Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Stimulation of Phospholipase D in HepG2 Cells After Transfection Using Cationic Liposomes

  • Lee, Sang Yoon (Chronic Inflammatory Disease Research Center, Ajou University School of Medicine) ;
  • Lee, Yan (Department of Chemistry, College of Natural Sciences, Seoul National University) ;
  • Choi, Joon Sig (Department of Biochemistry, College of Natural Sciences, Chungnam National University) ;
  • Park, Jong Sang (Department of Chemistry, College of Natural Sciences, Seoul National University) ;
  • Choi, Myung-Un (Department of Chemistry, College of Natural Sciences, Seoul National University)
  • Received : 2013.01.09
  • Accepted : 2013.01.14
  • Published : 2013.03.20
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Abstract

Lipid events in liposome-mediated transfection (lipofection) are largely unknown. Here we studied whether phospholipase D (PLD), an important enzyme responsible for phospholipid breakdown, was affected during lipofection of HepG2 cells with a luciferase plasmid. Synthetic cholesterol (Chol) derivatives, including $3{\beta}$[L-ornithinamide-carbamoyl]Chol, [polyamidoamine-carbamoyl]Chol and $3{\beta}$[N-(N',N'-dimethylaminoethane)-carbamoyl]Chol, and a cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride were mixed with a helper lipid dioleoylphosphatidylethanolamine to form respective cationic liposomes. All cationic liposomes were found to stimulate PLD. Although orders of magnitude effects of the cationic liposomes on PLD stimulation did not consistently match those on cytotoxicity and luciferase expression, a causal relationship between PLD activation and cytotoxic effect was remarkable. PLD stimulation by the cationic liposomes was likely due to their amphiphilic characters, leading to membrane perturbation, as supported by similar results obtained with other membrane-perturbing chemicals such as oleate, melittin, and digitonin. Our results suggest that lipofection induces cellular lipid changes such as a PLD-driven phospholipid turnover.

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References

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