Korean Journal of Soil Science and Fertilizer (한국토양비료학회지)

Korean Society of Soil Science and Fertilizer (한국토양비료학회)
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Physiological Response and Cadmium Accumulation of MuS1 Transgenic Tobacco Exposed to High Concentration of Cd in Soil: Implication to Phytoremediation of Metal Contaminated Soil

토양 중 고농도 카드뮴에 노출된 MuS1 형질전환 담배 (Nicotiana tabacum cv. Xanthi)의 생리적 반응 및 카드뮴 축적: 식물학적 오염토양정화를 위한 형질전환 식물 탐색

  • Jeoung, Yoon-Hwa (Department of Environment Horticulture, The University of Seoul) ;
  • Kim, Young-Nam (Department of Ecology, Lincoln University) ;
  • Kim, Kwon-Rae (Department of Agronomy and Medicinal Plant Resources, Gyeongnam National University of Science and Technology) ;
  • Kim, Kye-Hoon (Department of Environment Horticulture, The University of Seoul)
  • 정윤화 (서울시립대학교 환경원예학과) ;
  • 김영남 (링컨대학교 생태학과) ;
  • 김권래 (경남과학기술대학교 농학한약자원학부) ;
  • 김계훈 (서울시립대학교 환경원예학과)
  • Received : 2013.01.16
  • Accepted : 2013.02.05
  • Published : 2013.02.28
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Abstract

The objective of this study was to understand the physiological response and cadmium accumulation of MuS1 transgenic tobacco exposed to high concentration of Cd in soil. For this, a pot experiment was carried out in a greenhouse for a month, with two lines of MuS1 transgenic tobaccos (S4 and S6) and non-transgenic tobacco cultivated in the soils spiked at three different Cd concentrations (0, 60 and 180 mg $kg^{-1}$). Both transgenic and non-transgenic tobacco showed visible toxic symptoms such as chlorosis and leaf roll as treated concentration increased. The net photosynthetic rates of MuS1 plants (S4 and S6) exposed at 180 mg $kg^{-1}$ Cd were 6.3 and $7.7{\mu}mol\;m^{-2}s^{-1}$, being higher than those of the non-transgenic plant ($4.8{\mu}mol\;m^{-2}s^{-1}$). Values of stomatal conductance of MuS1 transgenic plants (0.05 and 0.008 mmol $H_2O\;m^{-2}s^{-1}$) were also higher than those of non-transgenic plant (0.03 mmol $H_2O\;m^{-2}s^{-1}$). In addition, fresh and dry weights of MuS1 transgenic plants were heavier than those of non-transgenic plant. Likewise, MuS1 transgenic plants appeared to be better physiological performance than non-transgenic tobacco when exposed at high concentration of Cd in soil. With regard to metal accumulation, MuS1 transgenic tobaccos accumulated more Cd in their roots than non-transgenic tobacco implying that MuS1 transgenic tobacco is suggested to be used for phytostabilization of heavy metals.

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References

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