KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지
DOI QR코드

DOI QR Code

Study on Online Monitoring of Dissolved Oxygen, pH and Cell Concentration in E. coli Cultivation Processes Using MABOOMSTM

마이크로플레이트 기반 생물반응기 시스템 (MABOOMSTM)을 이용한 대장균 배양공정에서 용존산소, pH 및 세포농도의 온라인 모니터링 연구

  • Sohn, Ok-Jae (School of Applied Chemical Engineering, Research Institute for Catalysis, Chonnam National University) ;
  • Rhee, Jong Il (School of Applied Chemical Engineering, Research Institute for Catalysis, Chonnam National University)
  • 손옥재 (전남대학교 공과대학 응용화학공학부, 촉매연구소) ;
  • 이종일 (전남대학교 공과대학 응용화학공학부, 촉매연구소)
  • Received : 2012.12.10
  • Accepted : 2013.01.29
  • Published : 2013.02.27
Download PDF
⟨ Previous Next ⟩

Abstract

Dissolved oxygen, pH and cell concentration have been online monitored in cultivation processes with Escherichia coli by using a $MABOOMS^{TM}$ (microplate-based bioreactor with optical online monitoring systems). Fluorescent sensing membranes containing Ru ${(dpp)_3}^{2+}$ or HPTS were prepared with GA sol-gel matrix and coated into a well of a 24-well microplate. Fluorescence intensity was measured and correlated to the dissolved oxygen or pH. Cell concentrations were also online monitored by measuring optical reflectance at 650 nm. A well of a 24-well microplate could also be divided into 4 parts, each of which was coated with fluorescent sensing membranes for the detection of dissolved oxygen or pH. The 24-well microplate coated with fluorescent sensing membranes or a 4-divided sensing membrane. was used to online monitor the dissolved oxygen, pH and cell concentration during E. coli cultivations. The online monitoring results showed the characteristics of cell growth in cultivation processes very well.

Keywords

References

  1. D. T. Stitt, M. S. Nagar, T. A. Haq, and M. R. Timmins (2002) Determination of growth rate of microorganism in broth from oxygen-sensitive fluorescence plate reader measurements. Bio Tech-niques 32: 684-689.
  2. G. T. John, D. Goelling, I. Klimant, H. Schneider, and E. Heinzle (2003) pH-sensing 96-well microtitre plates for the characterization of acid production by dairy starter cultures. Journal of Dairy Research 70: 327-333. https://doi.org/10.1017/S0022029903006344
  3. G. T. John, I. Klimant, C. Wittmann, and E. Heinzle (2003) Integrated optical sensing of dissolved oxygen in microtiter plates: a novel tool for microbial cultivation. Biotechnology and Bioengineering 81: 829-836. https://doi.org/10.1002/bit.10534
  4. T. Anderlei and J. Buchs (2001) Device for sterile online measurement of the oxygen transfer rate in shaking flasks. Biochemical Engineering Journal 7: 157-162. https://doi.org/10.1016/S1369-703X(00)00116-9
  5. L. Tolosa, Y. Kostov, P. Harms, and G. Rao (2002) Noninvasive measurement of dissolved oxygen in shake flasks. Biotechnology and Bioengineering 80: 594-597. https://doi.org/10.1002/bit.10409
  6. A. Gupta and G. Rao (2003) A study of oxygen transfer in shake flasks using a non-invasive oxygen sensor. Biotechnology and Bioengineering 84: 351-358. https://doi.org/10.1002/bit.10740
  7. D. Weuster-Botz, J. Altenbach-Rehm, and M. Arnold (2001) Parallel substrate feeding and pH-control in shaking-flasks. Biochemical Engineering Journal 7: 163-170. https://doi.org/10.1016/S1369-703X(00)00117-0
  8. D. Weuster-Botz, R. Puskeiler, A. Kusterer, K. Kaufmann, G. T. John, and M. Arnold (2005) Methods and milliliter-scale device for high-throughput bioprocess design. Bioprocess and Biosystems Engineering 28: 109-119. https://doi.org/10.1007/s00449-005-0011-6
  9. J. I. Rhee (2008) Korea Patent, No. 10-0860958-0000.
  10. C.-K. Kim and J. I. Rhee (2009) On-line Monitoring of microorganism cultivation processes using optical sensing membranes for simultaneous detection of dissolved oxygen and pH. KSBB Journal 24: 106-112.
  11. S.-Y. Kim, C.-K. Kim, O.-J. Sohn, and J. I. Rhee (2009) Monitoring of pH and dissolved oxygen in microorganism fermentation processes using 24-well microplate. KSBB Journal 24: 207-211.

Cited by

  1. Study on Cell Growth Characteristics with Culture Medium Components by Using MABOOMSTM vol.28, pp.1, 2013, https://doi.org/10.7841/ksbbj.2013.28.1.31