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The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Activation of Thromboxane Receptor Mediates Interleukin-8 Expression in Endothelial Cells

트롬복산 수용체 활성화가 인터루킨-8 발현에 미치는 영향

  • Jeon, Hwa-Jin (Department of Oral Physiology, School of Dentistry, Yangsan Campus of Pusan National University) ;
  • Kim, Su-Ryun (Department of Oral Physiology, School of Dentistry, Yangsan Campus of Pusan National University) ;
  • Park, Hyun-Joo (Department of Oral Physiology, School of Dentistry, Yangsan Campus of Pusan National University) ;
  • Kim, Mi-Kyoung (Department of Oral Physiology, School of Dentistry, Yangsan Campus of Pusan National University) ;
  • Kim, Do-Won (Department of Oral Physiology, School of Dentistry, Yangsan Campus of Pusan National University) ;
  • Bae, Soo-Kyung (Department of Dental Pharmacology, School of Dentistry, Yangsan Campus of Pusan National University) ;
  • Bae, Moon-Kyoung (Department of Oral Physiology, School of Dentistry, Yangsan Campus of Pusan National University)
  • 전화진 (부산대학교 치의학전문대학원 구강생리학교실) ;
  • 김수륜 (부산대학교 치의학전문대학원 구강생리학교실) ;
  • 박현주 (부산대학교 치의학전문대학원 구강생리학교실) ;
  • 김미경 (부산대학교 치의학전문대학원 구강생리학교실) ;
  • 김도원 (부산대학교 치의학전문대학원 구강생리학교실) ;
  • 배수경 (부산대학교 치의학전문대학원 치과약리학교실) ;
  • 배문경 (부산대학교 치의학전문대학원 구강생리학교실)
  • Received : 2013.01.02
  • Accepted : 2013.01.29
  • Published : 2013.02.27
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Abstract

Thromboxane $A_2$ ($TXA_2$) is one of major proinflammatory mediators, plays an important role in the development of vascular inflammatory diseases. $TXA_2$ acting through the thromboxane receptor regulates multiple pathways and genes in a variety of cells. In this study, we report that the activation of thromboxane receptor with U46619 increases the interleukin-8 (IL-8) mRNA in vascular endothelial cells. We also demonstrated that U46619 produces the activations of extracellular signal-regulated kinase 1/2 (ERK1/2) and p38 mitogen-activated protein kinase (MAPK), which is required for endothelial IL-8 production. And U46619 enhanced mRNA stability of IL-8 transcripts in endothelial cells. Moreover, inhibition of ERK1/2 or p38MAPK reduced monocyte adhesion to aortic endothelium stimulated by U46619. Therefore, these results suggest that activation of thromboxane receptor promotes the expression of IL-8 via ERK1/2 and p38MAPK activation in endothelial cells.

