Biomolecules & Therapeutics

The Korean Society of Applied Pharmacology (한국응용약물학회)
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Hesperetin Stimulates Cholecystokinin Secretion in Enteroendocrine STC-1 Cells

  • Kim, Hye Young (Metabolism and Nutrition Research Group, Division of Metabolism and Functionality Research, Korea Food Research Institute) ;
  • Park, Min (Metabolism and Nutrition Research Group, Division of Metabolism and Functionality Research, Korea Food Research Institute) ;
  • Kim, Kyong (Metabolism and Nutrition Research Group, Division of Metabolism and Functionality Research, Korea Food Research Institute) ;
  • Lee, Yu Mi (Metabolism and Nutrition Research Group, Division of Metabolism and Functionality Research, Korea Food Research Institute) ;
  • Rhyu, Mee Ra (Metabolism and Nutrition Research Group, Division of Metabolism and Functionality Research, Korea Food Research Institute)
  • Received : 2012.10.08
  • Accepted : 2013.01.22
  • Published : 2013.03.31
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Abstract

Hesperetin (3',5,7-trihydroxy 4'-methoxyflavanone) and its glycoside hesperidin (hesperetin 7-rhamnoglucoside) in oranges have been reported to possess pharmacological effects related to anti-obesity. However, hesperetin and hesperidin have not been studied on suppressive effects on appetite. This study examined that hesperetin and hesperidin can stimulate the release of cholecystokinin (CCK), one of appetite-regulating hormones, from the enteroendocrine STC-1 cells, and then examined the mechanisms involved in the CCK release. Hesperetin significantly and dose-dependently stimulated CCK secretion with an $EC_{50}$ of 0.050 mM and increased the intracellular $Ca^{2+}$ concentrations ($[Ca^{2+}]_i$) compared to the untreated control. The stimulatory effect by hesperetin was mediated via the entry of extracellular $Ca^{2+}$ and the activation of TRP channels including TRPA1. These results suggest that hesperetin can be a candidate biomolecule for the suppression of appetite and eventually for the therapeutics of obesity.

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References

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