, Z. and Janz, J. A. M. (2010) Utilization of pea flour, starch-rich and fiber-rich fractions in low fat bologna. Food Res. Int. 43, 602-608. https://doi.org/10.1016/j.foodres.2009.07.017
  28. Poehlman, J. M. (1991) The mungbean. Colorado, USA: Westview Press.
  29. Rakosky, J. (1970) Soy products for the meat industry. J. Agri. Food Chem. 18, 1005-1009. https://doi.org/10.1021/jf60172a032
  30. SACN (2003) Salt and health. Scientific advisory committee on nutrition. The Stationery Office, Norwich, UK.
  31. Sakamoto, H., Kumazawa, Y., and Motoki, M. (1994) Strength of protein gels prepared with microbial transglutaminase as related to reaction conditions. J. Food Sci. 59, 866-871. https://doi.org/10.1111/j.1365-2621.1994.tb08146.x
  32. Sanjeewa, W. G. T., Wanasundara, J. P. D., Pietrasik, Z., and Shand, P. J. (2010) Characterization of chickpea (Cicer arietinum L.) flours and application in low-fat pork bologna as a model system. Food Res. Int. 43, 617-626. https://doi.org/10.1016/j.foodres.2009.07.024
  33. Sosulski, F., Garratt, M. D., and Slinkard, A. E. (1976) Functional properties of ten legume flours. J. Can. Inst. Food Sci. Technol. 9, 66-69. https://doi.org/10.1016/S0315-5463(76)73614-9
  34. SPSS (2010) PASW Statistics 18 for windows. Chicago IL, USA.
  35. Trout, G. R. and Schmidt, G. R. (1984) Effects of reduced salt (NaCl) levels on sensory and instrumental evaluation of frankfurters. J. Food Sci. 49, 687-694. https://doi.org/10.1111/j.1365-2621.1984.tb13189.x

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