Applied Microscopy

  • 제43권1호
  • /
  • Pages.9-13
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  • 2013
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  • 2287-5123(pISSN)
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  • 2287-4445(eISSN)
한국현미경학회 (Korean Society of Microscopy)
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Biological Applications of Helium Ion Microscopy

  • Kim, Ki Woo (School of Ecology and Environmental System, Kyungpook National University)
  • 투고 : 2013.02.13
  • 심사 : 2013.03.11
  • 발행 : 2013.03.30
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초록

The helium ion microscope (HIM) has recently emerged as a novel tool for imaging and analysis. Based on a bright ion source and small probe, the HIM offers advantages over the conventional field emission scanning electron microscope. The key features of the HIM include (1) high resolution (ca. 0.25 nm), (2) great surface sensitivity, (3) great contrast, (4) large depth-of-field, (5) efficient charge control, (6) reduced specimen damage, and (7) nanomachining capability. Due to the charge neutralization by flood electron beam, there is no need for conductive metal coating for the observation of insulating biological specimens by HIM. There is growing evidence that the HIM has substantial potential for high-resolution imaging of uncoated insulating biological specimens at the nanoscale.

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참고문헌

  1. Alkemade P F A, Koster E M, Van Veldhoven E, and Maas D J (2012) Imaging and nanofabrication with the helium ion microscope of the Van Leeuwenhoek Laboratory in Delft. Scanning 34, 90-100. https://doi.org/10.1002/sca.21009
  2. Bazou D, Behan G, Reid C, Boland J J, and Zhang H Z (2011) Imaging of human colon cancer cells using He-Ion scanning microscopy. J. Microsc. 242, 290-294. https://doi.org/10.1111/j.1365-2818.2010.03467.x
  3. Bell D C (2009) Contrast mechanisms and image formation in helium ion microscopy. Microsc. Microanal. 15, 147-153. https://doi.org/10.1017/S1431927609090138
  4. Boden S A, Asadollahbaik A, Rutt H N, and Bagnall D M (2012) Helium ion microscopy of Lepidoptera scales. Scanning 34, 107-120. https://doi.org/10.1002/sca.20267
  5. Bosco G L (2011) Imaging in the modern age. Trends Analyt. Chem. 30, 1189-1211. https://doi.org/10.1016/j.trac.2011.07.009
  6. Chen X, Udalagama C N B, Chen C-B, Bettiol A A , Pickard D S, Venkatesan T, and Watt F (2011) Whole-cell imaging at nanometer resolutions using fast and slow focused helium ions. Biophys. J. 101, 1788-1793. https://doi.org/10.1016/j.bpj.2011.08.028
  7. Economou N P, Notte J A, and Thompson W B (2012) The history and development of the helium ion microscope. Scanning 34, 83-89. https://doi.org/10.1002/sca.20239
  8. Kim K W (2012) Helium ion microscopy of uncoated pine leaves. Appl. Microsc. 42, 147-150. https://doi.org/10.9729/AM.2012.42.3.147
  9. Morgan J, Notte J, Hill R, and Ward B (2006) An introduction to the helium ion microscope. Microsc. Today 14(4), 24-31.
  10. Postek M T and Vladar A E (2008) Helium ion microscopy and its application to nanotechnology and nanometrology. Scanning 30, 457-462. https://doi.org/10.1002/sca.20129
  11. Scipioni L (2009) Principles and applications of helium ion microscopy. Available from: http://www.microscopy-analysis.com/features/principles-and-applications-helium-ion-microscopy/.
  12. Scipioni L, Stern L, and Notte J (2007) Applications of the helium ion microscope. Microsc. Today 15(6), 12-15.
  13. Vanden Berg-Foels W S, Scipioni L, Huynh C, and Wen X (2012) Helium ion microscopy for high-resolution visualization of the articular cartilage collagen network. J. Microsc. 246, 168-176. https://doi.org/10.1111/j.1365-2818.2012.03606.x
  14. Ward B W, Notte J A, and Economou N P (2006) Helium ion microscope: a new tool for nanoscale microscopy and metrology. J. Vac. Sci. Technol. B 24, 2871-2874. https://doi.org/10.1116/1.2357967