Keywords

References

  1. Stevens, T., J. G. Garcia, D. M. Shasby, J. Bhattacharya, and A. B. Malik (2000) Mechanisms regulating endothelial cell barrier function. Am. J. Physiol. Lung Cell. Mol. Physiol. 3: L419-422.
  2. Ley, K., C. Laudanna, M. I. Cybulsky, and S. Nourshargh (2007) Getting to the site of inflammation: the leukocyte adhesion cascade updated. Nat. Rev. Immunol. 9: 678-689.
  3. Muller, W. A. (2003) Leukocyte-endothelial-cell interactions in leukocyte transmigration and the inflammatory response. Trends Immunol. 6: 327-334.
  4. Bates, D. O. and S. J. Harper (2002) Regulation of vascular permeability by vascular endothelial growth factors. Vascul Pharmacol. 4-5: 225-237.
  5. Carmeliet, P. (2003) Angiogenesis in health and disease. Nat. Med. 6: 653-660. https://doi.org/10.1038/nn0703-653
  6. Carmeliet, P. and R. K. Jain (2000) Angiogenesis in cancer and other diseases. Nature 6801: 249-257.
  7. Otrock, Z. K., R. A. Mahfouz, J. A. Makarem, and A. I. Shamseddine (2007) Understanding the biology of angiogenesis: review of the most important molecular mechanisms. Blood Cells Mol. Dis. 2: 212-220.
  8. Liu, W., S. A. Ahmad, N. Reinmuth, R. M. Shaheen, Y. D. Jung, F. Fan, and L. M. Ellis (2000) Endothelial cell survival and apoptosis in the tumor vasculature. Apoptosis 4: 323-328.
  9. Iniguez, M. A., C. Cacheiro-Llaguno, N. Cuesta, M. D. Diaz- Munoz, and M. Fresno (2008) Prostanoid function and cardiovascular disease. Arch. Physiol. Biochem. 3: 201-209.
  10. Alfranca, A., M. A. Iniguez, M. Fresno, and J. M. Redondo (2006) Prostanoid signal transduction and gene expression in the endothelium: role in cardiovascular diseases. Cardiovasc. Res. 3: 446-456.
  11. Ishizuka, T., M. Kawakami, T. Hidaka, Y. Matsuki, M., Takamizawa, K. Suzuki, A. Kurita, and Nakamura, H. (1998). Stimulation with thromboxane A2 (TXA2) receptor agonist enhances ICAM-1, VCAM-1 or ELAM-1 expression by human vascular endothelial cells. Clin. Exp. Immunol. 3: 464-470.
  12. Nie, D., M. Lamberti, A. Zacharek, L. Li, K. Szekeres, K. Tang, Y. Chen, and K. V. Honn (2000) Thromboxane A(2) regulation of endothelial cell migration, angiogenesis, and tumor metastasis. Biochem. Biophys. Res. Commun. 1: 245-251.
  13. Daniel, T. O., H. Liu, J. D. Morrow, B. C. Crews, and L. J. Marnett (1999) Thromboxane A2 is a mediator of cyclooxygenase- 2- dependent endothelial migration and angiogenesis. Cancer Res. 18: 4574-4577.
  14. Kim, S. R., S. K. Bae, H. J. Park, M. K. Kim, K. Kim, S. Y. Park, H. O. Jang, I. Yun, Y. J. Kim, M. A. Yoo, and M. K. Bae (2010) Thromboxane A(2) increases endothelial permeability through upregulation of interleukin-8. Biochem. Biophys. Res. Commun. 3:413-419.
  15. Ashton, A. W., Y. Cheng, A. Helisch, and J. A. Ware (2004) Thromboxane A2 receptor agonists antagonize the proangiogenic effects of fibroblast growth factor-2: role of receptor internalization, thrombospondin-1, and alpha(v)beta3. Circ. Res. 6: 735-742.
  16. Michel, F., J. S. Silvestre, L. Waeckel, S. Corda, T. Verbeuren, J. P. Vilaine, M. Clergue, M. Duriez, and B. I. Levy (2006). Thromboxane A2/prostaglandin H2 receptor activation mediates angiotensin II-induced postischemic neovascularization. Arterioscler. Thromb. Vasc. Biol. 3: 488-493.
  17. Nakahata, N. (2008) Thromboxane A2: physiology/pathophysiology, cellular signal transduction and pharmacology. Pharmacol. Ther. 1: 18-35.
  18. Dogne, J. M., J. Hanson, and D. Pratico (2005) Thromboxane, prostacyclin and isoprostanes: therapeutic targets in atherogenesis. Trends Pharmacol. Sci. 12: 639-644.
  19. Baggiolini, M., B. Dewald, and B. Moser (1994) Interleukin-8 and related chemotactic cytokines-CXC and CC chemokines. Adv. Immunol. 97-179.
  20. Hoffmann, E., O. Dittrich-Breiholz, H. Holtmann, and M. Kracht (2002). Multiple control of interleukin-8 gene expression. J. Leukoc. Biol. 5: 847-855.
  21. Li, A., S. Dubey, M. L. Varney, B. J. Dave, and R. K. Singh (2003) IL-8 directly enhanced endothelial cell survival, proliferation, and matrix metalloproteinases production and regulated angiogenesis. J. Immunol. 6: 3369-3376.
  22. Biffl, W. L., E. E. Moore, F. A. Moore, V. S. Carl, R. J. Franciose, and A. Banerjee (1995) Interleukin-8 increases endothelial permeability independent of neutrophils. J. Trauma 1: 98-102; discussion 102-3.
  23. Reutershan, J., M. A. Morris, T. L. Burcin, D. F. Smith, D. Chang, M. S. Saprito, and K. Ley (2006) Critical role of endothelial CXCR2 in LPS-induced neutrophil migration into the lung. J. Clin. Invest. 3: 695-702.
  24. Bayat, H., S. Xu, D. Pimentel, R. A. Cohen, and B. Jiang (2008) Activation of thromboxane receptor upregulates interleukin (IL)- 1beta-induced VCAM-1 expression through JNK signaling. Arterioscler. Thromb. Vasc. Biol. 1: 127-134.
  25. Miggin, S. M. and B. T. Kinsella (2001) Thromboxane A(2) receptor mediated activation of the mitogen activated protein kinase cascades in human uterine smooth muscle cells. Biochim. Biophys. Acta 1-2: 147-162.
  26. Subramaniam, D., S. Ramalingam, R. May, B. K. Dieckgraefe, D. E. Berg, C. Pothoulakis, C. W.Houchen, T. C.Wang, and S. Anant (2008) Gastrin-mediated interleukin-8 and cyclooxygenase-2 gene expression: differential transcriptional and posttranscriptional mechanisms. Gastroenterology 4: 1070-1082.
  27. Winzen, R., M. Kracht, B. Ritter, A. Wilhelm, C. Y.Chen, A. B. Shyu, M. Muller, M. Gaestel, K. Resch, and H. Holtmann (1999) The p38 MAP kinase pathway signals for cytokine-induced mRNA stabilization via MAP kinase-activated protein kinase 2 and an AU-rich region-targeted mechanism. EMBO J. 18: 4969-4980. https://doi.org/10.1093/emboj/18.18.4969
  28. Babu, P. V., H. Si, Z. Fu, W. Zhen, and D. Liu (2012) Genistein prevents hyperglycemia-induced monocyte adhesion to human aortic endothelial cells through preservation of the cAMP signaling pathway and ameliorates vascular inflammation in obese diabetic mice. J. Nutr. 4: 724-730.
  29. Srinivasan, S., M. Yeh, E. C. Danziger, M. E. Hatley, A. E. Riggan, N. Leitinger, J. A.Berliner, and C. C. Hedrick (2003) Glucose regulates monocyte adhesion through endothelial production of interleukin-8. Circ. Res. 4: 371-377.
  30. Kim, S. R., Y. H. Jung, H. J. Park, M. K. Kim, J. W. Jeong, H. O. Jang, I. Yun, S. K. Bae, and M. K. Bae (2012) Upregulation of thromboxane synthase mediates visfatin-induced interleukin-8 expression and angiogenic activity in endothelial cells. Biochem. Biophys. Res. Commun. 4: 662-668.