Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Bacterial Community Structure Shift Driven by Salinity: Analysis of DGGE Band Patterns from Freshwater to Seawater of Hyeongsan River, Korea

염도의 변화에 따른 미생물 군집의 변화: 경북 형산강 하류 미생물 군집 변화의 DGGE pattern 분석

  • Beck, Bo Ram (East Sea Rim Marine and Fisheries Research Institute, Handong Global University) ;
  • Holzapfel, Wilhelm (East Sea Rim Marine and Fisheries Research Institute, Handong Global University) ;
  • Hwang, Cher Won (Global Leadership School, Handong Global University) ;
  • Do, Hyung Ki (East Sea Rim Marine and Fisheries Research Institute, Handong Global University)
  • 백보람 (한동대학교 환동해 해양수산연구소) ;
  • ;
  • 황철원 (한동대학교 GLS 학부) ;
  • 도형기 (한동대학교 환동해 해양수산연구소)
  • Received : 2012.11.15
  • Accepted : 2013.03.19
  • Published : 2013.03.30
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Abstract

The influence of a gradual increase in salinity on the diversity of aquatic bacterial in rivers was demonstrated. The denaturing gradient gel electrophoresis (DGGE) was used to analyze the bacterial community shift downstream in the Hyeongsan River until it joins the open ocean. Four water samples were taken from the river showing the salinity gradients of 0.02%, 1.48%, 2.63%, and 3.62%. The samples were collected from four arbitrary stations in 2.91 km intervals on average, and a DGGE analysis was performed. Based on the results of this analysis, phylogenetic similarity identification, tree analysis, and a comparison of each station were performed. The results strongly suggested that the response of the bacterial community response was concomitant to gradual changes in salinity, which implies that salt concentration is a major factor in shifting the microbiota in aquatic habitats. The results also imply a huge diversity in a relatively small area upstream from the river mouth, compared to that in open oceans or coastal regions. Therefore, areas downstream towards a river mouth or delta are could be good starting points in the search for new bacterial species and strains ("biotypes").

강의 하류지역에서 미생물의 군집이 점진적인 염도의 증가에 따라 변한다는 것을 실험적으로 보기 위하여, 경북 형산강의 하류에서부터 연안해역으로 유입되는 곳까지 약 2.91 km 간격으로 0.02%, 1.48%, 2.63%, 3.62%의 염분을 포함하는 물 시료를 얻어 denaturing gradient gel electrophoresis (DGGE)를 수행하였다. 계통분석, 계통수 및 각 시료간의 연관성을 조사한 결과, 미생물 군집의 변화가 염분의 증가에 따라 점진적으로 변화하는 것을 확인하였으며, 이는 염분의 농도가 미생물 군집에 큰 영향을 미치는 요소임을 제시한다. 덧붙여 연안 지역이나 다른 수계환경에 비해 하류지역은 염분의 점진적인 변화로 인해 좁은 면적에 비하여 미생물 다양성이 크고, 이는 곧 특이하고 새로운 종을 찾기에 좋은 장소임을 시사하였다.

Keywords

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